Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate

Sagurna, Leonie; Heinrich, Sascha; Kaufmann, Lara-Sophie; Rückert-Reed, Christian; Busche, Tobias; Wolf, Alexander; Eickhoff, Jan; Klebl, Bert; Kalinowski, Jörn; Bandow, Julia E.

Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate

Sagurna L, Heinrich S, Kaufmann L-S, Rückert-Reed C, Busche T, Wolf A, Eickhoff J, Klebl B, Kalinowski J, Bandow JE (2023)
Antibiotics 12(7): 1116.

Zeitschriftenaufsatz | Veröffentlicht | Englisch

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antibiotics-12-01116.pdf 2.10 MB

URL

https://www.mdpi.com/2079-6382/12/7/1116

DOI

https://doi.org/10.3390/antibiotics12071116

URN

urn:nbn:de:0070-pub-29803736

Autor*in

Sagurna, Leonie; Heinrich, Sascha; Kaufmann, Lara-Sophie; Rückert-Reed, Christian^UniBi ; Busche, Tobias^UniBi; Wolf, Alexander; Eickhoff, Jan; Klebl, Bert; Kalinowski, Jörn^UniBi; Bandow, Julia E.

Einrichtung

Centrum für Biotechnologie > Arbeitsgruppe J. Kalinowski
Centrum für Biotechnologie > Technologieplattformen > Technologieplattform Genomik

Abstract / Bemerkung

Bacteria of the genus Streptomyces produce various specialized metabolites. Single biosynthetic gene clusters (BGCs) can give rise to different products that can vary in terms of their biological activities. For example, for alnumycin and the shunt product K115, antimicrobial activity was described, while no antimicrobial activity was detected for the shunt product 1,6-dihydro 8-propylanthraquinone. To investigate the antibacterial activity of 1,6-dihydro 8-propylanthraquinone, we produced alnumycin and 1,6-dihydro 8-propylanthraquinone from a Streptomyces isolate containing the alnumycin BGC. The strain was cultivated in liquid glycerol–nitrate–casein medium (GN), and both compounds were isolated using an activity and mass spectrometry-guided purification. The structures were validated via nuclear magnetic resonance (NMR) spectroscopy. A minimal inhibitory concentration (MIC) test revealed that 1,6-dihydro 8-propylanthraquinone exhibits antimicrobial activity against E. coli ΔtolC, B. subtilis, an S. aureus type strain, and a vancomycin intermediate-resistance S. aureus strain (VISA). Activity of 1,6-dihydro 8-propylanthraquinone against E. coli ΔtolC was approximately 10-fold higher than that of alnumycin. We were unable to confirm gyrase inhibition for either compound and believe that the modes of action of both compounds are worth reinvestigating.

Erscheinungsjahr

2023

Zeitschriftentitel

Antibiotics

Band

Ausgabe

Art.-Nr.

1116

Urheberrecht / Lizenzen

Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0)

eISSN

2079-6382

Page URI

https://pub.uni-bielefeld.de/record/2980373

Zitieren

Sagurna L, Heinrich S, Kaufmann L-S, et al. Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate. Antibiotics. 2023;12(7): 1116.

Sagurna, L., Heinrich, S., Kaufmann, L. - S., Rückert-Reed, C., Busche, T., Wolf, A., Eickhoff, J., et al. (2023). Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate. Antibiotics, 12(7), 1116. https://doi.org/10.3390/antibiotics12071116

Sagurna, Leonie, Heinrich, Sascha, Kaufmann, Lara-Sophie, Rückert-Reed, Christian, Busche, Tobias, Wolf, Alexander, Eickhoff, Jan, Klebl, Bert, Kalinowski, Jörn, and Bandow, Julia E. 2023. “Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate”. Antibiotics 12 (7): 1116.

Sagurna, L., Heinrich, S., Kaufmann, L. - S., Rückert-Reed, C., Busche, T., Wolf, A., Eickhoff, J., Klebl, B., Kalinowski, J., and Bandow, J. E. (2023). Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate. Antibiotics 12:1116.

Sagurna, L., et al., 2023. Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate. Antibiotics, 12(7): 1116.

L. Sagurna, et al., “Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate”, Antibiotics, vol. 12, 2023, : 1116.

Sagurna, L., Heinrich, S., Kaufmann, L.-S., Rückert-Reed, C., Busche, T., Wolf, A., Eickhoff, J., Klebl, B., Kalinowski, J., Bandow, J.E.: Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate. Antibiotics. 12, : 1116 (2023).

Sagurna, Leonie, Heinrich, Sascha, Kaufmann, Lara-Sophie, Rückert-Reed, Christian, Busche, Tobias, Wolf, Alexander, Eickhoff, Jan, Klebl, Bert, Kalinowski, Jörn, and Bandow, Julia E. “Characterization of the Antibacterial Activity of Quinone-Based Compounds Originating from the Alnumycin Biosynthetic Gene Cluster of a Streptomyces Isolate”. Antibiotics 12.7 (2023): 1116.

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