The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110

Nölting S, März C, Jacob L, Persicke M, Schneiker-Bekel S, Kalinowski J (2023)
Microorganisms 11(4): 848.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The pseudo-tetrasaccharide acarbose, produced by Actinoplanes sp. SE50/110, is a alpha-glucosidase inhibitor used for treatment of type 2 diabetes patients. In industrial production of acarbose, by-products play a relevant role that complicates the purification of the product and reduce yields. Here, we report that the acarbose 4-alpha-glucanotransferase AcbQ modifies acarbose and the phosphorylated version acarbose 7-phosphate. Elongated acarviosyl metabolites (alpha-acarviosyl-(1,4)-maltooligosaccharides) with one to four additional glucose molecules were identified performing in vitro assays with acarbose or acarbose 7-phosphate and short alpha-1,4-glucans (maltose, maltotriose and maltotetraose). High functional similarities to the 4-alpha-glucanotransferase MalQ, which is essential in the maltodextrin pathway, are revealed. However, maltotriose is a preferred donor and acarbose and acarbose 7-phosphate, respectively, serve as specific acceptors for AcbQ. This study displays the specific intracellular assembly of longer acarviosyl metabolites catalyzed by AcbQ, indicating that AcbQ is directly involved in the formation of acarbose by-products of Actinoplanes sp. SE50/110.
Erscheinungsjahr
2023
Zeitschriftentitel
Microorganisms
Band
11
Ausgabe
4
Art.-Nr.
848
eISSN
2076-2607
Page URI
https://pub.uni-bielefeld.de/record/2978776

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Nölting S, März C, Jacob L, Persicke M, Schneiker-Bekel S, Kalinowski J. The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110. Microorganisms. 2023;11(4): 848.
Nölting, S., März, C., Jacob, L., Persicke, M., Schneiker-Bekel, S., & Kalinowski, J. (2023). The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110. Microorganisms, 11(4), 848. https://doi.org/10.3390/microorganisms11040848
Nölting, Sophia, März, Camilla, Jacob, Lucas, Persicke, Marcus, Schneiker-Bekel, Susanne, and Kalinowski, Jörn. 2023. “The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110”. Microorganisms 11 (4): 848.
Nölting, S., März, C., Jacob, L., Persicke, M., Schneiker-Bekel, S., and Kalinowski, J. (2023). The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110. Microorganisms 11:848.
Nölting, S., et al., 2023. The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110. Microorganisms, 11(4): 848.
S. Nölting, et al., “The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110”, Microorganisms, vol. 11, 2023, : 848.
Nölting, S., März, C., Jacob, L., Persicke, M., Schneiker-Bekel, S., Kalinowski, J.: The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110. Microorganisms. 11, : 848 (2023).
Nölting, Sophia, März, Camilla, Jacob, Lucas, Persicke, Marcus, Schneiker-Bekel, Susanne, and Kalinowski, Jörn. “The 4-alpha-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110”. Microorganisms 11.4 (2023): 848.
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2024-01-15T11:57:12Z
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