A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411

Hansmeier N, Chao T-C, Daschkey S, Muesken M, Kalinowski J, Pühler A, Tauch A (2007)
PROTEOMICS 7(7): 1076-1096.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Hansmeier, Nicole; Chao, Tzu-Chiao; Daschkey, Svenja; Muesken, Mathias; Kalinowski, JörnUniBi; Pühler, AlfredUniBi ; Tauch, AndreasUniBi
Abstract / Bemerkung
Corynebacterium jeikeium is a lipid-requiring pathogen that is considered as part of the normal microflora of the human skin and associated with severe nosocomial infections. Systematic reference maps of the cytoplasmic, cell surface-associated, and extracellular proteome fractions of the clinical isolate C. jeikeium K411 were examined by 2-DE coupled with MALDI-TOF MS. A sum total of 555 protein spots were identified by PMF, corresponding to 358 different proteins that were classified into functional categories and integrated into metabolic pathways. The majority of the proteins were linked to housekeeping functions in energy production and translation and to physiological processes in amino acid, carbohydrate, nucleotide, and lipid metabolism. A complete enzymatic machinery necessary to utilize exogenous fatty acids by beta-oxidation was detected in the cytoplasmic proteome fraction. In addition, several predicted virulence factors of C. jeikeium K411 were identified in the cell surface-associated and extracellular subproteome, including the cell surface proteins SurA and SurB, the surface-anchored pilus subunits SapA and SapB, the surface-anchored collagen adhesin CbpA, the cholesterol esterase Che, and the acid phosphatase AcpA.
extracellular proteins; proteome map; beta-oxidation; Corynebacterium jeikeium; virulence factors
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Hansmeier N, Chao T-C, Daschkey S, et al. A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411. PROTEOMICS. 2007;7(7):1076-1096.
Hansmeier, N., Chao, T. - C., Daschkey, S., Muesken, M., Kalinowski, J., Pühler, A., & Tauch, A. (2007). A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411. PROTEOMICS, 7(7), 1076-1096. https://doi.org/10.1002/pmic.200600833
Hansmeier, N., Chao, T. - C., Daschkey, S., Muesken, M., Kalinowski, J., Pühler, A., and Tauch, A. (2007). A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411. PROTEOMICS 7, 1076-1096.
Hansmeier, N., et al., 2007. A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411. PROTEOMICS, 7(7), p 1076-1096.
N. Hansmeier, et al., “A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411”, PROTEOMICS, vol. 7, 2007, pp. 1076-1096.
Hansmeier, N., Chao, T.-C., Daschkey, S., Muesken, M., Kalinowski, J., Pühler, A., Tauch, A.: A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411. PROTEOMICS. 7, 1076-1096 (2007).
Hansmeier, Nicole, Chao, Tzu-Chiao, Daschkey, Svenja, Muesken, Mathias, Kalinowski, Jörn, Pühler, Alfred, and Tauch, Andreas. “A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411”. PROTEOMICS 7.7 (2007): 1076-1096.

12 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

More than a Toxin: Protein Inventory of Clostridium tetani Toxoid Vaccines.
Möller J, Kraner ME, Burkovski A., Proteomes 7(2), 2019
PMID: 30988272
Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans.
Bittel M, Gastiger S, Amin B, Hofmann J, Burkovski A., Proteomes 6(2), 2018
PMID: 29673200
Corynebacterium jeikeium jk0268 constitutes for the 40 amino acid long PorACj, which forms a homooligomeric and anion-selective cell wall channel.
Abdali N, Barth E, Norouzy A, Schulz R, Nau WM, Kleinekathöfer U, Tauch A, Benz R., PLoS One 8(10), 2013
PMID: 24116064
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.
Pacheco LG, Slade SE, Seyffert N, Santos AR, Castro TL, Silva WM, Santos AV, Santos SG, Farias LM, Carvalho MA, Pimenta AM, Meyer R, Silva A, Scrivens JH, Oliveira SC, Miyoshi A, Dowson CG, Azevedo V., BMC Microbiol 11(1), 2011
PMID: 21241507
Proteomics of corynebacteria: From biotechnology workhorses to pathogens.
Poetsch A, Haussmann U, Burkovski A., Proteomics 11(15), 2011
PMID: 21674800
Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose.
Sivagnanam K, Raghavan VG, Shah M, Hettich RL, Verberkmoes NC, Lefsrud MG., Proteome Sci 9(), 2011
PMID: 22008648
A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture.
Janssen H, Döring C, Ehrenreich A, Voigt B, Hecker M, Bahl H, Fischer RJ., Appl Microbiol Biotechnol 87(6), 2010
PMID: 20617312
Enhanced structural and functional genome elucidation of the arsenite-oxidizing strain Herminiimonas arsenicoxydans by proteomics data.
Weiss S, Carapito C, Cleiss J, Koechler S, Turlin E, Coppee JY, Heymann M, Kugler V, Stauffert M, Cruveiller S, Médigue C, Van Dorsselaer A, Bertin PN, Arsène-Ploetze F., Biochimie 91(2), 2009
PMID: 18852016
A comprehensive proteome map of the Haemophilus parasuis serovar 5.
Zhou M, Zhang A, Guo Y, Liao Y, Chen H, Jin M., Proteomics 9(10), 2009
PMID: 19405026

56 References

Daten bereitgestellt von Europe PubMed Central.

