A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus.

Gad D, Elhaak M, Pompa A, Mattar M, Zayed M, Fraternale D, Dietz K-J (2020)
Genes 11(7): 791.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Gad, DinaUniBi; Elhaak, Mahmoud; Pompa, Andrea; Mattar, Magdy; Zayed, Mohamed; Fraternale, Daniele; Dietz, Karl-JosefUniBi
Abstract / Bemerkung
There is a need to enhance the production of bioactive secondary metabolites and to establish new production systems, e.g., for liver-protective compounds of Silybum marianum seeds. Quantifying and identifying the produced phytochemicals, and examining their protective effects against genotoxic agents, is of great interest. This study established a protocol for the qualitative and quantitative production of hepatoprotective compounds in cotyledon-derived Silybum marianum callus through optimized supplementation of the MS medium with the growth regulators 2,4-D, benzylaminopurine, myoinositol, and asparagine. High-performance liquid chromatography (HPLC) coupled with electrospray ionisation mass spectrometry (ESI-MS) allowed for identification and quantification of the produced compounds. None of the growth medium combinations supported a detectable production of silymarin. Instead, the generated calli accumulated phenolic acids, in particular chlorogenic acid and dicaffeoylquinic acid, as revealed by HPLC and mass spectrometric analysis. 4-Nitro-o-phenylenediamine (NPD) was employed in the AMES-test with Salmonella typhimurium strain TA98 because it is a potent mutagen for this strain. Results revealed that callus extract had a high anti-genotoxic activity with respect to standard silymarin but more evident with respect seed extract. The callus produced chlorogenic acid and dicaffeoylquinic acid, which revealed higher bioactivity than silymarin. Both compounds were not formed or could not be detected in the seeds of Silybum marianum Egyptian ecotype.
Erscheinungsjahr
2020
Zeitschriftentitel
Genes
Band
11
Ausgabe
7
Art.-Nr.
791
eISSN
2073-4425
Page URI
https://pub.uni-bielefeld.de/record/2944991

Zitieren

Gad D, Elhaak M, Pompa A, et al. A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus. Genes. 2020;11(7): 791.
Gad, D., Elhaak, M., Pompa, A., Mattar, M., Zayed, M., Fraternale, D., & Dietz, K. - J. (2020). A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus. Genes, 11(7), 791. doi:10.3390/genes11070791
Gad, D., Elhaak, M., Pompa, A., Mattar, M., Zayed, M., Fraternale, D., and Dietz, K. - J. (2020). A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus. Genes 11:791.
Gad, D., et al., 2020. A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus. Genes, 11(7): 791.
D. Gad, et al., “A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus.”, Genes, vol. 11, 2020, : 791.
Gad, D., Elhaak, M., Pompa, A., Mattar, M., Zayed, M., Fraternale, D., Dietz, K.-J.: A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus. Genes. 11, : 791 (2020).
Gad, Dina, Elhaak, Mahmoud, Pompa, Andrea, Mattar, Magdy, Zayed, Mohamed, Fraternale, Daniele, and Dietz, Karl-Josef. “A New Strategy to Increase Production of Genoprotective Bioactive Molecules from Cotyledon-Derived Silybum marianum L. Callus.”. Genes 11.7 (2020): 791.

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