Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core

Vorhölter F-J, Niehaus K, Pühler A (2001)
MOLECULAR GENETICS AND GENOMICS 266(1): 79-95.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
As a result of mutational and DNA sequence analysis, a wxc gene cluster involved in the synthesis of the surface lipopolysaccharide (LPS) was identified in Xanthomonas campestris pv. campestris. This gene cluster comprises 15 genes. It was located on a cloned 35-kb fragment of chromosomal DNA, close, but not directly adjacent, to previously characterized genes for LPS biosynthesis. The G + C content of all but one of the it we genes was atypically low for X. campestris pv. campestris, while the G + C distribution was uniform throughout the cluster. An SDS-PAGE analysis of mutant strains defective in various wxc genes confirmed that genes from this cluster were involved in LPS biosynthesis. The mutant phenotypes allowed the differentiation of three regions within the wxc cluster. Genes from wxc region 1 are necessary for the biosynthesis of the water-soluble LPS O-antigen. Analysis of DNA and deduced amino acid sequences led to the identification of two glycosyltransferases, two components of an ABC transport system, and a possible kinase among the seven putative proteins encoded by genes constituting wxc region 1. The two genes in wxc region 2 were similar to gmd and rmd, which direct the synthesis of the sugar nucleotide GDP-D-rhamnose. Mutations affecting wxc region 2 demonstrated its involvement in the formation of the LPS core. Genes from wxc region 3 showed similarities to genes that code for enzymes that modify nucleotide sugars, and to components of sugar translocation systems that have so far been rarely described in bacteria.
Erscheinungsjahr
Zeitschriftentitel
MOLECULAR GENETICS AND GENOMICS
Band
266
Ausgabe
1
Seite(n)
79-95
ISSN
eISSN
PUB-ID

Zitieren

Vorhölter F-J, Niehaus K, Pühler A. Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core. MOLECULAR GENETICS AND GENOMICS. 2001;266(1):79-95.
Vorhölter, F. - J., Niehaus, K., & Pühler, A. (2001). Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core. MOLECULAR GENETICS AND GENOMICS, 266(1), 79-95. doi:10.1007/s004380100521
Vorhölter, F. - J., Niehaus, K., and Pühler, A. (2001). Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core. MOLECULAR GENETICS AND GENOMICS 266, 79-95.
Vorhölter, F.-J., Niehaus, K., & Pühler, A., 2001. Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core. MOLECULAR GENETICS AND GENOMICS, 266(1), p 79-95.
F.-J. Vorhölter, K. Niehaus, and A. Pühler, “Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core”, MOLECULAR GENETICS AND GENOMICS, vol. 266, 2001, pp. 79-95.
Vorhölter, F.-J., Niehaus, K., Pühler, A.: Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core. MOLECULAR GENETICS AND GENOMICS. 266, 79-95 (2001).
Vorhölter, Frank-Jörg, Niehaus, Karsten, and Pühler, Alfred. “Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core”. MOLECULAR GENETICS AND GENOMICS 266.1 (2001): 79-95.