Serious infections caused by diphtheroids.
Johnson WD, Kaye D., Ann. N. Y. Acad. Sci. 174(2), 1970
PMID: 5278136

Jackman, Syst. Appl. Microbiol. 9(), 1987
Corynebacterium group JK in a hematological ward: infections, colonization and environmental contamination.
Telander B, Lerner R, Palmblad J, Ringertz O., Scand. J. Infect. Dis. 20(1), 1988
PMID: 3163173
Coryneform bacteria in infectious diseases: clinical and laboratory aspects.
Coyle MB, Lipsky BA., Clin. Microbiol. Rev. 3(3), 1990
PMID: 2116939
Opportunistic coryneform organisms--residents of human skin.
Kazmierczak AK, Szarapinska-Kwaszewska JK, Szewczyk EM., Pol. J. Microbiol. 54(1), 2005
PMID: 16209092
Clinical microbiology of coryneform bacteria.
Funke G, von Graevenitz A, Clarridge JE 3rd, Bernard KA., Clin. Microbiol. Rev. 10(1), 1997
PMID: 8993861
Corynebacterium jeikeium endocarditis: a systematic overview spanning four decades.
Mookadam F, Cikes M, Baddour LM, Tleyjeh IM, Mookadam M., Eur. J. Clin. Microbiol. Infect. Dis. 25(6), 2006
PMID: 16767481

Lagrou, Diagn. Microbial. Infect. Dis. 30(), 1998
Coryneform bacteria isolated from blood cultures and their antibiotic susceptibilities.
Balci I, Eksi F, Bayram A., J. Int. Med. Res. 30(4), 2002
PMID: 12235925
Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora.
Tauch A, Kaiser O, Hain T, Goesmann A, Weisshaar B, Albersmeier A, Bekel T, Bischoff N, Brune I, Chakraborty T, Kalinowski J, Meyer F, Rupp O, Schneiker S, Viehoever P, Puhler A., J. Bacteriol. 187(13), 2005
PMID: 15968079
Probability-based protein identification by searching sequence databases using mass spectrometry data.
Perkins DN, Pappin DJ, Creasy DM, Cottrell JS., Electrophoresis 20(18), 1999
PMID: 10612281

Link, 1998
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
Nielsen H, Engelbrecht J, Brunak S, von Heijne G., Protein Eng. 10(1), 1997
PMID: 9051728
Prediction of twin-arginine signal peptides.
Bendtsen JD, Nielsen H, Widdick D, Palmer T, Brunak S., BMC Bioinformatics 6(), 2005
PMID: 15992409
Non-classical protein secretion in bacteria.
Bendtsen JD, Kiemer L, Fausboll A, Brunak S., BMC Microbiol. 5(), 2005
PMID: 16212653
Prediction of lipoprotein signal peptides in Gram-negative bacteria.
Juncker AS, Willenbrock H, Von Heijne G, Brunak S, Nielsen H, Krogh A., Protein Sci. 12(8), 2003
PMID: 12876315
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A, Larsson B, von Heijne G, Sonnhammer EL., J. Mol. Biol. 305(3), 2001
PMID: 11152613
A genomic perspective on protein families.
Tatusov RL, Koonin EV, Lipman DJ., Science 278(5338), 1997
PMID: 9381173
Membrane proteins and proteomics: un amour impossible?
Santoni V, Molloy M, Rabilloud T., Electrophoresis 21(6), 2000
PMID: 10786880
Mapping the membrane proteome of Corynebacterium glutamicum.
Schluesener D, Fischer F, Kruip J, Rogner M, Poetsch A., Proteomics 5(5), 2005
PMID: 15717325
The Tat protein export pathway.
Berks BC, Sargent F, Palmer T., Mol. Microbiol. 35(2), 2000
PMID: 10652088
Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope.
Navarre WW, Schneewind O., Microbiol. Mol. Biol. Rev. 63(1), 1999
PMID: 10066836
Protein sorting to the cell wall envelope of Gram-positive bacteria.
Ton-That H, Marraffini LA, Schneewind O., Biochim. Biophys. Acta 1694(1-3), 2004
PMID: 15546671
A comprehensive two-dimensional map of cytosolic proteins of Bacillus subtilis.
Buttner K, Bernhardt J, Scharf C, Schmid R, Mader U, Eymann C, Antelmann H, Volker A, Volker U, Hecker M., Electrophoresis 22(14), 2001
PMID: 11565787
Towards a phosphoproteome map of Corynebacterium glutamicum.
Bendt AK, Burkovski A, Schaffer S, Bott M, Farwick M, Hermann T., Proteomics 3(8), 2003
PMID: 12923788
Highly phosphorylated bacterial proteins.
Rosen R, Becher D, Buttner K, Biran D, Hecker M, Ron EZ., Proteomics 4(10), 2004
PMID: 15378765
ProteoMod: A new tool to quantitate protein post-translational modifications.
Kumar Y, Khachane A, Belwal M, Das S, Somsundaram K, Tatu U., Proteomics 4(6), 2004
PMID: 15174136
Protein deamidation.
Robinson NE., Proc. Natl. Acad. Sci. U.S.A. 99(8), 2002
PMID: 11959979

