Proteome analysis of Corynebacterium glutamicum

Hermann T, Pfefferle W, Baumann C, Busker E, Schaffer S, Bott M, Sahm H, Dusch N, Kalinowski J, Pühler A, Bendt AK, et al. (2001)
ELECTROPHORESIS 22(9): 1712-1723.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Hermann, T.; Pfefferle, W.; Baumann, C.; Busker, E.; Schaffer, S.; Bott, M.; Sahm, H.; Dusch, N.; Kalinowski, JörnUniBi; Pühler, AlfredUniBi ; Bendt, A. K.; Kramer, R.
Alle
Einrichtung
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Fakultät für Biologie
Abstract / Bemerkung
By the use of different Corynebacterium glutamicum strains move than 1.4 million tons of amino acids, mainly L-glutamate and L-lysine, are produced per year. A project was started recently to elucidate the complete DNA sequence of this bacterium. In this communication we describe an approach to analyze the C, glutamicum proteome, based on this genetic information, by a combination of two-dimensional (2-D) gel electrophoresis and protein identification via microsequencing or mass spectrometry. We used these techniques to resolve proteins of C, glutamicum with the aim to establish 2-D protein maps as a tool for basic microbiology and for strain improvement. In order to analyze the C. glutamicum proteome, methods were established to fractionate the C. glutamicum proteins according to functional entities, i.e., cytoplasm, membranes, and cell wall. Protein spots of the cytoplasmic and membrane fraction were identified by N-terminal sequencing, immunodetection, matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electrospray ionization-mass spectrometry (ESI-MS). Additionally, a protocol to analyze proteins secreted by C. glutamicum was established. Approximately 40 protein spots were observed on silver-stained 2-D gels, 12 of which were identified.
Stichworte
Corynebacterium glutamicum; two-dimensional electrophoresis; Mycobacterium; proteome
Erscheinungsjahr
2001
Zeitschriftentitel
ELECTROPHORESIS
Band
22
Ausgabe
9
Seite(n)
1712-1723
ISSN
0173-0835
eISSN
1522-2683
Page URI
https://pub.uni-bielefeld.de/record/1616981

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Hermann T, Pfefferle W, Baumann C, et al. Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS. 2001;22(9):1712-1723.
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., et al. (2001). Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS, 22(9), 1712-1723. https://doi.org/10.1002/1522-2683(200105)22:9<1712::AID-ELPS1712>3.0.CO;2-G
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., et al. 2001. “Proteome analysis of Corynebacterium glutamicum”. ELECTROPHORESIS 22 (9): 1712-1723.
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., Dusch, N., Kalinowski, J., Pühler, A., et al. (2001). Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS 22, 1712-1723.
Hermann, T., et al., 2001. Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS, 22(9), p 1712-1723.
T. Hermann, et al., “Proteome analysis of Corynebacterium glutamicum”, ELECTROPHORESIS, vol. 22, 2001, pp. 1712-1723.
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., Dusch, N., Kalinowski, J., Pühler, A., Bendt, A.K., Kramer, R., Burkovski, A.: Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS. 22, 1712-1723 (2001).
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., Dusch, N., Kalinowski, Jörn, Pühler, Alfred, Bendt, A. K., Kramer, R., and Burkovski, A. “Proteome analysis of Corynebacterium glutamicum”. ELECTROPHORESIS 22.9 (2001): 1712-1723.

52 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Effects of EGTA on cell surface structures of Corynebacterium glutamicum.
Theresia NM, Aida K, Takada A, Iwai N, Wachi M., Arch Microbiol 200(2), 2018
PMID: 29075867
Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome.
Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ, Krylov AA, Mashko SV., Appl Microbiol Biotechnol 102(6), 2018
PMID: 29392386
Metabolic engineering of Corynebacterium glutamicum for fermentative production of chemicals in biorefinery.
Baritugo KA, Kim HT, David Y, Choi JI, Hong SH, Jeong KJ, Choi JH, Joo JC, Park SJ., Appl Microbiol Biotechnol 102(9), 2018
PMID: 29557518
Development of a Novel Gene Expression System for Secretory Production of Heterologous Proteins via the General Secretory (Sec) Pathway in Corynebacterium Glutamicum.
