The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism

Quast K, Bathe B, Pühler A, Kalinowski J (1999)
Mol Gen Genet 262(3): 568-578.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
An IS element, termed ISCg2, was identified in the chromosome of Corynebacterium glutamicum ATCC 13032. After screening a cosmid library of the C. glutamicum ATCC 13032 genome, six copies of ISCg2 including their flanking regions were sequenced and analyzed. ISCg2 is 1636 bp in length and has 26-bp imperfect inverted repeats flanked by 3-bp direct repeats. By comparisons with other IS elements,ISCg2 was classified as a member of the IS30 family of insertion sequences. The six copies of ISCg2 were identical at the nucleotide level and were located in intergenic, AT-rich regions of the chromosome. The regions in which the six copies of ISCg2 were inserted displayed significant similarities. This similarity extends over a region of 65 bp, which was assumed to be the target region for ISCg2. Interestingly, live of the six copies of ISCg2 were located adjacent to genes that may be involved in aspartate and glutamate metabolism or its regulation. Investigation of the distribution of ISCg2 showed that the IS element is restricted to certain C. glutamicum strains. Analysis of various integration regions indicates active transposition of ISCg2. in C. glutamicum.
Stichworte
Corynebacterium; nitrogen; insertion element; metabolism; target specificity
Erscheinungsjahr
1999
Zeitschriftentitel
Mol Gen Genet
Band
262
Ausgabe
3
Seite(n)
568-578
ISSN
0026-8925
eISSN
1432-1874
Page URI
https://pub.uni-bielefeld.de/record/1621156

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Quast K, Bathe B, Pühler A, Kalinowski J. The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet. 1999;262(3):568-578.
Quast, K., Bathe, B., Pühler, A., & Kalinowski, J. (1999). The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet, 262(3), 568-578. doi:10.1007/s004380051119
Quast, K., Bathe, B., Pühler, A., and Kalinowski, J. (1999). The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet 262, 568-578.
Quast, K., et al., 1999. The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet, 262(3), p 568-578.
K. Quast, et al., “The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism”, Mol Gen Genet, vol. 262, 1999, pp. 568-578.
Quast, K., Bathe, B., Pühler, A., Kalinowski, J.: The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet. 262, 568-578 (1999).
Quast, K, Bathe, B, Pühler, Alfred, and Kalinowski, Jörn. “The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism”. Mol Gen Genet 262.3 (1999): 568-578.

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