Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli

Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Pühler A (1994)
FEMS MICROBIOLOGY LETTERS 123(3): 343-347.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Efficient electroporation of Escherichia coli with plasmid DNA isolated from Corynebacterium glutamicum depends on the use of Mcr-deficient E. coli strains. The transformation frequency increased nearly 800-fold when the Mcr-deficient E. coli DH5 alpha MCR was used instead of E. coli DH5 alpha. We used E. coil strains with different mutations in the methyl-specific restriction systems to show that McrBC-deficiency is sufficient to generate this effect. The results imply that C. glutamicum DNA contains methylcytosine in specific sequences recognized by the E. coli McrBC system.
Stichworte
ELECTROPORATION; MCR SYSTEM; MODIFICATION; METHYLCYTOSINE; CORYNEBACTERIUM GLUTAMICUM; RESTRICTION; MRR SYSTEM
Erscheinungsjahr
1994
Zeitschriftentitel
FEMS MICROBIOLOGY LETTERS
Band
123
Ausgabe
3
Seite(n)
343-347
ISSN
0378-1097
Page URI
https://pub.uni-bielefeld.de/record/1642272

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Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Pühler A. Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS. 1994;123(3):343-347.
Tauch, A., Kirchner, O., Wehmeier, L., Kalinowski, J., & Pühler, A. (1994). Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS, 123(3), 343-347. doi:10.1016/0378-1097(94)90216-X
Tauch, A., Kirchner, O., Wehmeier, L., Kalinowski, J., and Pühler, A. (1994). Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS 123, 343-347.
Tauch, A., et al., 1994. Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS, 123(3), p 343-347.
A. Tauch, et al., “Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli”, FEMS MICROBIOLOGY LETTERS, vol. 123, 1994, pp. 343-347.
Tauch, A., Kirchner, O., Wehmeier, L., Kalinowski, J., Pühler, A.: Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS. 123, 343-347 (1994).
Tauch, Andreas, Kirchner, O, Wehmeier, L, Kalinowski, Jörn, and Pühler, Alfred. “Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli”. FEMS MICROBIOLOGY LETTERS 123.3 (1994): 343-347.

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PMID: 7988915
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