Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB

Geerds C, Haas A, Niemann H (2021)
Acta crystallographica. Section F: Structural biology communications 77(Pt 8): 246-253.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Virulence-associated proteins (Vaps) contribute to the virulence of the pathogen Rhodococcus equi, but their mode of action has remained elusive. All Vaps share a conserved core of about 105 amino acids that folds into a compact eight-stranded antiparallel beta-barrel with a unique topology. At the top of the barrel, four loops connect the eight beta-strands. Previous Vap structures did not show concave surfaces that might serve as a ligand-binding site. Here, the structure of VapB in a new crystal form was determined at 1.71 A resolution. The asymmetric unit contains two molecules. In one of them, the loop regions at the top of the barrel adopt a different conformation from other Vap structures. An outward movement of the loops results in the formation of a hydrophobic cavity that might act as a ligand-binding site. This lends further support to the hypothesis that the structural similarity between Vaps and avidins suggests a potential binding function for Vaps. open access.
Erscheinungsjahr
2021
Zeitschriftentitel
Acta crystallographica. Section F: Structural biology communications
Band
77
Ausgabe
Pt 8
Art.-Nr.
246-253
eISSN
2053-230X
Page URI
https://pub.uni-bielefeld.de/record/2956908

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Geerds C, Haas A, Niemann H. Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB. Acta crystallographica. Section F: Structural biology communications. 2021;77(Pt 8): 246-253.
Geerds, C., Haas, A., & Niemann, H. (2021). Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB. Acta crystallographica. Section F: Structural biology communications, 77(Pt 8), 246-253. https://doi.org/10.1107/S2053230X2100738X
Geerds, C., Haas, A., and Niemann, H. (2021). Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB. Acta crystallographica. Section F: Structural biology communications 77: 246-253.
Geerds, C., Haas, A., & Niemann, H., 2021. Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB. Acta crystallographica. Section F: Structural biology communications, 77(Pt 8): 246-253.
C. Geerds, A. Haas, and H. Niemann, “Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB”, Acta crystallographica. Section F: Structural biology communications, vol. 77, 2021, : 246-253.
Geerds, C., Haas, A., Niemann, H.: Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB. Acta crystallographica. Section F: Structural biology communications. 77, : 246-253 (2021).
Geerds, Christina, Haas, Albert, and Niemann, Hartmut. “Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB”. Acta crystallographica. Section F: Structural biology communications 77.Pt 8 (2021): 246-253.
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2021-09-24T15:17:29Z
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