PUB - Publikationen an der Universität Bielefeld

The phylloxera resistant rootstock cultivar ′Boerner′ is an interspecies hybrid derived from *Vitis riparia* and *V. cinerea* and a valuable resource for *Vitis* disease resistances. We created a fully phased, high-quality ′Boerner′ genome sequence named BoeRC using long PacBio reads. Comprehensive gene annotation of both ′Boerner′ haplotypes, designated BoeRip and BoeCin, was applied to describe the phylloxera resistance locus *Rdv1*. Using a mapping population derived from a sensitive *V. vinifera* cultivar and ′Boerner′, the *Rdv1* locus was further delimited. *Rdv1*, which is derived from *V. cinerea* and included in the haplotype BoeCin, was compared with sequences of phylloxera susceptible and phylloxera tolerant cultivars. Between flanking regions that display high synteny, we detected and precisely characterized a diverse sequence region that covers between 202 to 403 kbp in different haplotypes. In BoeCin, five putative disease resistance genes were identified that represent likely candidates for conferring resistance to phylloxera.