Jia H, Li H, Zhang L, Xu D., Iran J Biotechnol 16(1), 2018
PMID: 30555839
Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette.
Huang Y, Li L, Xie S, Zhao N, Han S, Lin Y, Zheng S., Sci Rep 7(1), 2017
PMID: 28801604
Synthetic biology for manufacturing chemicals: constraints drive the use of non-conventional microbial platforms.
Czajka J, Wang Q, Wang Y, Tang YJ., Appl Microbiol Biotechnol 101(20), 2017
PMID: 28884354
Development of a genetically engineered Escherichia coli strain for plasmid transformation in Corynebacterium glutamicum.
Li H, Zhang L, Guo W, Xu D., J Microbiol Methods 131(), 2016
PMID: 27793586
Development of a secretion system for the production of heterologous proteins in Corynebacterium glutamicum using the Porin B signal peptide.
An SJ, Yim SS, Jeong KJ., Protein Expr Purif 89(2), 2013
PMID: 23597779
The ppm operon is essential for acylation and glycosylation of lipoproteins in Corynebacterium glutamicum.
Mohiman N, Argentini M, Batt SM, Cornu D, Masi M, Eggeling L, Besra G, Bayan N., PLoS One 7(9), 2012
PMID: 23029442
Tools for genetic manipulations in Corynebacterium glutamicum and their applications.
Nešvera J, Pátek M., Appl Microbiol Biotechnol 90(5), 2011
PMID: 21519933
High yield secretion of heterologous proteins in Corynebacterium glutamicum using its own Tat-type signal sequence.
Teramoto H, Watanabe K, Suzuki N, Inui M, Yukawa H., Appl Microbiol Biotechnol 91(3), 2011
PMID: 21523478
Proteomics of corynebacteria: From biotechnology workhorses to pathogens.
Poetsch A, Haussmann U, Burkovski A., Proteomics 11(15), 2011
PMID: 21674800
A 2D reversed-phase x ion-pair reversed-phase HPLC-MALDI TOF/TOF-MS approach for shotgun proteome analysis.
Lasaosa M, Delmotte N, Huber CG, Melchior K, Heinzle E, Tholey A., Anal Bioanal Chem 393(4), 2009
PMID: 19066860
Identification of new secreted proteins and secretion of heterologous amylase by C. glutamicum.
Suzuki N, Watanabe K, Okibe N, Tsuchida Y, Inui M, Yukawa H., Appl Microbiol Biotechnol 82(3), 2009
PMID: 19066885
Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum.
Barreiro C, Nakunst D, Hüser AT, de Paz HD, Kalinowski J, Martín JF., Microbiology 155(pt 2), 2009
PMID: 19202085
A fractionation procedure for identifying novel proteins induced by chill stress in Arabidopsis thaliana.
Sun L, Wang S, Xi J, Yang S, Liu X, Chai X, Xin H, An B, Hao D., Biotechnol Lett 31(8), 2009
PMID: 19347259
Cell growth and cell division in the rod-shaped actinomycete Corynebacterium glutamicum.
Letek M, Fiuza M, Ordóñez E, Villadangos AF, Ramos A, Mateos LM, Gil JA., Antonie Van Leeuwenhoek 94(1), 2008
PMID: 18283557
Selectivity of bacterial proteome fractionation based on differential solubility: a mass spectrometry evaluation.
Ramos Y, García Y, Llopiz A, Castellanos-Serra L., Anal Biochem 377(2), 2008
PMID: 18384741
Application of proteomics in biotechnology--microbial proteomics.
Josic D, Kovac S., Biotechnol J 3(4), 2008
PMID: 18320565
Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes.
Barriuso-Iglesias M, Schluesener D, Barreiro C, Poetsch A, Martín JF., BMC Microbiol 8(), 2008
PMID: 19091079
Comparative proteomes of Corynebacterium glutamicum grown on aromatic compounds revealed novel proteins involved in aromatic degradation and a clear link between aromatic catabolism and gluconeogenesis via fructose-1,6-bisphosphatase.
Qi SW, Chaudhry MT, Zhang Y, Meng B, Huang Y, Zhao KX, Poetsch A, Jiang CY, Liu S, Liu SJ., Proteomics 7(20), 2007
PMID: 17880007
Cytoplasmic proteome reference map for a glutamic acid-producing Corynebacterium glutamicum ATCC 14067.
