Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology

Romanova N, Niemann T, Greiner J, Kaltschmidt B, Kaltschmidt C, Noll T (2021)
Biotechnology and Bioengineering 118(6): 2348-2359.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Exposure of Chinese hamster ovary cells (CHO) to highly concentrated feed solution during fed‐batch cultivation is known to result in an unphysiological osmolality increase (>300 mOsm/kg), affecting cell physiology and morphology. Extending previous observation on osmotic adaptation, the present study investigates for the first time potential effects of hyperosmolality on CHO cells on both population and single‐cell level. We intentionally exposed CHO cells to hyperosmolality of up to 545 mOsm/kg during fed‐batch cultivation. In concordance with existing research data, hyperosmolality‐exposed CHO cells showed a nearly triplicated volume accompanied by ablation of proliferation. On the molecular level, we observed a strong hyperosmolality‐dependent increase in mitochondrial activity in CHO cells compared to control. In contrast to mitochondrial activity, hyperosmolality‐dependent proliferation arrest of CHO cells was not accompanied by DNA accumulation or caspase‐3/7‐mediated apoptosis. Notably, we demonstrate for the first time a formation of up to eight multiple, small nuclei in single hyperosmolality‐stressed CHO cells. The here presented observations reveal previously unknown hyperosmolality‐dependent morphological changes in CHO cells and support existing data on the osmotic response in mammalian cells.
Erscheinungsjahr
2021
Zeitschriftentitel
Biotechnology and Bioengineering
Band
118
Ausgabe
6
Seite(n)
2348-2359
ISSN
0006-3592
eISSN
1097-0290
Page URI
https://pub.uni-bielefeld.de/record/2952639

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Romanova N, Niemann T, Greiner J, Kaltschmidt B, Kaltschmidt C, Noll T. Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering. 2021;118(6):2348-2359.
Romanova, N., Niemann, T., Greiner, J., Kaltschmidt, B., Kaltschmidt, C., & Noll, T. (2021). Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering, 118(6), 2348-2359. https://doi.org/10.1002/bit.27747
Romanova, N., Niemann, T., Greiner, J., Kaltschmidt, B., Kaltschmidt, C., and Noll, T. (2021). Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering 118, 2348-2359.
Romanova, N., et al., 2021. Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering, 118(6), p 2348-2359.
N. Romanova, et al., “Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology”, Biotechnology and Bioengineering, vol. 118, 2021, pp. 2348-2359.
Romanova, N., Niemann, T., Greiner, J., Kaltschmidt, B., Kaltschmidt, C., Noll, T.: Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering. 118, 2348-2359 (2021).
Romanova, Nadiya, Niemann, Tarek, Greiner, Johannes, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Noll, Thomas. “Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology”. Biotechnology and Bioengineering 118.6 (2021): 2348-2359.
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