Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum

Henke NA, Krahn I, Wendisch VF (2021)
Microorganisms 9(1): 204.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 124.99 KB
Abstract / Bemerkung
Corynebacterium glutamicum has been safely used in white biotechnology for the last 60 years and the portfolio of new pathways and products is increasing rapidly. Hence, expression vectors play a central role in discovering endogenous gene functions and in establishing heterologous gene expression. In this work, new expression vectors were designed based on two strategies: (i) a library screening of constitutive native and synthetic promoters and (ii) an increase of the plasmid copy number. Both strategies were combined and resulted in a very strong expression and overproduction of the fluorescence protein GfpUV. As a second test case, the improved vector for constitutive expression was used to overexpress the endogenous xylulokinase gene xylB in a synthetic operon with xylose isomerase gene xylA from Xanthomonas campestris. The xylose isomerase activity in crude extracts was increased by about three-fold as compared to that of the parental vector. In terms of application, the improved vector for constitutive xylA and xylB expression was used for production of the N-methylated amino acid sarcosine from monomethylamine, acetate, and xylose. As a consequence, the volumetric productivity of sarcosine production was 50% higher as compared to that of the strain carrying the parental vector.
Stichworte
expression vector; Corynebacterium glutamicum; overexpression; promoter; origin of replication
Erscheinungsjahr
2021
Zeitschriftentitel
Microorganisms
Band
9
Ausgabe
1
Art.-Nr.
204
eISSN
2076-2607
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2950258

Zitieren

Henke NA, Krahn I, Wendisch VF. Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum. Microorganisms. 2021;9(1): 204.
Henke, N. A., Krahn, I., & Wendisch, V. F. (2021). Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum. Microorganisms, 9(1), 204. doi:10.3390/microorganisms9010204
Henke, N. A., Krahn, I., and Wendisch, V. F. (2021). Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum. Microorganisms 9:204.
Henke, N.A., Krahn, I., & Wendisch, V.F., 2021. Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum. Microorganisms, 9(1): 204.
N.A. Henke, I. Krahn, and V.F. Wendisch, “Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum”, Microorganisms, vol. 9, 2021, : 204.
Henke, N.A., Krahn, I., Wendisch, V.F.: Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum. Microorganisms. 9, : 204 (2021).
Henke, Nadja Alina, Krahn, Irene, and Wendisch, Volker F. “Improved plasmid-based inducible and constitutive gene expression in Corynebacterium glutamicum”. Microorganisms 9.1 (2021): 204.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2021-01-22T06:52:12Z
MD5 Prüfsumme
11ca6505a121c44e367ddd45a399131b

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 33478126
PubMed | Europe PMC

Suchen in

Google Scholar