The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains

Tauch A, Götker S, Pühler A, Kalinowski J, Thierbach G (2002)
JOURNAL OF BIOTECHNOLOGY 99(1): 79-91.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The potential of the alanine racemase gene alr from Corynebacterium glutamicum ATCC 13032 to substitute for antibiotic resistance determinants in cloning systems has been investigated. The alr gene was identified by a PCR technique and its nucleotide sequence was determined. The deduced protein revealed the highest amino acid sequence similarity to the Alr protein from Mycobacterium smegmatis with 45% identical and 58% similar amino acids. A defined alr deletion mutant of C. glutamicum displayed a strict dependence on the presence of D-alanine for growth on complex and minimal medium. The alr gene was placed on a novel C. glutamicum vector which is completely free of antibiotic resistance genes. In vivo complementation of the chromosomal alr deletion with alr-carrying vectors permitted growth of the mutant strain in the absence of external D-alanine and provided strong selective pressure to maintain the plasmid. The alr gene enabled the selection of C. glutamicum transformants with a similar efficiency as the tetracycline resistance gene tetA (33). These data provided experimental evidence that the alr gene can be applied as an alternative selection marker to antibiotic resistance genes in industrial C. glutamicum strains. In an application example, the novel Deltaalr host-alr(+) vector-system for C. glutamicum was used to overproduce the vitamin D-pantothenic acid. (C) 2002 Elsevier Science B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
99
Ausgabe
1
Seite(n)
79-91
ISSN
PUB-ID

Zitieren

Tauch A, Götker S, Pühler A, Kalinowski J, Thierbach G. The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains. JOURNAL OF BIOTECHNOLOGY. 2002;99(1):79-91.
Tauch, A., Götker, S., Pühler, A., Kalinowski, J., & Thierbach, G. (2002). The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains. JOURNAL OF BIOTECHNOLOGY, 99(1), 79-91. doi:10.1016/S0168-1656(02)00159-1
Tauch, A., Götker, S., Pühler, A., Kalinowski, J., and Thierbach, G. (2002). The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains. JOURNAL OF BIOTECHNOLOGY 99, 79-91.
Tauch, A., et al., 2002. The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains. JOURNAL OF BIOTECHNOLOGY, 99(1), p 79-91.
A. Tauch, et al., “The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains”, JOURNAL OF BIOTECHNOLOGY, vol. 99, 2002, pp. 79-91.
Tauch, A., Götker, S., Pühler, A., Kalinowski, J., Thierbach, G.: The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains. JOURNAL OF BIOTECHNOLOGY. 99, 79-91 (2002).
Tauch, Andreas, Götker, S, Pühler, Alfred, Kalinowski, Jörn, and Thierbach, G. “The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains”. JOURNAL OF BIOTECHNOLOGY 99.1 (2002): 79-91.

