The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation

Hess N, Richter S, Liebthal M, Dietz K-J, Mustroph A (2021)
Antioxidants 10(3): 401.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Hess, Natalia; Richter, Simon; Liebthal, MichaelUniBi; Dietz, Karl-JosefUniBi; Mustroph, Angelika
Abstract / Bemerkung
The chloroplast primary metabolism is of central importance for plant growth and performance. Therefore, it is tightly regulated in order to adequately respond to multiple environmental conditions. A major fluctuation that plants experience each day is the change between day and night, i.e., the change between assimilation and dissimilation. Among other mechanisms, thioredoxin-mediated redox regulation is an important component of the regulation of plastid-localized metabolic enzymes. While assimilatory processes such as the Calvin-Benson cycle are activated under illumination, i.e., under reducing conditions, carbohydrate degradation is switched off during the day. Previous analyses have identified enzymes of the oxidative pentose phosphate pathway to be inactivated by reduction through thioredoxins. In this work, we present evidence that an enzyme of the plastidic glycolysis, the phosphofructokinase isoform AtPFK5, is also inactivated through reduction by thioredoxins, namely by thioredoxin-f. With the help of chemical oxidation, mutant analyses and further experiments, the highly conserved motif CXDXXC in AtPFK5 was identified as the target sequence for this regulatory mechanism. However, knocking out this isoform in plants had only very mild effects on plant growth and performance, indicating that the complex primary metabolism in plants can overcome a lack in AtPFK5 activity.
Stichworte
phosphofructokinase; thioredoxin; Arabidopsis thaliana; redox regulation
Erscheinungsjahr
2021
Zeitschriftentitel
Antioxidants
Band
10
Ausgabe
3
Art.-Nr.
401
eISSN
2076-3921
Page URI
https://pub.uni-bielefeld.de/record/2953831

Zitieren

Hess N, Richter S, Liebthal M, Dietz K-J, Mustroph A. The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. Antioxidants. 2021;10(3): 401.
Hess, N., Richter, S., Liebthal, M., Dietz, K. - J., & Mustroph, A. (2021). The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. Antioxidants, 10(3), 401. https://doi.org/10.3390/antiox10030401
Hess, N., Richter, S., Liebthal, M., Dietz, K. - J., and Mustroph, A. (2021). The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. Antioxidants 10:401.
Hess, N., et al., 2021. The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. Antioxidants, 10(3): 401.
N. Hess, et al., “The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation”, Antioxidants, vol. 10, 2021, : 401.
Hess, N., Richter, S., Liebthal, M., Dietz, K.-J., Mustroph, A.: The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. Antioxidants. 10, : 401 (2021).
Hess, Natalia, Richter, Simon, Liebthal, Michael, Dietz, Karl-Josef, and Mustroph, Angelika. “The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation”. Antioxidants 10.3 (2021): 401.
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