The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism
Quast K, Bathe B, Pühler A, Kalinowski J (1999)
Mol Gen Genet 262(3): 568-578.
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Einrichtung
Abstract / Bemerkung
An IS element, termed ISCg2, was identified in the chromosome of Corynebacterium glutamicum ATCC 13032. After screening a cosmid library of the C. glutamicum ATCC 13032 genome, six copies of ISCg2 including their flanking regions were sequenced and analyzed. ISCg2 is 1636 bp in length and has 26-bp imperfect inverted repeats flanked by 3-bp direct repeats. By comparisons with other IS elements,ISCg2 was classified as a member of the IS30 family of insertion sequences. The six copies of ISCg2 were identical at the nucleotide level and were located in intergenic, AT-rich regions of the chromosome. The regions in which the six copies of ISCg2 were inserted displayed significant similarities. This similarity extends over a region of 65 bp, which was assumed to be the target region for ISCg2. Interestingly, live of the six copies of ISCg2 were located adjacent to genes that may be involved in aspartate and glutamate metabolism or its regulation. Investigation of the distribution of ISCg2 showed that the IS element is restricted to certain C. glutamicum strains. Analysis of various integration regions indicates active transposition of ISCg2. in C. glutamicum.
Stichworte
Corynebacterium;
nitrogen;
insertion element;
metabolism;
target specificity
Erscheinungsjahr
1999
Zeitschriftentitel
Mol Gen Genet
Band
262
Ausgabe
3
Seite(n)
568-578
ISSN
0026-8925
eISSN
1432-1874
Page URI
https://pub.uni-bielefeld.de/record/1621156
Zitieren
Quast K, Bathe B, Pühler A, Kalinowski J. The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet. 1999;262(3):568-578.
Quast, K., Bathe, B., Pühler, A., & Kalinowski, J. (1999). The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet, 262(3), 568-578. https://doi.org/10.1007/s004380051119
Quast, K, Bathe, B, Pühler, Alfred, and Kalinowski, Jörn. 1999. “The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism”. Mol Gen Genet 262 (3): 568-578.
Quast, K., Bathe, B., Pühler, A., and Kalinowski, J. (1999). The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet 262, 568-578.
Quast, K., et al., 1999. The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet, 262(3), p 568-578.
K. Quast, et al., “The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism”, Mol Gen Genet, vol. 262, 1999, pp. 568-578.
Quast, K., Bathe, B., Pühler, A., Kalinowski, J.: The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet. 262, 568-578 (1999).
Quast, K, Bathe, B, Pühler, Alfred, and Kalinowski, Jörn. “The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism”. Mol Gen Genet 262.3 (1999): 568-578.
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8 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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Transposition and target specificity of the typical IS30 family element IS1655 from Neisseria meningitidis.
Kiss J, Nagy Z, Tóth G, Kiss GB, Jakab J, Chandler M, Olasz F., Mol Microbiol 63(6), 2007
PMID: 17367392
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Barreiro C, González-Lavado E, Brand S, Tauch A, Martín JF., J Bacteriol 187(3), 2005
PMID: 15659666
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Vertès AA, Inui M, Yukawa H., Appl Environ Microbiol 71(12), 2005
PMID: 16332735
Vertès AA, Inui M, Yukawa H., Appl Environ Microbiol 71(12), 2005
PMID: 16332735
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A., J Biotechnol 104(1-3), 2003
PMID: 12948626
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A., J Biotechnol 104(1-3), 2003
PMID: 12948626
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Pátek M, Muth G, Wohlleben W., J Biotechnol 104(1-3), 2003
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Pátek M, Muth G, Wohlleben W., J Biotechnol 104(1-3), 2003
PMID: 12948649
Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library.
Tauch A, Homann I, Mormann S, Rüberg S, Billault A, Bathe B, Brand S, Brockmann-Gretza O, Rückert C, Schischka N, Wrenger C, Hoheisel J, Möckel B, Huthmacher K, Pfefferle W, Pühler A, Kalinowski J., J Biotechnol 95(1), 2002
PMID: 11879709
Tauch A, Homann I, Mormann S, Rüberg S, Billault A, Bathe B, Brand S, Brockmann-Gretza O, Rückert C, Schischka N, Wrenger C, Hoheisel J, Möckel B, Huthmacher K, Pfefferle W, Pühler A, Kalinowski J., J Biotechnol 95(1), 2002
PMID: 11879709
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