A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping

Holtgräwe D, Rosleff Soerensen T, Hausmann L, Pucker B, Viehöver P, Töpfer R, Weisshaar B (2020)
Frontiers in Plant Science 11: 156.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Holtgräwe, DanielaUniBi ; Rosleff Soerensen, Thomas; Hausmann, Ludger; Pucker, BoasUniBi ; Viehöver, PriscaUniBi; Töpfer, Reinhard; Weisshaar, BerndUniBi
Abstract / Bemerkung
Grapevine breeding has become highly relevant due to upcoming challenges like climate change, a decrease in the number of available fungicides, increasing public concern about plant protection, and the demand for a sustainable production. Downy mildew caused by Plasmopara viticola is one of the most devastating diseases worldwide of cultivated Vitis vinifera. In modern breeding programs, therefore, genetic marker technologies and genomic data are used to develop new cultivars with defined and stacked resistance loci. Potential sources of resistance are wild species of American or Asian origin. The interspecific hybrid of Vitis riparia Gm 183 x Vitis cinerea Arnold, available as the rootstock cultivar ‘Börner,’ carries several relevant resistance loci. We applied next-generation sequencing to enable the reliable identification of simple sequence repeats (SSR), and we also generated a draft genome sequence assembly of ‘Börner’ to access genome-wide sequence variations in a comprehensive and highly reliable way. These data were used to cover the ‘Börner’ genome with genetic marker positions. A subset of these marker positions was used for targeted mapping of the P. viticola resistance locus, Rpv14, to validate the marker position list. Based on the reference genome sequence PN40024, the position of this resistance locus can be narrowed down to less than 0.5 Mbp on chromosome 5.
Stichworte
Plant Science
Erscheinungsjahr
2020
Zeitschriftentitel
Frontiers in Plant Science
Band
11
Art.-Nr.
156
eISSN
1664-462X
Page URI
https://pub.uni-bielefeld.de/record/2941655

Zitieren

Holtgräwe D, Rosleff Soerensen T, Hausmann L, et al. A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping. Frontiers in Plant Science. 2020;11: 156.
Holtgräwe, D., Rosleff Soerensen, T., Hausmann, L., Pucker, B., Viehöver, P., Töpfer, R., & Weisshaar, B. (2020). A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping. Frontiers in Plant Science, 11, 156. doi:10.3389/fpls.2020.00156
Holtgräwe, D., Rosleff Soerensen, T., Hausmann, L., Pucker, B., Viehöver, P., Töpfer, R., and Weisshaar, B. (2020). A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping. Frontiers in Plant Science 11:156.
Holtgräwe, D., et al., 2020. A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping. Frontiers in Plant Science, 11: 156.
D. Holtgräwe, et al., “A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping”, Frontiers in Plant Science, vol. 11, 2020, : 156.
Holtgräwe, D., Rosleff Soerensen, T., Hausmann, L., Pucker, B., Viehöver, P., Töpfer, R., Weisshaar, B.: A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping. Frontiers in Plant Science. 11, : 156 (2020).
Holtgräwe, Daniela, Rosleff Soerensen, Thomas, Hausmann, Ludger, Pucker, Boas, Viehöver, Prisca, Töpfer, Reinhard, and Weisshaar, Bernd. “A Partially Phase-Separated Genome Sequence Assembly of the Vitis Rootstock ‘Börner’ (Vitis riparia × Vitis cinerea) and Its Exploitation for Marker Development and Targeted Mapping”. Frontiers in Plant Science 11 (2020): 156.
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Material in PUB:
Teil von PUB Eintrag
Heterozygous SNVs between BoeWGS1.0 and PN40024
Holtgräwe D, Pucker B, Rosleff Sörensen T, Weisshaar B (2019)
Bielefeld University.
Teil von PUB Eintrag
Homozygous SNVs between BoeWGS1.0 and PN40024
Holtgräwe D, Pucker B, Rosleff Sörensen T, Weisshaar B (2019)
Bielefeld University.
Teil von PUB Eintrag
Description and mapping positions of BoeWGS1.0 contigs
Holtgräwe D, Pucker B, Rosleff Sörensen T, Weisshaar B (2019)
Bielefeld University.

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PMID: 32194587
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Preprint: 10.1101/854687

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