LipidCreator workbench to probe the lipidomic landscape
Peng B, Kopczynski D, Pratt BS, Ejsing CS, Burla B, Hermansson M, Benke PI, Tan SH, Chan MY, Torta F, Schwudke D, et al. (2020)
Nature Communications 11(1): 2057.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Peng, Bing;
Kopczynski, Dominik;
Pratt, Brian S.;
Ejsing, Christer S.;
Burla, Bo;
Hermansson, Martin;
Benke, Peter Imre;
Tan, Sock Hwee;
Chan, Mark Y.;
Torta, Federico;
Schwudke, Dominik;
Meckelmann, Sven W.
Alle
Alle
Einrichtung
Abstract / Bemerkung
**Abstract**
Mass spectrometry (MS)-based targeted lipidomics enables the robust quantification of selected lipids under various biological conditions but comprehensive software tools to support such analyses are lacking. Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope labeling, optimize collision energy and generate in silico spectral libraries. We validate LipidCreator assays computationally and analytically and prove that it is capable to generate large targeted experiments to analyze blood and to dissect lipid-signaling pathways such as in human platelets.
Mass spectrometry (MS)-based targeted lipidomics enables the robust quantification of selected lipids under various biological conditions but comprehensive software tools to support such analyses are lacking. Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope labeling, optimize collision energy and generate in silico spectral libraries. We validate LipidCreator assays computationally and analytically and prove that it is capable to generate large targeted experiments to analyze blood and to dissect lipid-signaling pathways such as in human platelets.
Erscheinungsjahr
2020
Zeitschriftentitel
Nature Communications
Band
11
Ausgabe
1
Art.-Nr.
2057
Urheberrecht / Lizenzen
eISSN
2041-1723
Page URI
https://pub.uni-bielefeld.de/record/2956707
Zitieren
Peng B, Kopczynski D, Pratt BS, et al. LipidCreator workbench to probe the lipidomic landscape. Nature Communications. 2020;11(1): 2057.
Peng, B., Kopczynski, D., Pratt, B. S., Ejsing, C. S., Burla, B., Hermansson, M., Benke, P. I., et al. (2020). LipidCreator workbench to probe the lipidomic landscape. Nature Communications, 11(1), 2057. https://doi.org/10.1038/s41467-020-15960-z
Peng, Bing, Kopczynski, Dominik, Pratt, Brian S., Ejsing, Christer S., Burla, Bo, Hermansson, Martin, Benke, Peter Imre, et al. 2020. “LipidCreator workbench to probe the lipidomic landscape”. Nature Communications 11 (1): 2057.
Peng, B., Kopczynski, D., Pratt, B. S., Ejsing, C. S., Burla, B., Hermansson, M., Benke, P. I., Tan, S. H., Chan, M. Y., Torta, F., et al. (2020). LipidCreator workbench to probe the lipidomic landscape. Nature Communications 11:2057.
Peng, B., et al., 2020. LipidCreator workbench to probe the lipidomic landscape. Nature Communications, 11(1): 2057.
B. Peng, et al., “LipidCreator workbench to probe the lipidomic landscape”, Nature Communications, vol. 11, 2020, : 2057.
Peng, B., Kopczynski, D., Pratt, B.S., Ejsing, C.S., Burla, B., Hermansson, M., Benke, P.I., Tan, S.H., Chan, M.Y., Torta, F., Schwudke, D., Meckelmann, S.W., Coman, C., Schmitz, O.J., MacLean, B., Manke, M.-C., Borst, O., Wenk, M.R., Hoffmann, N., Ahrends, R.: LipidCreator workbench to probe the lipidomic landscape. Nature Communications. 11, : 2057 (2020).
Peng, Bing, Kopczynski, Dominik, Pratt, Brian S., Ejsing, Christer S., Burla, Bo, Hermansson, Martin, Benke, Peter Imre, Tan, Sock Hwee, Chan, Mark Y., Torta, Federico, Schwudke, Dominik, Meckelmann, Sven W., Coman, Cristina, Schmitz, Oliver J., MacLean, Brendan, Manke, Mailin-Christin, Borst, Oliver, Wenk, Markus R., Hoffmann, Nils, and Ahrends, Robert. “LipidCreator workbench to probe the lipidomic landscape”. Nature Communications 11.1 (2020): 2057.
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