Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation

Budde D, Albano G-L, Noll T, Jurkiewicz E (2021)
Biotechnology Progress: e3150.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Budde, Dana; Albano, Gian-Luca; Noll, ThomasUniBi ; Jurkiewicz, Elke
Abstract / Bemerkung
The presence of leachables in biopharmaceutical processes using single-use technologies (SUT) is well known. For the detection and quantification of the latter, extractable studies of SUT are very common nowadays. Although a mixture of compounds is regularly found in extractable studies, research has only been carried out regarding the effect of individual compounds on cell culture and the cumulative effect of a mix of leachables has not been investigated yet. In this study, a set of leachable model compounds (LMCs) was chosen and the effect of the LMCs on a Chinese hamster ovary DG44 cell line producing an IgG antibody was investigated concerning cell growth, cell cycle distribution and productivity. It was shown that even if worst-case concentrations were used, the LMCs solely impact cell growth. Additionally, interaction studies revealed that the inhibiting effect of the mix is lower than the expected cumulative effect. A strong antagonism between the antioxidant butylated hydroxytoluene and the plasticizer Tris(2-ethylhexyl)trimellitate was found using an isobologram analysis. © 2021 The Authors. Biotechnology Progress published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.
Erscheinungsjahr
2021
Zeitschriftentitel
Biotechnology Progress
Art.-Nr.
e3150
eISSN
1520-6033
Page URI
https://pub.uni-bielefeld.de/record/2953580

Zitieren

Budde D, Albano G-L, Noll T, Jurkiewicz E. Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation. Biotechnology Progress. 2021: e3150.
Budde, D., Albano, G. - L., Noll, T., & Jurkiewicz, E. (2021). Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation. Biotechnology Progress, e3150. https://doi.org/10.1002/btpr.3150
Budde, D., Albano, G. - L., Noll, T., and Jurkiewicz, E. (2021). Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation. Biotechnology Progress:e3150.
Budde, D., et al., 2021. Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation. Biotechnology Progress, : e3150.
D. Budde, et al., “Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation”, Biotechnology Progress, 2021, : e3150.
Budde, D., Albano, G.-L., Noll, T., Jurkiewicz, E.: Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation. Biotechnology Progress. : e3150 (2021).
Budde, Dana, Albano, Gian-Luca, Noll, Thomas, and Jurkiewicz, Elke. “Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation”. Biotechnology Progress (2021): e3150.
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