SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo

Fulde M, van Vorst K, Zhang K, Westermann AJ, Busche T, Huei YC, Welitschanski K, Froh I, Pagelow D, Plendl J, Pfarrer C, et al. (2021)
Gut microbes 13(1): 1973836.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Fulde, Marcus; van Vorst, Kira; Zhang, Kaiyi; Westermann, Alexander J; Busche, TobiasUniBi; Huei, Yong Chiun; Welitschanski, Katharina; Froh, Isabell; Pagelow, Dennis; Plendl, Johanna; Pfarrer, Christiane; Kalinowski, JörnUniBi
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Abstract / Bemerkung
Salmonella pathogenicity island (SPI) 2 type three secretion system (T3SS)-mediated effector molecules facilitate bacterial survival in phagocytes but their role in the intestinal epithelium in vivo remains ill-defined. Using our neonatal murine infection model in combination with SPI2 reporter technology and RNA-Seq of sorted primary enterocytes, we demonstrate expression of SPI2 effector molecules by intraepithelial Salmonella Typhimurium (S. Typhimurium). Contrary to expectation, immunostaining revealed that infection with SPI2 T3SS-mutants resulted in significantly enlarged intraepithelial Salmonella-containing vacuoles (SCV) with altered cellular positioning, suggesting impaired apical to basolateral transmigration. Also, infection with isogenic tagged S. Typhimurium strains revealed a reduced spread of intraepithelial SPI2 T3SS mutant S. Typhimurium to systemic body sites. These results suggest that SPI2 T3SS effector molecules contribute to enterocyte apical to basolateral transmigration of the SCV during the early stage of the infection.
Erscheinungsjahr
2021
Zeitschriftentitel
Gut microbes
Band
13
Ausgabe
1
Art.-Nr.
1973836
eISSN
1949-0984
Page URI
https://pub.uni-bielefeld.de/record/2957898

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Fulde M, van Vorst K, Zhang K, et al. SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo. Gut microbes. 2021;13(1): 1973836.
Fulde, M., van Vorst, K., Zhang, K., Westermann, A. J., Busche, T., Huei, Y. C., Welitschanski, K., et al. (2021). SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo. Gut microbes, 13(1), 1973836. https://doi.org/10.1080/19490976.2021.1973836
Fulde, M., van Vorst, K., Zhang, K., Westermann, A. J., Busche, T., Huei, Y. C., Welitschanski, K., Froh, I., Pagelow, D., Plendl, J., et al. (2021). SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo. Gut microbes 13:1973836.
Fulde, M., et al., 2021. SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo. Gut microbes, 13(1): 1973836.
M. Fulde, et al., “SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo”, Gut microbes, vol. 13, 2021, : 1973836.
Fulde, M., van Vorst, K., Zhang, K., Westermann, A.J., Busche, T., Huei, Y.C., Welitschanski, K., Froh, I., Pagelow, D., Plendl, J., Pfarrer, C., Kalinowski, J., Vogel, J., Valentin-Weigand, P., Hensel, M., Tedin, K., Repnik, U., Hornef, M.W.: SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo. Gut microbes. 13, : 1973836 (2021).
Fulde, Marcus, van Vorst, Kira, Zhang, Kaiyi, Westermann, Alexander J, Busche, Tobias, Huei, Yong Chiun, Welitschanski, Katharina, Froh, Isabell, Pagelow, Dennis, Plendl, Johanna, Pfarrer, Christiane, Kalinowski, Jörn, Vogel, Jorg, Valentin-Weigand, Peter, Hensel, Michael, Tedin, Karsten, Repnik, Urska, and Hornef, Mathias W. “SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo”. Gut microbes 13.1 (2021): 1973836.

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