Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare

Finkemeier I, Kluge C, Metwally A, Georgi M, Grotjohann N, Dietz K-J (2003)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Finkemeier, I; Kluge, C; Metwally, A; Georgi, M; Grotjohann, Norbert; Dietz, Karl-JosefUniBi
Abstract / Bemerkung
The inter-relation between nitrogen availability and cadmium toxicity was studied in roots of barley seedlings with emphasis on the analysis of expression of 10 selected genes relevant for growth in the presence of toxic Cd concentrations. The response to Cd exposure differed quantitatively or qualitatively for the 10 genes in dependence of the N supply. Transcripts of glutathione synthase, glutathione reductase, glutathione peroxidase and dehydroascorbate reductase were measured as parameters involved in antioxidant defence, metallothionein, phosphoenolpyruvate carboxylase and phytochelatin synthase (PCS) were analysed as genes related to heavy metal binding, and vacuolar ATPase subunits VHA-E and VHA-c and a NRAMP-transporter as genes being implicated in Cd transport. Reprogramming of the Cd response was most obvious for PCS and NRAMP whose transcript levels were unaltered and down-regulated, respectively, in the presence of Cd at adequate N, but strongly up-regulated upon Cd exposure under conditions of nitrogen deficiency. Different responses to Cd at varying N supply were also seen for the antioxidant genes. The results on gene expression are discussed in context with the changes in biochemical parameters, and underline the importance of evaluating the general growth conditions of a plant when discussing its specific response to a stressor such as Cd. The sequence of the nramp cDNA was filed at the EMBL/GenBank/DDBJ Databases under the accession number AJ514946.
Hordeum vulgare; metallothionein; NRAMP; deficiency; phytochelatin synthase; transcript regulation; V-ATPase; nitrogen; cadmium; glutathione; root
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Finkemeier I, Kluge C, Metwally A, Georgi M, Grotjohann N, Dietz K-J. Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare. PLANT CELL AND ENVIRONMENT. 2003;26(6):821-833.
Finkemeier, I., Kluge, C., Metwally, A., Georgi, M., Grotjohann, N., & Dietz, K. - J. (2003). Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare. PLANT CELL AND ENVIRONMENT, 26(6), 821-833.
Finkemeier, I., Kluge, C., Metwally, A., Georgi, M., Grotjohann, N., and Dietz, K. - J. (2003). Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare. PLANT CELL AND ENVIRONMENT 26, 821-833.
Finkemeier, I., et al., 2003. Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare. PLANT CELL AND ENVIRONMENT, 26(6), p 821-833.
I. Finkemeier, et al., “Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare”, PLANT CELL AND ENVIRONMENT, vol. 26, 2003, pp. 821-833.
Finkemeier, I., Kluge, C., Metwally, A., Georgi, M., Grotjohann, N., Dietz, K.-J.: Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare. PLANT CELL AND ENVIRONMENT. 26, 821-833 (2003).
Finkemeier, I, Kluge, C, Metwally, A, Georgi, M, Grotjohann, Norbert, and Dietz, Karl-Josef. “Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare”. PLANT CELL AND ENVIRONMENT 26.6 (2003): 821-833.

