Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy

Schwake M, Pusch M, Kharkovets T, Jentsch TJ (2000)
Journal of Biological Chemistry 275(18): 13343-13348.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 501.00 KB
Autor*in
Schwake, MichaelUniBi ; Pusch, Michael; Kharkovets, Tatjana; Jentsch, Thomas J.
Abstract / Bemerkung
Mutations in either KCNQ2 or KCNQ3 underlie benign familial neonatal convulsions (BFNC), an inherited epilepsy. The corresponding proteins are co-expressed in broad regions of the brain and associate to heteromeric K+ channels. These channels mediate M-type currents that regulate neuronal excitability. We investigated the basis for the increase in currents seen after co-expressing these subunits in Xenopus oocytes. Noise analysis and single channel recordings revealed a conductance of ≈ 18 pS for KCNQ2 and ≈7 pS for KCNQ3. Different conductance levels (ranging from 8 to 22 pS) were seen upon co-expression. Their weighted average is close to that obtained by noise analysis (16 pS). The open probability of heteromeric channels was not increased significantly. Co-expression of both subunits increased the surface expression of KCNQ2 and KCNQ3 by factors of 5 and >10, respectively. A KCNQ2 mutant associated with BFNC that has a truncated cytoplasmic carboxyl terminus did not reach the surface and failed to stimulate KCNQ3 surface expression. By contrast, several BFNC-associated missense mutations in KCNQ2 or KCNQ3 did not alter their surface expression. Thus, the increase in currents seen upon co-expressing KCNQ2 and KCNQ3 is predominantly due to an increase in surface expression, which is dependent on an intact carboxyl terminus.
Erscheinungsjahr
2000
Zeitschriftentitel
Journal of Biological Chemistry
Band
275
Ausgabe
18
Seite(n)
13343-13348
ISSN
00219258
Page URI
https://pub.uni-bielefeld.de/record/2953365

Zitieren

Schwake M, Pusch M, Kharkovets T, Jentsch TJ. Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy. Journal of Biological Chemistry. 2000;275(18):13343-13348.
Schwake, M., Pusch, M., Kharkovets, T., & Jentsch, T. J. (2000). Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy. Journal of Biological Chemistry, 275(18), 13343-13348. https://doi.org/10.1074/jbc.275.18.13343
Schwake, M., Pusch, M., Kharkovets, T., and Jentsch, T. J. (2000). Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy. Journal of Biological Chemistry 275, 13343-13348.
Schwake, M., et al., 2000. Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy. Journal of Biological Chemistry, 275(18), p 13343-13348.
M. Schwake, et al., “Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy”, Journal of Biological Chemistry, vol. 275, 2000, pp. 13343-13348.
Schwake, M., Pusch, M., Kharkovets, T., Jentsch, T.J.: Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy. Journal of Biological Chemistry. 275, 13343-13348 (2000).
Schwake, Michael, Pusch, Michael, Kharkovets, Tatjana, and Jentsch, Thomas J. “Surface Expression and Single Channel Properties of KCNQ2/KCNQ3, M-type K+ Channels Involved in Epilepsy”. Journal of Biological Chemistry 275.18 (2000): 13343-13348.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2021-04-12T07:18:15Z
MD5 Prüfsumme
3d14c052c085001783e88e3531678569

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 10788442
PubMed | Europe PMC

Suchen in

Google Scholar