46 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

WxcX is involved in bacterial attachment and virulence in Xanthomonas campestris pv. campestris.
Liu YF, Liao CT, Chiang YC, Li CE, Hsiao YM., J Basic Microbiol 58(5), 2018
PMID: 29504631
Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase.
Alkhateeb RS, Vorhölter FJ, Steffens T, Rückert C, Ortseifen V, Hublik G, Niehaus K, Pühler A., Appl Microbiol Biotechnol 102(15), 2018
PMID: 29858955
Genomic Inference of Recombination-Mediated Evolution in Xanthomonas euvesicatoria and X. perforans.
Jibrin MO, Potnis N, Timilsina S, Minsavage GV, Vallad GE, Roberts PD, Jones JB, Goss EM., Appl Environ Microbiol 84(13), 2018
PMID: 29678917
Comparative genomics of host adaptive traits in Xanthomonas translucens pv. graminis.
Hersemann L, Wibberg D, Blom J, Goesmann A, Widmer F, Vorhölter FJ, Kölliker R., BMC Genomics 18(1), 2017
PMID: 28056815
Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris.
Schatschneider S, Schneider J, Blom J, Létisse F, Niehaus K, Goesmann A, Vorhölter FJ., Microbiology 163(8), 2017
PMID: 28795660
The influence of a modified lipopolysaccharide O-antigen on the biosynthesis of xanthan in Xanthomonas campestris pv. campestris B100.
Steffens T, Vorhölter FJ, Giampà M, Hublik G, Pühler A, Niehaus K., BMC Microbiol 16(), 2016
PMID: 27215401
Role of Glycosyltransferases Modifying Type B Flagellin of Emerging Hypervirulent Clostridium difficile Lineages and Their Impact on Motility and Biofilm Formation.
Valiente E, Bouché L, Hitchen P, Faulds-Pain A, Songane M, Dawson LF, Donahue E, Stabler RA, Panico M, Morris HR, Bajaj-Elliott M, Logan SM, Dell A, Wren BW., J Biol Chem 291(49), 2016
PMID: 27703012
Full Genome Sequence Analysis of Two Isolates Reveals a Novel Xanthomonas Species Close to the Sugarcane Pathogen Xanthomonas albilineans.
Pieretti I, Cociancich S, Bolot S, Carrère S, Morisset A, Rott P, Royer M., Genes (Basel) 6(3), 2015
PMID: 26213974
Establishment, in silico analysis, and experimental verification of a large-scale metabolic network of the xanthan producing Xanthomonas campestris pv. campestris strain B100.
Schatschneider S, Persicke M, Watt SA, Hublik G, Pühler A, Niehaus K, Vorhölter FJ., J Biotechnol 167(2), 2013
PMID: 23395674
Dynamic protein phosphorylation during the growth of Xanthomonas campestris pv. campestris B100 revealed by a gel-based proteomics approach.
Musa YR, Bäsell K, Schatschneider S, Vorhölter FJ, Becher D, Niehaus K., J Biotechnol 167(2), 2013
PMID: 23792782
Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper.
Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol M, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB., BMC Genomics 12(), 2011
PMID: 21396108
Structural analysis and involvement in plant innate immunity of Xanthomonas axonopodis pv. citri lipopolysaccharide.
Casabuono A, Petrocelli S, Ottado J, Orellano EG, Couto AS., J Biol Chem 286(29), 2011
PMID: 21596742
Regulation and secretion of Xanthomonas virulence factors.
Büttner D, Bonas U., FEMS Microbiol Rev 34(2), 2010
PMID: 19925633
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii.
Moreira LM, Almeida NF, Potnis N, Digiampietri LA, Adi SS, Bortolossi JC, da Silva AC, da Silva AM, de Moraes FE, de Oliveira JC, de Souza RF, Facincani AP, Ferraz AL, Ferro MI, Furlan LR, Gimenez DF, Jones JB, Kitajima EW, Laia ML, Leite RP, Nishiyama MY, Rodrigues Neto J, Nociti LA, Norman DJ, Ostroski EH, Pereira HA, Staskawicz BJ, Tezza RI, Ferro JA, Vinatzer BA, Setubal JC., BMC Genomics 11(), 2010
PMID: 20388224
The Azospirillum brasilense Sp7 noeJ and noeL genes are involved in extracellular polysaccharide biosynthesis.
Lerner A, Castro-Sowinski S, Valverde A, Lerner H, Dror R, Okon Y, Burdman S., Microbiology 155(pt 12), 2009
PMID: 19762447
The Xanthomonas oryzae pv. oryzae PhoPQ two-component system is required for AvrXA21 activity, hrpG expression, and virulence.
Lee SW, Jeong KS, Han SW, Lee SE, Phee BK, Hahn TR, Ronald P., J Bacteriol 190(6), 2008
PMID: 18203830
The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis.
Vorhölter FJ, Schneiker S, Goesmann A, Krause L, Bekel T, Kaiser O, Linke B, Patschkowski T, Rückert C, Schmid J, Sidhu VK, Sieber V, Tauch A, Watt SA, Weisshaar B, Becker A, Niehaus K, Pühler A., J Biotechnol 134(1-2), 2008
PMID: 18304669
Acquisition and evolution of plant pathogenesis-associated gene clusters and candidate determinants of tissue-specificity in xanthomonas.
Lu H, Patil P, Van Sluys MA, White FF, Ryan RP, Dow JM, Rabinowicz P, Salzberg SL, Leach JE, Sonti R, Brendel V, Bogdanove AJ., PLoS One 3(11), 2008
PMID: 19043590
Comparative and functional genomics reveals genetic diversity and determinants of host specificity among reference strains and a large collection of Chinese isolates of the phytopathogen Xanthomonas campestris pv. campestris.
He YQ, Zhang L, Jiang BL, Zhang ZC, Xu RQ, Tang DJ, Qin J, Jiang W, Zhang X, Liao J, Cao JR, Zhang SS, Wei ML, Liang XX, Lu GT, Feng JX, Chen B, Cheng J, Tang JL., Genome Biol 8(10), 2007
PMID: 17927820
Comparative analyses of Xanthomonas and Xylella complete genomes.
Moreira LM, De Souza RF, Digiampietri LA, Da Silva AC, Setubal JC., OMICS 9(1), 2005
PMID: 15805778
The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice.
Lee BM, Park YJ, Park DS, Kang HW, Kim JG, Song ES, Park IC, Yoon UH, Hahn JH, Koo BS, Lee GB, Kim H, Park HS, Yoon KO, Kim JH, Jung CH, Koh NH, Seo JS, Go SJ., Nucleic Acids Res 33(2), 2005
PMID: 15673718
Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence.
Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klösgen U, Patschkowski T, Rückert C, Rupp O, Schneiker S, Schuster SC, Vorhölter FJ, Weber E, Pühler A, Bonas U, Bartels D, Kaiser O., J Bacteriol 187(21), 2005
PMID: 16237009
Insights into the genomic basis of niche specificity of Pseudomonas putida KT2440.
Dos Santos VA, Heim S, Moore ER, Strätz M, Timmis KN., Environ Microbiol 6(12), 2004
PMID: 15560824
Comparison of two Xanthomonas campestris pathovar campestris genomes revealed differences in their gene composition.
Vorhölter FJ, Thias T, Meyer F, Bekel T, Kaiser O, Pühler A, Niehaus K., J Biotechnol 106(2-3), 2003
PMID: 14651861
Comparison of the genomes of two Xanthomonas pathogens with differing host specificities.
da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El-Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA, Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos Santos M, Truffi D, Tsai SM, White FF, Setubal JC, Kitajima JP., Nature 417(6887), 2002
PMID: 12024217

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 11589581
PubMed | Europe PMC

Suchen in

Google Scholar