Li L, Wada M, Yokota A., Proteomics 7(23), 2007
PMID: 18040983
Toward the complete membrane proteome: high coverage of integral membrane proteins through transmembrane peptide detection.
Fischer F, Wolters D, Rögner M, Poetsch A., Mol Cell Proteomics 5(3), 2006
PMID: 16291997
Emerging Corynebacterium glutamicum systems biology.
Wendisch VF, Bott M, Kalinowski J, Oldiges M, Wiechert W., J Biotechnol 124(1), 2006
PMID: 16406159
Application of global analysis techniques to Corynebacterium glutamicum: new insights into nitrogen regulation.
Silberbach M, Burkovski A., J Biotechnol 126(1), 2006
PMID: 16698104
Single-gene knockout of a novel regulatory element confers ethionine resistance and elevates methionine production in Corynebacterium glutamicum.
Mampel J, Schröder H, Haefner S, Sauer U., Appl Microbiol Biotechnol 68(2), 2005
PMID: 15668756
Heat shock proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone.
Barreiro C, González-Lavado E, Brand S, Tauch A, Martín JF., J Bacteriol 187(3), 2005
PMID: 15659666
Gene expression systems in corynebacteria.
Srivastava P, Deb JK., Protein Expr Purif 40(2), 2005
PMID: 15766862
Mapping the membrane proteome of Corynebacterium glutamicum.
Schluesener D, Fischer F, Kruip J, Rögner M, Poetsch A., Proteomics 5(5), 2005
PMID: 15717325
Changes in the extracellular proteome caused by the absence of the bldA gene product, a developmentally significant tRNA, reveal a new target for the pleiotropic regulator AdpA in Streptomyces coelicolor.
Kim DW, Chater K, Lee KJ, Hesketh A., J Bacteriol 187(9), 2005
PMID: 15838021
Towards the proteome of the marine bacterium Rhodopirellula baltica: mapping the soluble proteins.
Gade D, Theiss D, Lange D, Mirgorodskaya E, Lombardot T, Glöckner FO, Kube M, Reinhardt R, Amann R, Lehrach H, Rabus R, Gobom J., Proteomics 5(14), 2005
PMID: 16127728
Regulation of AmtR-controlled gene expression in Corynebacterium glutamicum: mechanism and characterization of the AmtR regulon.
Beckers G, Strösser J, Hildebrandt U, Kalinowski J, Farwick M, Krämer R, Burkovski A., Mol Microbiol 58(2), 2005
PMID: 16194241
The transcriptional regulator SsuR activates expression of the Corynebacterium glutamicum sulphonate utilization genes in the absence of sulphate.
Koch DJ, Rückert C, Albersmeier A, Hüser AT, Tauch A, Pühler A, Kalinowski J., Mol Microbiol 58(2), 2005
PMID: 16194234
Assessing the precision of high-throughput computational and laboratory approaches for the genome-wide identification of protein subcellular localization in bacteria.
Rey S, Gardy JL, Brinkman FS., BMC Genomics 6(), 2005
PMID: 16288665
Manipulating corynebacteria, from individual genes to chromosomes.
Vertès AA, Inui M, Yukawa H., Appl Environ Microbiol 71(12), 2005
PMID: 16332735
In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome.
Krömer JO, Sorgenfrei O, Klopprogge K, Heinzle E, Wittmann C., J Bacteriol 186(6), 2004
PMID: 14996808
Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy.
Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Pühler A, Kalinowski J., J Biotechnol 112(1-2), 2004
PMID: 15288952
The glycosylated cell surface protein Rpf2, containing a resuscitation-promoting factor motif, is involved in intercellular communication of Corynebacterium glutamicum.
Hartmann M, Barsch A, Niehaus K, Pühler A, Tauch A, Kalinowski J., Arch Microbiol 182(4), 2004
PMID: 15480574
Regulation of GlnK activity: modification, membrane sequestration and proteolysis as regulatory principles in the network of nitrogen control in Corynebacterium glutamicum.
Strösser J, Lüdke A, Schaffer S, Krämer R, Burkovski A., Mol Microbiol 54(1), 2004
PMID: 15458411
Using functional genomics to improve productivity in the manufacture of industrial biochemicals.
