Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate

Graf M, Haas T, Mueller F, Buchmann A, Harm-Bekbenbetova J, Freund A, Niess A, Persicke M, Kalinowski J, Blombach B, Takors R (2019)
Frontiers in Microbiology 10: 1648.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Corynebacterium glutamicum is a commonly applied host for the industrial production of amino acids. While valued for its robustness, it is somewhat inferior to competing strains such as Escherichia coli because of the relatively low growth rate of 0.40 h(-1) in synthetic, industrial media. Accordingly, adaptive laboratory evolution (ALE) experiments were performed in continuous cultivation mode to select for a growth-improved host. To ensure industrial attractiveness, this ALE study aimed at a reduction of dependency on costly growth-boosting additives such as protocatechuate (RCA) or complex media supplements. Consequently, double selection pressures were installed consisting of a steady increase in growth rate demands and a parallel reduction of complex medium fractions. Selection yielded C. glutamicum EVO5 achieving 0.54 h(-1) and 1.03g(Glc) g(CDW)(-1) h(-1) in minimal medium without above mentioned supplements. Sequencing revealed 10 prominent mutations, three of them in key regulator genes.
Stichworte
Corynebacterium glutamicum; adaptive laboratory evolution; continuous; adaptive evolution; increased growth; protocatechuate; RamA; DtxR; RpoA
Erscheinungsjahr
2019
Zeitschriftentitel
Frontiers in Microbiology
Band
10
Art.-Nr.
1648
eISSN
1664-302X
Page URI
https://pub.uni-bielefeld.de/record/2936922

Zitieren

Graf M, Haas T, Mueller F, et al. Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate. Frontiers in Microbiology . 2019;10: 1648.
Graf, M., Haas, T., Mueller, F., Buchmann, A., Harm-Bekbenbetova, J., Freund, A., Niess, A., et al. (2019). Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate. Frontiers in Microbiology , 10, 1648. doi:10.3389/fmicb.2019.01648
Graf, M., Haas, T., Mueller, F., Buchmann, A., Harm-Bekbenbetova, J., Freund, A., Niess, A., Persicke, M., Kalinowski, J., Blombach, B., et al. (2019). Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate. Frontiers in Microbiology 10:1648.
Graf, M., et al., 2019. Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate. Frontiers in Microbiology , 10: 1648.
M. Graf, et al., “Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate”, Frontiers in Microbiology , vol. 10, 2019, : 1648.
Graf, M., Haas, T., Mueller, F., Buchmann, A., Harm-Bekbenbetova, J., Freund, A., Niess, A., Persicke, M., Kalinowski, J., Blombach, B., Takors, R.: Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate. Frontiers in Microbiology . 10, : 1648 (2019).
Graf, Michaela, Haas, Thorsten, Mueller, Felix, Buchmann, Anina, Harm-Bekbenbetova, Julia, Freund, Andreas, Niess, Alexander, Persicke, Marcus, Kalinowski, Jörn, Blombach, Bastian, and Takors, Ralf. “Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate”. Frontiers in Microbiology 10 (2019): 1648.

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