24 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump.
Wang T, Hua Y, Chen M, Zhang J, Guan C, Zhang Z., Front Plant Sci 9(), 2018
PMID: 30619437
Vacuolar compartmentalization as indispensable component of heavy metal detoxification in plants.
Sharma SS, Dietz KJ, Mimura T., Plant Cell Environ 39(5), 2016
PMID: 26729300
Variation of antioxidants and secondary metabolites in nitrogen-deficient barley plants.
Kováčik J, Klejdus B, Babula P, Jarošová M., J Plant Physiol 171(3-4), 2014
PMID: 24054753
Plant peptides in defense and signaling.
Marmiroli N, Maestri E., Peptides 56(), 2014
PMID: 24681437
Soil cadmium enrichment: Allocation and plant physiological manifestations.
Irfan M, Hayat S, Ahmad A, Alyemeni MN., Saudi J Biol Sci 20(1), 2013
PMID: 23961213
Cadmium inhibits the induction of high-affinity nitrate uptake in maize (Zea mays L.) roots.
Rizzardo C, Tomasi N, Monte R, Varanini Z, Nocito FF, Cesco S, Pinton R., Planta 236(6), 2012
PMID: 22983671
Defense responses of soybean roots during exposure to cadmium, excess of nitrogen supply and combinations of these stressors.
Konotop Y, Mészáros P, Spieß N, Mistríková V, Piršelová B, Libantová J, Moravčíková J, Taran N, Hauptvogel P, Matušíková I., Mol Biol Rep 39(12), 2012
PMID: 22941249
Influence of long-term nitrogen fertilization on micronutrient density in grain of winter wheat (Triticum aestivum L.)
Shi Rongli, Zhang Yueqiang, Chen Xinping, Sun Qinping, Zhang Fusuo, Römheld Volker, Zou Chunqin., J Cereal Sci 51(1), 2010
PMID: IND44322233
The relationship between metal toxicity and cellular redox imbalance.
Sharma SS, Dietz KJ., Trends Plant Sci 14(1), 2009
PMID: 19070530
Quantitative detection of changes in the leaf-mesophyll tonoplast proteome in dependency of a cadmium exposure of barley (Hordeum vulgare L.) plants.
Schneider T, Schellenberg M, Meyer S, Keller F, Gehrig P, Riedel K, Lee Y, Eberl L, Martinoia E., Proteomics 9(10), 2009
PMID: 19391183
Interaction of heavy metals with the sulphur metabolism in angiosperms from an ecological point of view.
Ernst WH, Krauss GJ, Verkleij JA, Wesenberg D., Plant Cell Environ 31(1), 2008
PMID: 17999660
Plant peptides and peptidomics.
Farrokhi N, Whitelegge JP, Brusslan JA., Plant Biotechnol J 6(2), 2008
PMID: 18069950
Alterations of the gene expression, lipid peroxidation, proline and thiol content along the barley root exposed to cadmium.
Tamás L, Dudíková J, Durceková K, Halusková L, Huttová J, Mistrík I, Ollé M., J Plant Physiol 165(11), 2008
PMID: 18155806
Stress response in two strains of the aquatic hyphomycete Heliscus lugdunensis after exposure to cadmium and copper ions.
Braha B, Tintemann H, Krauss G, Ehrman J, Bärlocher F, Krauss GJ., Biometals 20(1), 2007
PMID: 16900400
Differential expression and regulation of antioxidative enzymes by cadmium in pea plants.
Romero-Puertas MC, Corpas FJ, Rodríguez-Serrano M, Gómez M, Del Río LA, Sandalio LM., J Plant Physiol 164(10), 2007
PMID: 17074418
Rapid alteration of cellular redox homeostasis upon exposure to cadmium and mercury in alfalfa seedlings.
Ortega-Villasante C, Hernández LE, Rellán-Alvarez R, Del Campo FF, Carpena-Ruiz RO., New Phytol 176(1), 2007
PMID: 17803643
Aluminium-induced drought and oxidative stress in barley roots.
Tamás L, Huttová J, Mistrík I, Simonovicová M, Siroká B., J Plant Physiol 163(7), 2006
PMID: 16616589
The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses.
Sarry JE, Kuhn L, Ducruix C, Lafaye A, Junot C, Hugouvieux V, Jourdain A, Bastien O, Fievet JB, Vailhen D, Amekraz B, Moulin C, Ezan E, Garin J, Bourguignon J., Proteomics 6(7), 2006
PMID: 16502469
Identification of mouse SLC39A8 as the transporter responsible for cadmium-induced toxicity in the testis.
Dalton TP, He L, Wang B, Miller ML, Jin L, Stringer KF, Chang X, Baxter CS, Nebert DW., Proc Natl Acad Sci U S A 102(9), 2005
PMID: 15722412
Molecular changes in Pisum sativum L. roots during arbuscular mycorrhiza buffering of cadmium stress.
Rivera-Becerril F, van Tuinen D, Martin-Laurent F, Metwally A, Dietz KJ, Gianinazzi S, Gianinazzi-Pearson V., Mycorrhiza 16(1), 2005
PMID: 16136340
Cadmium toxicity to barley (Hordeum vulgare) as affected by varying Fe nutritional status.
Sharma SS, Kaul S, Metwally A, Goyal KC, Finkemeier I, Dietz KJ., Plant Sci 166(5), 2004
PMID: IND43633715

45 References

Daten bereitgestellt von Europe PubMed Central.

