Function and Regulation of Chloroplast Peroxiredoxin IIE
Dreyer A, Treffon P, Basiry D, Jozefowicz AM, Matros A, Mock H-P, Dietz K-J (2021)
Antioxidants 10(2): 152.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
![Open access file OA](https://pub.uni-bielefeld.de/images/access_open.png)
Autor*in
Dreyer, AnnaUniBi
;
Treffon, PatrickUniBi;
Basiry, Daniel;
Jozefowicz, Anna Maria;
Matros, Andrea;
Mock, Hans-Peter;
Dietz, Karl-JosefUniBi
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Abstract / Bemerkung
Peroxiredoxins (PRX) are thiol peroxidases that are highly conserved throughout all biological kingdoms. Increasing evidence suggests that their high reactivity toward peroxides has a function not only in antioxidant defense but in particular in redox regulation of the cell. Peroxiredoxin IIE (PRX-IIE) is one of three PRX types found in plastids and has previously been linked to pathogen defense and protection from protein nitration. However, its posttranslational regulation and its function in the chloroplast protein network remained to be explored. Using recombinant protein, it was shown that the peroxidatic Cys121 is subjected to multiple posttranslational modifications, namely disulfide formation, S-nitrosation, S-glutathionylation, and hyperoxidation. Slightly oxidized glutathione fostered S-glutathionylation and inhibited activity in vitro. Immobilized recombinant PRX-IIE allowed trapping and subsequent identification of interaction partners by mass spectrometry. Interaction with the 14-3-3 υ protein was confirmed in vitro and was shown to be stimulated under oxidizing conditions. Interactions did not depend on phosphorylation as revealed by testing phospho-mimicry variants of PRX-IIE. Based on these data it is proposed that 14-3-3υ guides PRX‑IIE to certain target proteins, possibly for redox regulation. These findings together with the other identified potential interaction partners of type II PRXs localized to plastids, mitochondria, and cytosol provide a new perspective on the redox regulatory network of the cell.
Erscheinungsjahr
2021
Zeitschriftentitel
Antioxidants
Band
10
Ausgabe
2
Art.-Nr.
152
Urheberrecht / Lizenzen
eISSN
2076-3921
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2950341
Zitieren
Dreyer A, Treffon P, Basiry D, et al. Function and Regulation of Chloroplast Peroxiredoxin IIE. Antioxidants. 2021;10(2): 152.
Dreyer, A., Treffon, P., Basiry, D., Jozefowicz, A. M., Matros, A., Mock, H. - P., & Dietz, K. - J. (2021). Function and Regulation of Chloroplast Peroxiredoxin IIE. Antioxidants, 10(2), 152. https://doi.org/10.3390/antiox10020152
Dreyer, Anna, Treffon, Patrick, Basiry, Daniel, Jozefowicz, Anna Maria, Matros, Andrea, Mock, Hans-Peter, and Dietz, Karl-Josef. 2021. “Function and Regulation of Chloroplast Peroxiredoxin IIE”. Antioxidants 10 (2): 152.
Dreyer, A., Treffon, P., Basiry, D., Jozefowicz, A. M., Matros, A., Mock, H. - P., and Dietz, K. - J. (2021). Function and Regulation of Chloroplast Peroxiredoxin IIE. Antioxidants 10:152.
Dreyer, A., et al., 2021. Function and Regulation of Chloroplast Peroxiredoxin IIE. Antioxidants, 10(2): 152.
A. Dreyer, et al., “Function and Regulation of Chloroplast Peroxiredoxin IIE”, Antioxidants, vol. 10, 2021, : 152.
Dreyer, A., Treffon, P., Basiry, D., Jozefowicz, A.M., Matros, A., Mock, H.-P., Dietz, K.-J.: Function and Regulation of Chloroplast Peroxiredoxin IIE. Antioxidants. 10, : 152 (2021).
Dreyer, Anna, Treffon, Patrick, Basiry, Daniel, Jozefowicz, Anna Maria, Matros, Andrea, Mock, Hans-Peter, and Dietz, Karl-Josef. “Function and Regulation of Chloroplast Peroxiredoxin IIE”. Antioxidants 10.2 (2021): 152.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Name
antioxidants-10-00152.dietz.pdf
2.31 MB
Access Level
![Open access file OA](https://pub.uni-bielefeld.de/images/access_open.png)
Zuletzt Hochgeladen
2021-01-21T09:38:15Z
MD5 Prüfsumme
3d2b03d03d8d28551d718b20a32bbfb0
Daten bereitgestellt von European Bioinformatics Institute (EBI)
Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
References
Daten bereitgestellt von Europe PubMed Central.
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 33494157
PubMed | Europe PMC
Suchen in