Halligan, Nucleic Acids Res. 1(), 2004
A proteome reference map and proteomic analysis of Bifidobacterium longum NCC2705.
Yuan J, Zhu L, Liu X, Li T, Zhang Y, Ying T, Wang B, Wang J, Dong H, Feng E, Li Q, Wang J, Wang H, Wei K, Zhang X, Huang C, Huang P, Huang L, Zeng M, Wang H., Mol. Cell Proteomics 5(6), 2006
PMID: 16549425

Hartmann, J. Arch. Microbiol. 182(), 2004
Towards the proteome of Burkholderia cenocepacia H111: setting up a 2-DE reference map.
Riedel K, Carranza P, Gehrig P, Potthast F, Eberl L., Proteomics 6(1), 2006
PMID: 16294309
Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100.
Watt SA, Wilke A, Patschkowski T, Niehaus K., Proteomics 5(1), 2005
PMID: 15619296
Inter- and intraclonal diversity of the Pseudomonas aeruginosa proteome manifests within the secretome.
Wehmhoner D, Haussler S, Tummler B, Jansch L, Bredenbruch F, Wehland J, Steinmetz I., J. Bacteriol. 185(19), 2003
PMID: 13129952
The extracellular and cytoplasmic proteomes of the non-virulent Bacillus anthracis strain UM23C1-2.
Antelmann H, Williams RC, Miethke M, Wipat A, Albrecht D, Harwood CR, Hecker M., Proteomics 5(14), 2005
PMID: 16121336
SecA2 functions in the secretion of superoxide dismutase A and in the virulence of Mycobacterium tuberculosis.
Braunstein M, Espinosa BJ, Chan J, Belisle JT, Jacobs WR Jr., Mol. Microbiol. 48(2), 2003
PMID: 12675804

Cerdeno-Tarraga, J. Nucleic Acids Res. 31(), 2003
Pilus and nonpilus bacterial adhesins: assembly and function in cell recognition.
Hultgren SJ, Abraham S, Caparon M, Falk P, St Geme JW 3rd, Normark S., Cell 73(5), 1993
PMID: 8098994
The solute-binding component of a putative Mn(II) ABC transporter (MntA) is a novel Bacillus anthracis virulence determinant.
Gat O, Mendelson I, Chitlaru T, Ariel N, Altboum Z, Levy H, Weiss S, Grosfeld H, Cohen S, Shafferman A., Mol. Microbiol. 58(2), 2005
PMID: 16194238
Immune responses to intracellular bacteria.
Raupach B, Kaufmann SH., Curr. Opin. Immunol. 13(4), 2001
PMID: 11498297
Surface protein adhesins of Staphylococcus aureus.
Foster TJ, Hook M., Trends Microbiol. 6(12), 1998
PMID: 10036727
Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis.
Manco S, Hernon F, Yesilkaya H, Paton JC, Andrew PW, Kadioglu A., Infect. Immun. 74(7), 2006
PMID: 16790774
Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens.
Lindahl G, Stalhammar-Carlemalm M, Areschoug T., Clin. Microbiol. Rev. 18(1), 2005
PMID: 15653821
Mosaicism in the alpha-like protein genes of group B streptococci.
Lachenauer CS, Creti R, Michel JL, Madoff LC., Proc. Natl. Acad. Sci. U.S.A. 97(17), 2000
PMID: 10944228
Group B streptococci escape host immunity by deletion of tandem repeat elements of the alpha C protein.
Madoff LC, Michel JL, Gong EW, Kling DE, Kasper DL., Proc. Natl. Acad. Sci. U.S.A. 93(9), 1996
PMID: 8633028
Characterization and sequencing of a respiratory burst-inhibiting acid phosphatase from Francisella tularensis.
Reilly TJ, Baron GS, Nano FE, Kuhlenschmidt MS., J. Biol. Chem. 271(18), 1996
PMID: 8631917


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