An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK.

Colonna-Romano S, Arnold W, Schlüter A, Boistard P, Pühler A, Priefer UB (1990)
Mol Gen Genet 223(1): 138-147.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Colonna-Romano, S.; Arnold, WalterUniBi; Schlüter, AndreasUniBi ; Boistard, P.; Pühler, AlfredUniBi ; Priefer, U. B.
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
A 1.9 kb DNA region of Rhizobium leguminosarum biovar viciae strain VF39 capable of promoting microaerobic and symbiotic induction of the Rhizobium meliloti fixN gene was identified by heterologous complementation. Sequence analysis of this DNA region revealed the presence of two complete open reading frames, orf240 and orf114. The deduced amino acid sequence of orf240 showed significant homology to Escherichia coli Fnr and R. meliloti FixK. The major difference between ORF240 and FixK is the presence of 21 N-terminal amino acids in ORF240 that have no counterpart in FixK. A similar protein domain is also present in E. coli Fnr and is essential for the oxygen-regulated activity of this protein. Analysis of the nucleotide sequence upstream of orf240 revealed a motif similar to the NtrA-dependent promoter consensus sequence, as well as two DNA regions resembling the Fnr consensus binding sequence. A Tn5-generated mutant in orf240 lost the ability to induce the R. meliloti fixN-lacZ fusion. Interestingly, this mutant was still capable of nitrogen fixation but showed reduced nitrogenase activity.
Erscheinungsjahr
1990
Zeitschriftentitel
Mol Gen Genet
Band
223
Ausgabe
1
Seite(n)
138-147
ISSN
0026-8925
eISSN
1617-4623
Page URI
https://pub.uni-bielefeld.de/record/1668756

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Colonna-Romano S, Arnold W, Schlüter A, Boistard P, Pühler A, Priefer UB. An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK. Mol Gen Genet. 1990;223(1):138-147.
Colonna-Romano, S., Arnold, W., Schlüter, A., Boistard, P., Pühler, A., & Priefer, U. B. (1990). An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK. Mol Gen Genet, 223(1), 138-147. https://doi.org/10.1007/BF00315806
Colonna-Romano, S., Arnold, Walter, Schlüter, Andreas, Boistard, P., Pühler, Alfred, and Priefer, U. B. 1990. “An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK.”. Mol Gen Genet 223 (1): 138-147.
Colonna-Romano, S., Arnold, W., Schlüter, A., Boistard, P., Pühler, A., and Priefer, U. B. (1990). An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK. Mol Gen Genet 223, 138-147.
Colonna-Romano, S., et al., 1990. An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK. Mol Gen Genet, 223(1), p 138-147.
S. Colonna-Romano, et al., “An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK.”, Mol Gen Genet, vol. 223, 1990, pp. 138-147.
Colonna-Romano, S., Arnold, W., Schlüter, A., Boistard, P., Pühler, A., Priefer, U.B.: An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK. Mol Gen Genet. 223, 138-147 (1990).
Colonna-Romano, S., Arnold, Walter, Schlüter, Andreas, Boistard, P., Pühler, Alfred, and Priefer, U. B. “An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK.”. Mol Gen Genet 223.1 (1990): 138-147.

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