Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum

Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Pühler A (1991)
MOLECULAR MICROBIOLOGY 5(5): 1197-1204.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in-frame overlapping genes, lysC-alpha encoding a protein of 421 amino acids (M(r) 44300) and lysC-beta encoding a protein of 172 amino acids (M(r) 18600). The C. glutamicum aspartokinase was purified and found to contain two proteins of M(r) 47 000 and M(r) 18 000. A C. glutamicum mutant expressing a feedback-resistant aspartokinase was shown to be changed in a single base pair of the lysC-beta gene, leading to an amino acid exchange in the beta-subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.
Erscheinungsjahr
1991
Zeitschriftentitel
MOLECULAR MICROBIOLOGY
Band
5
Ausgabe
5
Seite(n)
1197-1204
ISSN
0950-382X
eISSN
1365-2958
Page URI
https://pub.uni-bielefeld.de/record/1649978

Zitieren

Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Pühler A. Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY. 1991;5(5):1197-1204.
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., & Pühler, A. (1991). Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY, 5(5), 1197-1204. doi:10.1111/j.1365-2958.1991.tb01893.x
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., and Pühler, A. (1991). Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY 5, 1197-1204.
Kalinowski, J., et al., 1991. Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY, 5(5), p 1197-1204.
J. Kalinowski, et al., “Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum”, MOLECULAR MICROBIOLOGY, vol. 5, 1991, pp. 1197-1204.
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., Pühler, A.: Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY. 5, 1197-1204 (1991).
Kalinowski, Jörn, Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., and Pühler, Alfred. “Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum”. MOLECULAR MICROBIOLOGY 5.5 (1991): 1197-1204.

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PMID: 1956296
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