Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum

Koopmeiners S, Gilzer D, Widmann C, Berelsmann N, Sproß J, Niemann H, Fischer von Mollard G (2024)
FEBS letters.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
l-Amino acid oxidases (LAAOs) catalyze the oxidative deamination of l-amino acids to alpha-keto acids. Recombinant production of LAAOs with broad substrate spectrum remains a formidable challenge. We previously achieved this for the highly active and thermostable LAAO4 of Hebeloma cylindrosporum (HcLAAO4). Here, we crystallized a proteolytically truncated surface entropy reduction variant of HcLAAO4 and solved its structure in substrate-free form and in complex with diverse substrates. The ability to support the aliphatic portion of a substrate's side chain by an overall hydrophobic active site is responsible for the broad substrate spectrum of HcLAAO4, including l-amino acids with big aromatic, acidic and basic side chains. Based on the structural findings, we generated an E288H variant with increased activity toward pharmaceutical building blocks of high interest. © 2024 The Author(s). FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
Erscheinungsjahr
2024
Zeitschriftentitel
FEBS letters
eISSN
1873-3468
Page URI
https://pub.uni-bielefeld.de/record/2992103

Zitieren

Koopmeiners S, Gilzer D, Widmann C, et al. Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum. FEBS letters. 2024.
Koopmeiners, S., Gilzer, D., Widmann, C., Berelsmann, N., Sproß, J., Niemann, H., & Fischer von Mollard, G. (2024). Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum. FEBS letters. https://doi.org/10.1002/1873-3468.15002
Koopmeiners, Simon, Gilzer, Dominic, Widmann, Christiane, Berelsmann, Nils, Sproß, Jens, Niemann, Hartmut, and Fischer von Mollard, Gabriele. 2024. “Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum”. FEBS letters.
Koopmeiners, S., Gilzer, D., Widmann, C., Berelsmann, N., Sproß, J., Niemann, H., and Fischer von Mollard, G. (2024). Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum. FEBS letters.
Koopmeiners, S., et al., 2024. Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum. FEBS letters.
S. Koopmeiners, et al., “Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum”, FEBS letters, 2024.
Koopmeiners, S., Gilzer, D., Widmann, C., Berelsmann, N., Sproß, J., Niemann, H., Fischer von Mollard, G.: Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum. FEBS letters. (2024).
Koopmeiners, Simon, Gilzer, Dominic, Widmann, Christiane, Berelsmann, Nils, Sproß, Jens, Niemann, Hartmut, and Fischer von Mollard, Gabriele. “Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum”. FEBS letters (2024).

Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

References

Daten bereitgestellt von Europe PubMed Central.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 39152524
PubMed | Europe PMC

Suchen in

Google Scholar