Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum

Tauch A, Zheng ZX, Pühler A, Kalinowski J (1998)
Plasmid 40(2): 126-139.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The clinical isolate Corynebacterium striatum M82B (formerly Corynebacterium xerosis M82B) carries the 50-kb R-plasmid pTP10 conferring resistance to the antibiotics chloramphenicol, erythromycin, kanamycin, and tetracycline. DNA sequence analysis of the chloramphenicol resistance region revealed the presence of the 4155-bp transposable element Tn5564. The ends of Tn5564 are identical 22-bp inverted repeats flanked by a 6-bp target site duplication. The central region of Tn5564 encodes the chloramphenicol resistance gene cmx, specifying a transmembrane chloramphenicol efflux protein, and an open reading frame homologous to transposases of insertion sequences identified in Arthrobacter nicotinovorans and Bordetella pertussis. Furthermore, the 1715-bp insertion sequence IS1513 encoding a putative transposase of the IS30 family is an integral part of Tn5564 and is located upstream of cmx For transposon mutagenesis, Tn5564 was transferred to Corynebacterium glutamicum on a mobilizable Escherichia coli plasmid using RP4-mediated intergeneric conjugation. Transposition of Tn5564 in C. glutamicum occurred with a frequency of 3.3 X 10(-8) and resulted in an insertion into target sites containing the central palindromic tetranucleotide CTAG. A Tn5564-induced mutant strain of C. glutamicum was found to carry the transposon in the ftsZ gene region. (C) 1998 Academic Press.
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Tauch A, Zheng ZX, Pühler A, Kalinowski J. Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid. 1998;40(2):126-139.
Tauch, A., Zheng, Z. X., Pühler, A., & Kalinowski, J. (1998). Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid, 40(2), 126-139.
Tauch, A., Zheng, Z. X., Pühler, A., and Kalinowski, J. (1998). Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid 40, 126-139.
Tauch, A., et al., 1998. Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid, 40(2), p 126-139.
A. Tauch, et al., “Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum”, Plasmid, vol. 40, 1998, pp. 126-139.
Tauch, A., Zheng, Z.X., Pühler, A., Kalinowski, J.: Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid. 40, 126-139 (1998).
Tauch, Andreas, Zheng, ZX, Pühler, Alfred, and Kalinowski, Jörn. “Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum”. Plasmid 40.2 (1998): 126-139.

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