Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology

Romanova N, Niemann T, Greiner J, Kaltschmidt B, Kaltschmidt C, Noll T (2021)
Biotechnology and Bioengineering 118(6): 2348-2359.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 4.19 MB
Abstract / Bemerkung
Exposure of Chinese hamster ovary cells (CHO) to highly concentrated feed solution during fed‐batch cultivation is known to result in an unphysiological osmolality increase (>300 mOsm/kg), affecting cell physiology and morphology. Extending previous observation on osmotic adaptation, the present study investigates for the first time potential effects of hyperosmolality on CHO cells on both population and single‐cell level. We intentionally exposed CHO cells to hyperosmolality of up to 545 mOsm/kg during fed‐batch cultivation. In concordance with existing research data, hyperosmolality‐exposed CHO cells showed a nearly triplicated volume accompanied by ablation of proliferation. On the molecular level, we observed a strong hyperosmolality‐dependent increase in mitochondrial activity in CHO cells compared to control. In contrast to mitochondrial activity, hyperosmolality‐dependent proliferation arrest of CHO cells was not accompanied by DNA accumulation or caspase‐3/7‐mediated apoptosis. Notably, we demonstrate for the first time a formation of up to eight multiple, small nuclei in single hyperosmolality‐stressed CHO cells. The here presented observations reveal previously unknown hyperosmolality‐dependent morphological changes in CHO cells and support existing data on the osmotic response in mammalian cells.
Erscheinungsjahr
2021
Zeitschriftentitel
Biotechnology and Bioengineering
Band
118
Ausgabe
6
Seite(n)
2348-2359
ISSN
0006-3592
eISSN
1097-0290
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld im Rahmen des DEAL-Vertrags gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2952639

Zitieren

Romanova N, Niemann T, Greiner J, Kaltschmidt B, Kaltschmidt C, Noll T. Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering. 2021;118(6):2348-2359.
Romanova, N., Niemann, T., Greiner, J., Kaltschmidt, B., Kaltschmidt, C., & Noll, T. (2021). Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering, 118(6), 2348-2359. https://doi.org/10.1002/bit.27747
Romanova, Nadiya, Niemann, Tarek, Greiner, Johannes, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Noll, Thomas. 2021. “Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology”. Biotechnology and Bioengineering 118 (6): 2348-2359.
Romanova, N., Niemann, T., Greiner, J., Kaltschmidt, B., Kaltschmidt, C., and Noll, T. (2021). Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering 118, 2348-2359.
Romanova, N., et al., 2021. Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering, 118(6), p 2348-2359.
N. Romanova, et al., “Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology”, Biotechnology and Bioengineering, vol. 118, 2021, pp. 2348-2359.
Romanova, N., Niemann, T., Greiner, J., Kaltschmidt, B., Kaltschmidt, C., Noll, T.: Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology. Biotechnology and Bioengineering. 118, 2348-2359 (2021).
Romanova, Nadiya, Niemann, Tarek, Greiner, Johannes, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Noll, Thomas. “Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology”. Biotechnology and Bioengineering 118.6 (2021): 2348-2359.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International (CC BY-NC-ND 4.0):
Volltext(e)
Name
Access Level
OA Open Access
Zuletzt Hochgeladen
2021-07-19T08:53:12Z
MD5 Prüfsumme
76d4f31eaa450e3e57e9abcf3087919c


Link(s) zu Volltext(en)
Access Level
OA Open Access

Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

References

Daten bereitgestellt von Europe PubMed Central.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 33751545
PubMed | Europe PMC

Suchen in

Google Scholar