Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074

Tseduliak, Vasylyna-Marta; Dolia, Borys; Ostash, Iryna; Lopatniuk, Maria; Busche, Tobias; Ochi, Kozo; Kalinowski, Jörn; Luzhetskyy, Andriy; Fedorenko, Victor; Ostash, Bohdan

Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074

Tseduliak V-M, Dolia B, Ostash I, Lopatniuk M, Busche T, Ochi K, Kalinowski J, Luzhetskyy A, Fedorenko V, Ostash B (2022)
Journal of Applied Genetics.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch

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DOI

https://doi.org/10.1007/s13353-022-00738-4

Autor*in

Tseduliak, Vasylyna-Marta; Dolia, Borys; Ostash, Iryna; Lopatniuk, Maria; Busche, Tobias^UniBi; Ochi, Kozo; Kalinowski, Jörn^UniBi; Luzhetskyy, Andriy; Fedorenko, Victor; Ostash, Bohdan

Einrichtung

Centrum für Biotechnologie - CeBiTec > Omics Core Facility - Leitung Next-Generation Sequencing
Centrum für Biotechnologie > Technologieplattformen > Technologieplattform Genomik

Abstract / Bemerkung

Streptomyces albus J1074 is one of the most popular heterologous expression platforms among streptomycetes. Identification of new genes and mutations that influence specialized metabolism in this species is therefore of great applied interest. Here, we describe S. albus KO-1304 that was isolated as a spontaneous lincomycin-resistant variant of double rpsL(R94G)rsmG(R15SG40E) mutant KO-1295. Besides altered antibiotic resistance profile, KO-1304 exhibited increased antibiotic activity as compared to its parental strains. KO-1304 genome sequencing revealed mutations within gene XNR_2147 encoding putative TetR-like protein. Gene XNR_2146 for efflux protein is the most likely target of repressing action of Xnr_2147. Our data agree with the scenario where lincomycin resistance phenotype of KO-1304 arose from inability of mutated Xnr_2147 protein to repress XNR_2146. Introduction of additional copy of XNR_2146 into wild type strain increased antibiotic activity of the latter, attesting to the practical value of transporter genes for strain improvement.

Stichworte

Streptomyces albus J1074; XNR_2146; Antibiotics; Lincomycin; Antibiotic; resistance; Transcriptional repressor; Strain improvement

Erscheinungsjahr

2022

Zeitschriftentitel

Journal of Applied Genetics

ISSN

1234-1983

eISSN

2190-3883

Page URI

https://pub.uni-bielefeld.de/record/2967393

Zitieren

Tseduliak V-M, Dolia B, Ostash I, et al. Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074. Journal of Applied Genetics. 2022.

Tseduliak, V. - M., Dolia, B., Ostash, I., Lopatniuk, M., Busche, T., Ochi, K., Kalinowski, J., et al. (2022). Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074. Journal of Applied Genetics. https://doi.org/10.1007/s13353-022-00738-4

Tseduliak, Vasylyna-Marta, Dolia, Borys, Ostash, Iryna, Lopatniuk, Maria, Busche, Tobias, Ochi, Kozo, Kalinowski, Jörn, Luzhetskyy, Andriy, Fedorenko, Victor, and Ostash, Bohdan. 2022. “Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074”. Journal of Applied Genetics.

Tseduliak, V. - M., Dolia, B., Ostash, I., Lopatniuk, M., Busche, T., Ochi, K., Kalinowski, J., Luzhetskyy, A., Fedorenko, V., and Ostash, B. (2022). Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074. Journal of Applied Genetics.

Tseduliak, V.-M., et al., 2022. Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074. Journal of Applied Genetics.

V.-M. Tseduliak, et al., “Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074”, Journal of Applied Genetics, 2022.

Tseduliak, V.-M., Dolia, B., Ostash, I., Lopatniuk, M., Busche, T., Ochi, K., Kalinowski, J., Luzhetskyy, A., Fedorenko, V., Ostash, B.: Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074. Journal of Applied Genetics. (2022).

Tseduliak, Vasylyna-Marta, Dolia, Borys, Ostash, Iryna, Lopatniuk, Maria, Busche, Tobias, Ochi, Kozo, Kalinowski, Jörn, Luzhetskyy, Andriy, Fedorenko, Victor, and Ostash, Bohdan. “Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J1074”. Journal of Applied Genetics (2022).

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