Hermann T., Curr Opin Biotechnol 15(5), 2004
PMID: 15464376
Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum.
Beckers G, Bendt AK, Krämer R, Burkovski A., J Bacteriol 186(22), 2004
PMID: 15516578
Mass spectrometric approaches for characterizing bacterial proteomes.
VerBerkmoes NC, Connelly HM, Pan C, Hettich RL., Expert Rev Proteomics 1(4), 2004
PMID: 15966840
The putative transcriptional repressor McbR, member of the TetR-family, is involved in the regulation of the metabolic network directing the synthesis of sulfur containing amino acids in Corynebacterium glutamicum.
Rey DA, Pühler A, Kalinowski J., J Biotechnol 103(1), 2003
PMID: 12770504
Mycomembrane and S-layer: two important structures of Corynebacterium glutamicum cell envelope with promising biotechnology applications.
Bayan N, Houssin C, Chami M, Leblon G., J Biotechnol 104(1-3), 2003
PMID: 12948629
Acetate metabolism and its regulation in Corynebacterium glutamicum.
Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ., J Biotechnol 104(1-3), 2003
PMID: 12948633
Genome-wide analysis of the L-methionine biosynthetic pathway in Corynebacterium glutamicum by targeted gene deletion and homologous complementation.
Rückert C, Pühler A, Kalinowski J., J Biotechnol 104(1-3), 2003
PMID: 12948640
Genome-wide expression analysis in Corynebacterium glutamicum using DNA microarrays.
Wendisch VF., J Biotechnol 104(1-3), 2003
PMID: 12948645
Ammonium assimilation and nitrogen control in Corynebacterium glutamicum and its relatives: an example for new regulatory mechanisms in actinomycetes.
Burkovski A., FEMS Microbiol Rev 27(5), 2003
PMID: 14638415
Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle.
Claes WA, Pühler A, Kalinowski J., J Bacteriol 184(10), 2002
PMID: 11976302
The use of enzymes in the chemical industry in Europe.
Schmid A, Hollmann F, Park JB, Bühler B., Curr Opin Biotechnol 13(4), 2002
PMID: 12323359
A high-resolution reference map for cytoplasmic and membrane-associated proteins of Corynebacterium glutamicum.
Schaffer S, Weil B, Nguyen VD, Dongmann G, Günther K, Nickolaus M, Hermann T, Bott M., Electrophoresis 22(20), 2001
PMID: 11824608
Sensing nitrogen limitation in Corynebacterium glutamicum: the role of glnK and glnD.
Nolden L, Ngouoto-Nkili CE, Bendt AK, Krämer R, Burkovski A., Mol Microbiol 42(5), 2001
PMID: 11886559

30 References

Daten bereitgestellt von Europe PubMed Central.


Leuchtenberger, 1996
Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences.
Pascual C, Lawson PA, Farrow JA, Gimenez MN, Collins MD., Int. J. Syst. Bacteriol. 45(4), 1995
PMID: 7547291
LION and Degussa apply genomics to fermentation.
Hodgson J., Nat. Biotechnol. 16(8), 1998
PMID: 9702763
Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon.
Keilhauer C, Eggeling L, Sahm H., J. Bacteriol. 175(17), 1993
PMID: 8366043
Mapping and identification of Corynebacterium glutamicum proteins by two-dimensional gel electrophoresis and microsequencing.
Hermann T, Wersch G, Uhlemann EM, Schmid R, Burkovski A., Electrophoresis 19(18), 1998
PMID: 9932818
Two-dimensional electrophoretic analysis of Corynebacterium glutamicum membrane fraction and surface proteins.
Hermann T, Finkemeier M, Pfefferle W, Wersch G, Kramer R, Burkovski A., Electrophoresis 21(3), 2000
PMID: 10726773
Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis.
Molloy MP, Herbert BR, Walsh BJ, Tyler MI, Traini M, Sanchez JC, Hochstrasser DF, Williams KL, Gooley AA., Electrophoresis 19(5), 1998
PMID: 9629924
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.
Neuhoff V, Arold N, Taube D, Ehrhardt W., Electrophoresis 9(6), 1988
PMID: 2466658

Blum, Electrophoresis 8(), 1987
Response to nitrogen starvation in Corynebacterium glutamicum.