26 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

A D-Alanine auxotrophic live vaccine is effective against lethal infection caused by Staphylococcus aureus.
Moscoso M, García P, Cabral MP, Rumbo C, Bou G., Virulence 9(1), 2018
PMID: 29297750
Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications.
Whitford CM, Dymek S, Kerkhoff D, März C, Schmidt O, Edich M, Droste J, Pucker B, Rückert C, Kalinowski J., J Biol Eng 12(), 2018
PMID: 30123321
Environmental roles of microbial amino acid racemases.
Hernández SB, Cava F., Environ Microbiol 18(6), 2016
PMID: 26419727
A method for simultaneous gene overexpression and inactivation in the Corynebacterium glutamicum genome.
Xu J, Zhang J, Han M, Zhang W., J Ind Microbiol Biotechnol 43(10), 2016
PMID: 27377799
Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes.
Vogt M, Haas S, Polen T, van Ooyen J, Bott M., Microb Biotechnol 8(2), 2015
PMID: 25488800
Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction.
Vogt M, Haas S, Klaffl S, Polen T, Eggeling L, van Ooyen J, Bott M., Metab Eng 22(), 2014
PMID: 24333966
Construction of a novel expression system for use in Corynebacterium glutamicum.
Hu J, Li Y, Zhang H, Tan Y, Wang X., Plasmid 75(), 2014
PMID: 25108235
New technologies in developing recombinant attenuated Salmonella vaccine vectors.
Wang S, Kong Q, Curtiss R., Microb Pathog 58(), 2013
PMID: 23142647
The Asd(+)-DadB(+) dual-plasmid system offers a novel means to deliver multiple protective antigens by a recombinant attenuated Salmonella vaccine.
Xin W, Wanda SY, Zhang X, Santander J, Scarpellini G, Ellis K, Alamuri P, Curtiss R., Infect Immun 80(10), 2012
PMID: 22868499
Upregulation of MetC is essential for D-alanine-independent growth of an alr/dadX-deficient Escherichia coli strain.
Kang L, Shaw AC, Xu D, Xia W, Zhang J, Deng J, Wöldike HF, Liu Y, Su J., J Bacteriol 193(5), 2011
PMID: 21193606
Corynebacterium glutamicum as a host for synthesis and export of D-Amino Acids.
Stäbler N, Oikawa T, Bott M, Eggeling L., J Bacteriol 193(7), 2011
PMID: 21257776
Tools for genetic manipulations in Corynebacterium glutamicum and their applications.
Nešvera J, Pátek M., Appl Microbiol Biotechnol 90(5), 2011
PMID: 21519933
Alanine racemase mutants of Burkholderia pseudomallei and Burkholderia mallei and use of alanine racemase as a non-antibiotic-based selectable marker.
Zajdowicz SL, Jones-Carson J, Vazquez-Torres A, Jobling MG, Gill RE, Holmes RK., PLoS One 6(6), 2011
PMID: 21720554
Gene cloning and characterization of a second alanine racemase from Bacillus subtilis encoded by yncD.
Pierce KJ, Salifu SP, Tangney M., FEMS Microbiol Lett 283(1), 2008
PMID: 18399999
Live bacterial vaccines--a review and identification of potential hazards.
Detmer A, Glenting J., Microb Cell Fact 5(), 2006
PMID: 16796731
Gene expression systems in corynebacteria.
Srivastava P, Deb JK., Protein Expr Purif 40(2), 2005
PMID: 15766862
Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling.
Hüser AT, Chassagnole C, Lindley ND, Merkamm M, Guyonvarch A, Elisáková V, Pátek M, Kalinowski J, Brune I, Pühler A, Tauch A., Appl Environ Microbiol 71(6), 2005
PMID: 15933028
Manipulating corynebacteria, from individual genes to chromosomes.
Vertès AA, Inui M, Yukawa H., Appl Environ Microbiol 71(12), 2005
PMID: 16332735
Selection and characterization of conditionally active promoters in Lactobacillus plantarum, using alanine racemase as a promoter probe.
Bron PA, Hoffer SM, Van Swam II, De Vos WM, Kleerebezem M., Appl Environ Microbiol 70(1), 2004
PMID: 14711657

39 References

Daten bereitgestellt von Europe PubMed Central.

