The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes

Nakunst D, Larisch C, Hueser AT, Tauch A, Pühler A, Kalinowski J (2007)
JOURNAL OF BACTERIOLOGY 189(13): 4696-4707.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The gene for the extracytoplasmic function (ECF) sigma factor SigM was deleted from the chromosome of the gram-positive soil bacterium Corynebacterium glutamicum to elucidate the role of the SigM protein in the regulation of gene expression. Comparative DNA microarray hybridizations of the C. glutamicum wild type and sigM-deficient mutant C. glutamicum DNI revealed 23 genes with enhanced expression in the sigM-proficient strain, encoding functions in the assembly of iron-sulfur clusters (suf operon), thioredoxin reductase (trxB), thioredoxins (trxC, trxB1), chaperones (groES, groEL, clpB), and proteins involved in the heat shock response (hspR, dnaJ, grpE). Deletion of the sigM gene rendered the C. glutamicum cells more sensitive to heat, cold, and the presence of the thiol oxidant diamide. Transcription of the sigM gene increased under different stress conditions, including heat shock, cold shock, and disulfide stress caused by diamide treatment, suggesting a regulatory role for SigM under thiol-oxidative stress conditions. Stress-responsive promoters were determined upstream of the suf operon and of the trxB, trxC, and trxB1 genes. The deduced SigM consensus promoter is characterized by the - 35 hexamer gGGAAT and the - 10 hexamer YGTTGR. Transcription of the sigM gene is apparently controlled by the ECF sigma factor SigH, since a sigH mutant was unable to enhance the expression of sigM and the SigM regulon under thioloxidative stress conditions. A typical SigH-responsive promoter was mapped upstream of the sigM gene. The ECF sigma factor SigM is apparently part of a regulatory cascade, and its transcription is controlled by SigH under conditions of thiol-oxidative stress.
Erscheinungsjahr
2007
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
189
Ausgabe
13
Seite(n)
4696-4707
ISSN
0021-9193
Page URI
https://pub.uni-bielefeld.de/record/1593371

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Nakunst D, Larisch C, Hueser AT, Tauch A, Pühler A, Kalinowski J. The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY. 2007;189(13):4696-4707.
Nakunst, D., Larisch, C., Hueser, A. T., Tauch, A., Pühler, A., & Kalinowski, J. (2007). The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY, 189(13), 4696-4707. doi:10.1128/JB.00382-07
Nakunst, D., Larisch, C., Hueser, A. T., Tauch, A., Pühler, A., and Kalinowski, J. (2007). The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY 189, 4696-4707.
Nakunst, D., et al., 2007. The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY, 189(13), p 4696-4707.
D. Nakunst, et al., “The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes”, JOURNAL OF BACTERIOLOGY, vol. 189, 2007, pp. 4696-4707.
Nakunst, D., Larisch, C., Hueser, A.T., Tauch, A., Pühler, A., Kalinowski, J.: The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. JOURNAL OF BACTERIOLOGY. 189, 4696-4707 (2007).
Nakunst, Diana, Larisch, Christof, Hueser, Andrea T., Tauch, Andreas, Pühler, Alfred, and Kalinowski, Jörn. “The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes”. JOURNAL OF BACTERIOLOGY 189.13 (2007): 4696-4707.

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