Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation

Mateos LM, Schäfer A, Kalinowski J, Martin JF, Pühler A (1996)
JOURNAL OF BACTERIOLOGY 178(19): 5768-5775.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Conjugative transfer of mobilizable derivatives of the Escherichia coli narrow-host-range plasmids pBR322, pBR325, pACYC177, and pACYC184 from E. coli to species of the gram-positive genera Corynebacterium and Brevibacterium resulted in the integration of the plasmids into the genomes of the recipient bacteria, Transconjugants appeared at low frequencies and reproducibly with a delay of 2 to 3 days compared with matings with replicative vectors. Southern analysis of corynebacterial transconjugants and nucleotide sequences from insertion sites revealed that integration occurs at different locations and that different parts of the vector are involved in the process, Integration is not dependent on indigenous insertion sequence elements but results from recombination between very short homologous DNA segments (8 to 12 bp) present in the vector and in the host DNA. In the majority of the cases (90%), integration led to cointegrate formation, and in some cases, deletions or rearrangements occurred during the recombination event, Insertions were found to be quite stable even in the absence of selective pressure.
Erscheinungsjahr
1996
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
178
Ausgabe
19
Seite(n)
5768-5775
ISSN
0021-9193
Page URI
https://pub.uni-bielefeld.de/record/1638415

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Mateos LM, Schäfer A, Kalinowski J, Martin JF, Pühler A. Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation. JOURNAL OF BACTERIOLOGY. 1996;178(19):5768-5775.
Mateos, L. M., Schäfer, A., Kalinowski, J., Martin, J. F., & Pühler, A. (1996). Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation. JOURNAL OF BACTERIOLOGY, 178(19), 5768-5775.
Mateos, L. M., Schäfer, A., Kalinowski, J., Martin, J. F., and Pühler, A. (1996). Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation. JOURNAL OF BACTERIOLOGY 178, 5768-5775.
Mateos, L.M., et al., 1996. Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation. JOURNAL OF BACTERIOLOGY, 178(19), p 5768-5775.
L.M. Mateos, et al., “Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation”, JOURNAL OF BACTERIOLOGY, vol. 178, 1996, pp. 5768-5775.
Mateos, L.M., Schäfer, A., Kalinowski, J., Martin, J.F., Pühler, A.: Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation. JOURNAL OF BACTERIOLOGY. 178, 5768-5775 (1996).
Mateos, L.M., Schäfer, A., Kalinowski, Jörn, Martin, J. F., and Pühler, Alfred. “Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation”. JOURNAL OF BACTERIOLOGY 178.19 (1996): 5768-5775.

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