Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding

Herrmann L, Schelletter L, Hoffrogge R, Niehaus K, Rudolph V, Farr M (2022)
Molecular Biology Reports .

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Herrmann, Leonie; Schelletter, LouiseUniBi; Hoffrogge, RaimundUniBi ; Niehaus, KarstenUniBi; Rudolph, Volker; Farr, Martin
Abstract / Bemerkung
BACKGROUND: During viral-induced myocarditis, immune cells migrate towards the site of infection and secrete proteases, which in turn can act as sheddases by cleaving extracellular domains of transmembrane proteins. We were interested in the shedding of the Coxsackie- and adenovirus receptor (CAR) that acts as an entry receptor for both eponymous viruses, which cause myocarditis. CAR shedding by secreted immune proteases could result in a favourable outcome of myocarditis as CAR's extracellular domain would be removed from the cardiomyocytes' surface leading to decreased susceptibility to ongoing viral infections.; METHODS AND RESULTS: In this work, matrix metalloproteinases and serine proteinases were screened for their proteolytic activity towards human CAR. Whereas matrix metalloproteinases, proteinase 3, and cathepsin G did not cleave human recombinant CAR or only within long incubation times, neutrophil elastase showed a distinct cleavage pattern of CAR's extracellular domain that was time- and dose-dependent. Neutrophil elastase cleaves CAR at its membrane-proximal immunoglobulin domain as we determined by nanoLC-MS/MS. Furthermore, neutrophil elastase treatment of cells reduced CAR surface levels as seen by flow cytometry and immunofluorescence microscopy.; CONCLUSIONS: With this study, we show that CAR might be a target for shedding by neutrophil elastase. © 2022. The Author(s).
Erscheinungsjahr
2022
Zeitschriftentitel
Molecular Biology Reports
eISSN
1573-4978
Page URI
https://pub.uni-bielefeld.de/record/2961212

Zitieren

Herrmann L, Schelletter L, Hoffrogge R, Niehaus K, Rudolph V, Farr M. Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding. Molecular Biology Reports . 2022.
Herrmann, L., Schelletter, L., Hoffrogge, R., Niehaus, K., Rudolph, V., & Farr, M. (2022). Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding. Molecular Biology Reports . https://doi.org/10.1007/s11033-022-07153-2
Herrmann, Leonie, Schelletter, Louise, Hoffrogge, Raimund, Niehaus, Karsten, Rudolph, Volker, and Farr, Martin. 2022. “Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding”. Molecular Biology Reports .
Herrmann, L., Schelletter, L., Hoffrogge, R., Niehaus, K., Rudolph, V., and Farr, M. (2022). Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding. Molecular Biology Reports .
Herrmann, L., et al., 2022. Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding. Molecular Biology Reports .
L. Herrmann, et al., “Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding”, Molecular Biology Reports , 2022.
Herrmann, L., Schelletter, L., Hoffrogge, R., Niehaus, K., Rudolph, V., Farr, M.: Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding. Molecular Biology Reports . (2022).
Herrmann, Leonie, Schelletter, Louise, Hoffrogge, Raimund, Niehaus, Karsten, Rudolph, Volker, and Farr, Martin. “Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding”. Molecular Biology Reports (2022).
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 35122600
PubMed | Europe PMC

Suchen in

Google Scholar