Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions

Zwiener T, Dziuba M, Mickoleit F, Rückert C, Busche T, Kalinowski J, Uebe R, Schueler D (2021)
Microbial Cell Factories 20(1): 35.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Zwiener, Theresa; Dziuba, Marina; Mickoleit, Frank; Rückert, ChristianUniBi ; Busche, TobiasUniBi; Kalinowski, JörnUniBi; Uebe, Rene; Schueler, Dirk
Abstract / Bemerkung
Background: Because of its tractability and straightforward cultivation, the magnetic bacterium Magnetospirillum gryphiswaldense has emerged as a model for the analysis of magnetosome biosynthesis and bioproduction. However, its future use as platform for synthetic biology and biotechnology will require methods for large-scale genome editing and streamlining. Results: We established an approach for combinatory genome reduction and generated a library of strains in which up to 16 regions including large gene clusters, mobile genetic elements and phage-related genes were sequentially removed, equivalent to similar to 227.6 kb and nearly 5.5% of the genome. Finally, the fragmented genomic magnetosome island was replaced by a compact cassette comprising all key magnetosome biosynthetic gene clusters. The prospective 'chassis' revealed wild type-like cell growth and magnetosome biosynthesis under optimal conditions, as well as slightly improved resilience and increased genetic stability. Conclusion: We provide first proof-of-principle for the feasibility of multiple genome reduction and large-scale engineering of magnetotactic bacteria. The library of deletions will be valuable for turning M. gryphiswaldense into a microbial cell factory for synthetic biology and production of magnetic nanoparticles.
Stichworte
Magnetospirillum gryphiswaldense; Magnetotactic bacteria; Magnetosomes; Genome reduction; Chassis; IS elements
Erscheinungsjahr
2021
Zeitschriftentitel
Microbial Cell Factories
Band
20
Ausgabe
1
Art.-Nr.
35
eISSN
1475-2859
Page URI
https://pub.uni-bielefeld.de/record/2952132

Zitieren

Zwiener T, Dziuba M, Mickoleit F, et al. Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions. Microbial Cell Factories. 2021;20(1): 35.
Zwiener, T., Dziuba, M., Mickoleit, F., Rückert, C., Busche, T., Kalinowski, J., Uebe, R., et al. (2021). Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions. Microbial Cell Factories, 20(1), 35. https://doi.org/10.1186/s12934-021-01517-2
Zwiener, T., Dziuba, M., Mickoleit, F., Rückert, C., Busche, T., Kalinowski, J., Uebe, R., and Schueler, D. (2021). Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions. Microbial Cell Factories 20:35.
Zwiener, T., et al., 2021. Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions. Microbial Cell Factories, 20(1): 35.
T. Zwiener, et al., “Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions”, Microbial Cell Factories, vol. 20, 2021, : 35.
Zwiener, T., Dziuba, M., Mickoleit, F., Rückert, C., Busche, T., Kalinowski, J., Uebe, R., Schueler, D.: Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions. Microbial Cell Factories. 20, : 35 (2021).
Zwiener, Theresa, Dziuba, Marina, Mickoleit, Frank, Rückert, Christian, Busche, Tobias, Kalinowski, Jörn, Uebe, Rene, and Schueler, Dirk. “Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions”. Microbial Cell Factories 20.1 (2021): 35.

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