Immunological characterization of two dominant tonoplast polypeptides.
Betz M, Dietz KJ., Plant Physiol. 97(4), 1991
PMID: 16668546

Brune, Journal of Plant Nutrition 18(), 1995
A gene family encoding glutathione peroxidase homologues in Hordeum vulgare (barley).
Churin Y, Schilling S, Borner T., FEBS Lett. 459(1), 1999
PMID: 10508912
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast.
Clemens S, Kim EJ, Neumann D, Schroeder JI., EMBO J. 18(12), 1999
PMID: 10369673
Phytochelatins and their roles in heavy metal detoxification.
Cobbett CS., Plant Physiol. 123(3), 2000
PMID: 10889232
Involvement of NRAMP1 from Arabidopsis thaliana in iron transport.
Curie C, Alonso JM, Le Jean M, Ecker JR, Briat JF., Biochem. J. 347 Pt 3(), 2000
PMID: 10769179

Dietz, 1996

Dietz, 1999
Subunit E of the vacuolar H(+)-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis.
Dietz KJ, Rudloff S, Ageorges A, Eckerskorn C, Fischer K, Arbinger B., Plant J. 8(4), 1995
PMID: 7496398
Significance of the V-type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level.
Dietz KJ, Tavakoli N, Kluge C, Mimura T, Sharma SS, Harris GC, Chardonnens AN, Golldack D., J. Exp. Bot. 52(363), 2001
PMID: 11559732

Faltin, Physiologia Plantarum 104(), 1998

The ZIP family of metal transporters.
Guerinot ML., Biochim. Biophys. Acta 1465(1-2), 2000
PMID: 10748254
Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe.
Ha SB, Smith AP, Howden R, Dietrich WM, Bugg S, O'Connell MJ, Goldsbrough PB, Cobbett CS., Plant Cell 11(6), 1999
PMID: 10368185

Hagemeyer, 1999
Enhanced copper tolerance in Silene vulgaris (Moench) Garcke populations from copper mines is associated with increased transcript levels of a 2b-type metallothionein gene.
van Hoof NA, Hassinen VH, Hakvoort HW, Ballintijn KF, Schat H, Verkleij JA, Ernst WH, Karenlampi SO, Tervahauta AI., Plant Physiol. 126(4), 2001
PMID: 11500550
Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of beta-actin transcripts as quantitative reference.
Kreuzer KA, Lass U, Landt O, Nitsche A, Laser J, Ellerbrok H, Pauli G, Huhn D, Schmidt CA., Clin. Chem. 45(2), 1999
PMID: 9931059

Marschner, 1995

Phylogenetic relationships within cation transporter families of Arabidopsis.
Maser P, Thomine S, Schroeder JI, Ward JM, Hirschi K, Sze H, Talke IN, Amtmann A, Maathuis FJ, Sanders D, Harper JF, Tchieu J, Gribskov M, Persans MW, Salt DE, Kim SA, Guerinot ML., Plant Physiol. 126(4), 2001
PMID: 11500563

An iron deficiency-specific cDNA from barley roots having two homologous cysteine-rich MT domains.
Okumura N, Nishizawa NK, Umehara Y, Mori S., Plant Mol. Biol. 17(3), 1991
PMID: 1832055

Prasad, 1999

Raven, New Phytologist 101(), 1985

Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots.
Schutzendubel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A., Plant Physiol. 127(3), 2001
PMID: 11706171

Cadmium and iron transport by members of a plant metal transporter family in Arabidopsis with homology to Nramp genes.
Thomine S, Wang R, Ward JM, Crawford NM, Schroeder JI., Proc. Natl. Acad. Sci. U.S.A. 97(9), 2000
PMID: 10781110
AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution.
Vatamaniuk OK, Mari S, Lu YP, Rea PA., Proc. Natl. Acad. Sci. U.S.A. 96(12), 1999
PMID: 10359847

Woolhouse, 1983


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