Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation

Pachnio A, Dietrich S, Klapper W, Humpe A, Schwake M, Sedlacek R, Gramatzki M, Beck C (2006)
Bone Marrow Transplantation 38(2): 157-167.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Pachnio, A; Dietrich, S; Klapper, W; Humpe, A; Schwake, MichaelUniBi ; Sedlacek, R; Gramatzki, M; Beck, C
Abstract / Bemerkung
Graft-versus-host disease (GvHD) caused by alloreactive T cells within the graft is a major drawback of allogeneic BMT, but depletion of T cells leads to higher rates of relapse, opportunistic infections and graft failure. Therefore, selective removal of GvHD-inducing alloreactive T cells might be beneficial. We describe here the separation of alloresponsive T cells, based on carboxyfluorescein succimidyl ester labeling, in vitro allostimulation and FACS-sorting. In vivo effects of the separated cell populations were investigated in the context of allogeneic BMT in murine models: in vitro resting T cells were shown to survive in the allogeneic host and retain immunoreactivity against ‘third-party’ antigens. As demonstrated in two different transplantation models, elimination of proliferating cells significantly reduces GvHD but offers no advantages to using T-cell-depleted bone marrow alone concerning engraftment and tumor control. Transplanting T cells that proliferate in response to tumor antigens in vitro may narrow down the spectrum of antigens recognized by T cells and therefore reduce GvHD while maintaining graft-facilitating function and tumor control. Therefore, selecting tumor-reactive T cells on the basis of their proliferative response in vitro may be beneficial for the recipient, less time consuming than T-cell cloning and still reduce the extent of GvHD.
Erscheinungsjahr
2006
Zeitschriftentitel
Bone Marrow Transplantation
Band
38
Ausgabe
2
Seite(n)
157-167
ISSN
0268-3369
eISSN
1476-5365
Page URI
https://pub.uni-bielefeld.de/record/2953357

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Pachnio A, Dietrich S, Klapper W, et al. Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation. Bone Marrow Transplantation. 2006;38(2):157-167.
Pachnio, A., Dietrich, S., Klapper, W., Humpe, A., Schwake, M., Sedlacek, R., Gramatzki, M., et al. (2006). Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation. Bone Marrow Transplantation, 38(2), 157-167. https://doi.org/10.1038/sj.bmt.1705411
Pachnio, A., Dietrich, S., Klapper, W., Humpe, A., Schwake, M., Sedlacek, R., Gramatzki, M., and Beck, C. (2006). Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation. Bone Marrow Transplantation 38, 157-167.
Pachnio, A., et al., 2006. Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation. Bone Marrow Transplantation, 38(2), p 157-167.
A. Pachnio, et al., “Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation”, Bone Marrow Transplantation, vol. 38, 2006, pp. 157-167.
Pachnio, A., Dietrich, S., Klapper, W., Humpe, A., Schwake, M., Sedlacek, R., Gramatzki, M., Beck, C.: Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation. Bone Marrow Transplantation. 38, 157-167 (2006).
Pachnio, A, Dietrich, S, Klapper, W, Humpe, A, Schwake, Michael, Sedlacek, R, Gramatzki, M, and Beck, C. “Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation”. Bone Marrow Transplantation 38.2 (2006): 157-167.

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