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome.
Bathe B, Kalinowski J, Puhler A., Mol. Gen. Genet. 252(3), 1996
PMID: 8842145
Overexpression of the D-alanine racemase gene confers resistance to D-cycloserine in Mycobacterium smegmatis.
Caceres NE, Harris NB, Wellehan JF, Feng Z, Kapur V, Barletta RG., J. Bacteriol. 179(16), 1997
PMID: 9260945
Isolation of an alanine racemase gene from Bacillus subtilis and its use for plasmid maintenance in B. subtilis
Ferrari, Bio/Technology 3(), 1985
ASD-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other gram-negative bacteria.
Handfield M, Schweizer HP, Mahan MJ, Sanschagrin F, Hoang T, Levesque RC., BioTechniques 24(2), 1998
PMID: 9494727
Recent topics in pyridoxal 5'-phosphate enzyme studies.
Hayashi H, Wada H, Yoshimura T, Esaki N, Soda K., Annu. Rev. Biochem. 59(), 1990
PMID: 2197992
LION and Degussa apply genomics to fermentation.
Hodgson J., Nat. Biotechnol. 16(8), 1998
PMID: 9702763
The alanine racemase gene is essential for growth of Lactobacillus plantarum.
Hols P, Defrenne C, Ferain T, Derzelle S, Delplace B, Delcour J., J. Bacteriol. 179(11), 1997
PMID: 9171436
Protoplast transformation of glutamate-producing bacteria with plasmid DNA.
Katsumata R, Ozaki A, Oka T, Furuya A., J. Bacteriol. 159(1), 1984
PMID: 6145700
The fermentative production of L-lysine as an animal feed additive.
Kircher M, Pfefferle W., Chemosphere 43(1), 2001
PMID: 11233822
Amino acids-technical production and use
Leuchtenberger, 1996
High efficiency electroporation of intact C. glutamicum cells
Liebl, FEMS Microbiol. Lett. 65(), 1989
Selection of bacterial virulence genes that are specifically induced in host tissues.
Mahan MJ, Slauch JM, Mekalanos JJ., Science 259(5095), 1993
PMID: 8430319
Codon preference in corynebacteria.
Malumbres M, Gil JA, Martin JF., Gene 134(1), 1993
PMID: 8244028
Optimal alignments in linear space.
Myers EW, Miller W., Comput. Appl. Biosci. 4(1), 1988
PMID: 3382986
Plasmid pGA1 from Corynebacterium glutamicum codes for a gene product that positively influences plasmid copy number.
Nesvera J, Patek M, Hochmannova J, Abrhamova Z, Becvarova V, Jelinkova M, Vohradsky J., J. Bacteriol. 179(5), 1997
PMID: 9045809
Antibiotic resistance spread in food.
Perreten V, Schwarz F, Cresta L, Boeglin M, Dasen G, Teuber M., Nature 389(6653), 1997
PMID: 9349809

Rehm, 1993
Ammonia assimilation and the biosynthesis of glutamine, glutamate, aspartate, asparagine, l-alanine, and d-alanine
Reitzer, 1996

Sambrook, 1989
Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6.
Sonnen H, Thierbach G, Kautz S, Kalinowski J, Schneider J, Puhler A, Kutzner HJ., Gene 107(1), 1991
PMID: 1660431
A food-grade cloning system for industrial strains of Lactococcus lactis.
Sorensen KI, Larsen R, Kibenich A, Junge MP, Johansen E., Appl. Environ. Microbiol. 66(4), 2000
PMID: 10742196
The Staden sequence analysis package.
Staden R., Mol. Biotechnol. 5(3), 1996
PMID: 8837029
Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1.
Strych U, Huang HC, Krause KL, Benedik MJ., Curr. Microbiol. 41(4), 2000
PMID: 10977898
Characterization of the alanine racemases from two mycobacteria.
Strych U, Penland RL, Jimenez M, Krause KL, Benedik MJ., FEMS Microbiol. Lett. 196(2), 2001
PMID: 11267762
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915

AUTHOR UNKNOWN, 0
Pathogenicity and immunogenicity of a Listeria monocytogenes strain that requires D-alanine for growth.
Thompson RJ, Bouwer HG, Portnoy DA, Frankel FR., Infect. Immun. 66(8), 1998
PMID: 9673233
Containment of antibiotic resistance.
Williams RJ, Heymann DL., Science 279(5354), 1998
PMID: 9508688
Medical consequences of antibiotic use in agriculture.
Witte W., Science 279(5353), 1998
PMID: 9490487

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 12204559
PubMed | Europe PMC

Suchen in

Google Scholar