Schmi R, Uhlemann EM, Nolden L, Wersch G, Hecker R, Hermann T, Marx A, Burkovski A., FEMS Microbiol. Lett. 187(1), 2000
PMID: 10828405
An agarose-based gel-concentration system for microsequence and mass spectrometric characterization of proteins previously purified in polyacrylamide gels starting at low picomole levels.
Rider MH, Puype M, Van Damme J, Gevaert K, De Boeck S, D'Alayer J, Rasmussen HH, Celis JE, Vanderkerchove J., Eur. J. Biochem. 230(1), 1995
PMID: 7601109
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
Eng JK, McCormack AL, Yates JR., J. Am. Soc. Mass Spectrom. 5(11), 1994
PMID: c6781
Ultrastructure of the Corynebacterium glutamicum cell wall.
Marienfeld S, Uhlemann EM, Schmid R, Kramer R, Burkovski A., Antonie Van Leeuwenhoek 72(4), 1997
PMID: 9442270
A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome.
Bathe B, Kalinowski J, Puhler A., Mol. Gen. Genet. 252(3), 1996
PMID: 8842145
Mapping and identification of Mycobacterium tuberculosis proteins by two-dimensional gel electrophoresis, microsequencing and immunodetection.
Rosenkrands I, Weldingh K, Jacobsen S, Hansen CV, Florio W, Gianetri I, Andersen P., Electrophoresis 21(5), 2000
PMID: 10768780
Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae.
Himmelreich R, Hilbert H, Plagens H, Pirkl E, Li BC, Herrmann R., Nucleic Acids Res. 24(22), 1996
PMID: 8948633
Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Link AJ, Robison K, Church GM., Electrophoresis 18(8), 1997
PMID: 9298646
Diagnosis of cellular states of microbial organisms using proteomics.
VanBogelen RA, Schiller EE, Thomas JD, Neidhardt FC., Electrophoresis 20(11), 1999
PMID: 10493120
Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins.
Gorg A, Obermaier C, Boguth G, Csordas A, Diaz JJ, Madjar JJ., Electrophoresis 18(3-4), 1997
PMID: 9150910
Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient.
Gorg A, Boguth G, Obermaier C, Weiss W., Electrophoresis 19(8-9), 1998
PMID: 9694305
Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: wide pH gradients up to pH 12, longer separation distances and simplified procedures.
Gorg A, Obermaier C, Boguth G, Weiss W., Electrophoresis 20(4-5), 1999
PMID: 10344237
The current state of two-dimensional electrophoresis with immobilized pH gradients.
Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W., Electrophoresis 21(6), 2000
PMID: 10786879
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
Nielsen H, Engelbrecht J, Brunak S, von Heijne G., Protein Eng. 10(1), 1997
PMID: 9051728
Proposal for a common nomenclature for sequence ions in mass spectra of peptides.
Roepstorff P, Fohlman J., Biomed. Mass Spectrom. 11(11), 1984
PMID: 6525415
Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I.
Jakoby M, Tesch M, Sahm H, Kramer R, Burkovski A., FEMS Microbiol. Lett. 154(1), 1997
PMID: 9297824
Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins.
Jakoby M, Kramer R, Burkovski A., FEMS Microbiol. Lett. 173(2), 1999
PMID: 10227160
Cyano2Dbase updated: linkage of 234 protein spots to corresponding genes through N-terminal microsequencing.
Sazuka T, Yamaguchi M, Ohara O., Electrophoresis 20(11), 1999
PMID: 10493121
Two-dimensional electrophoresis of membrane proteins: a current challenge for immobilized pH gradients.
Adessi C, Miege C, Albrieux C, Rabilloud T., Electrophoresis 18(1), 1997
PMID: 9059834
Low molecular weight proteins: a challenge for post-genomic research.
Rudd KE, Humphery-Smith I, Wasinger VC, Bairoch A., Electrophoresis 19(4), 1998
PMID: 9588799
Two-dimensional gel electrophoresis for proteome projects: the effects of protein hydrophobicity and copy number.
Wilkins MR, Gasteiger E, Sanchez JC, Bairoch A, Hochstrasser DF., Electrophoresis 19(8-9), 1998
PMID: 9694302
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