Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum

SCHAEFER A, Tauch A, JAEGER W, Kalinowski J, THIERBACH G, Pühler A (1994)
Gene 145(1): 69-73.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ;
Abstract / Bemerkung
Here we describe small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19. We combined the useful properties of the pK plasmids (e.g., multiple cloning site, lacZ alpha fragment, sequencing with M13 primers) with the broad-host-range transfer machinery of plasmid RP4 and a modified sacB gene from Bacillus subtilis. The new pK derivatives can be transferred by RP4-mediated conjugation into a wide range of Gram(-) and Gram(+) bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination. As an application example, the generation of a defined deletion of the hom-thrB genes in the chromosome of the Gram(+) bacterium Corynebacterium glutamicum glutamicum is presented.
Stichworte
KANAMYCIN RESISTANCE; SACB GENE; CORYNEFORM BACTERIA; GENE DISRUPTION AND REPLACEMENT; SEQUENCING VECTOR; LACZ; BROAD-HOST-RANGE CONJUGATION
Erscheinungsjahr
1994
Zeitschriftentitel
Gene
Band
145
Ausgabe
1
Seite(n)
69-73
ISSN
0378-1119
Page URI
https://pub.uni-bielefeld.de/record/1643046

Zitieren

SCHAEFER A, Tauch A, JAEGER W, Kalinowski J, THIERBACH G, Pühler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene. 1994;145(1):69-73.
SCHAEFER, A., Tauch, A., JAEGER, W., Kalinowski, J., THIERBACH, G., & Pühler, A. (1994). Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene, 145(1), 69-73. doi:10.1016/0378-1119(94)90324-7
SCHAEFER, A., Tauch, A., JAEGER, W., Kalinowski, J., THIERBACH, G., and Pühler, A. (1994). Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145, 69-73.
SCHAEFER, A., et al., 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene, 145(1), p 69-73.
A. SCHAEFER, et al., “Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum”, Gene, vol. 145, 1994, pp. 69-73.
SCHAEFER, A., Tauch, A., JAEGER, W., Kalinowski, J., THIERBACH, G., Pühler, A.: Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene. 145, 69-73 (1994).
SCHAEFER, A, Tauch, Andreas, JAEGER, W, Kalinowski, Jörn, THIERBACH, G, and Pühler, Alfred. “Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum”. Gene 145.1 (1994): 69-73.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 8045426
PubMed | Europe PMC

Suchen in

Google Scholar