A universal method for gene expression engineering
Lale R, Tietze L, Nesje J, Onsager I, Engelhardt K, Wong CFA, Akan M, Hummel N, Kalinowski J, Rückert C, Hohmann-Marriott MF (Unpublished)
bioRxiv.
Preprint
| Unveröffentlicht | Englisch
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Autor*in
Lale, Rahmi;
Tietze, Lisa;
Nesje, Jenny;
Onsager, Ingerid;
Engelhardt, Kerstin;
Wong, Che Fai Alex;
Akan, Madina;
Hummel, Niklas;
Kalinowski, JörnUniBi;
Rückert, ChristianUniBi 
;
Hohmann-Marriott, Martin Frank
;
Hohmann-Marriott, Martin FrankAbstract / Bemerkung
The precise expression of genes is one of the foundations of biotechnology. Here we present GeneEE, a straightforward method for generating artificial gene expression systems. We demonstrate that GeneEE segments, containing a 200 nucleotide DNA with random nucleotide composition, can facilitate constitutive and inducible gene expression. To highlight the universal character of our method, we demonstrate GeneEE-mediated gene and protein expression in six bacterial species and Baker’s yeast.
Erscheinungsjahr
2019
Zeitschriftentitel
bioRxiv
Page URI
https://pub.uni-bielefeld.de/record/2949925
Zitieren
Lale R, Tietze L, Nesje J, et al. A universal method for gene expression engineering. bioRxiv. Unpublished.
Lale, R., Tietze, L., Nesje, J., Onsager, I., Engelhardt, K., Wong, C. F. A., Akan, M., et al. (Unpublished). A universal method for gene expression engineering. bioRxiv. doi:10.1101/644989
Lale, Rahmi, Tietze, Lisa, Nesje, Jenny, Onsager, Ingerid, Engelhardt, Kerstin, Wong, Che Fai Alex, Akan, Madina, et al. Unpublished. “A universal method for gene expression engineering”. bioRxiv.
Lale, R., Tietze, L., Nesje, J., Onsager, I., Engelhardt, K., Wong, C. F. A., Akan, M., Hummel, N., Kalinowski, J., Rückert, C., et al. (Unpublished). A universal method for gene expression engineering. bioRxiv.
Lale, R., et al., Unpublished. A universal method for gene expression engineering. bioRxiv.
R. Lale, et al., “A universal method for gene expression engineering”, bioRxiv, Unpublished.
Lale, R., Tietze, L., Nesje, J., Onsager, I., Engelhardt, K., Wong, C.F.A., Akan, M., Hummel, N., Kalinowski, J., Rückert, C., Hohmann-Marriott, M.F.: A universal method for gene expression engineering. bioRxiv. (Unpublished).
Lale, Rahmi, Tietze, Lisa, Nesje, Jenny, Onsager, Ingerid, Engelhardt, Kerstin, Wong, Che Fai Alex, Akan, Madina, Hummel, Niklas, Kalinowski, Jörn, Rückert, Christian, and Hohmann-Marriott, Martin Frank. “A universal method for gene expression engineering”. bioRxiv (Unpublished).
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