Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells.

Buß M, Tegtmeyer N, Schnieder J, Dong X, Li J, Springer TA, Backert S, Niemann H (2019)
The FEBS journal.

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Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
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CagL is an essential pilus surface component of the virulence-associated type IV secretion system (T4SS) employed by Helicobacterpylori to translocate the oncogenic effector protein CagA into human gastric epithelial cells. CagL contains an RGD motif and integrin alpha5 beta1 is widely accepted as its host cell receptor. Here, we show that CagL binds integrin alphaV beta6 with substantially higher affinity and that this interaction is functionally important. Cell surface expression of alphaV beta6 on various cell lines correlated perfectly with cell adhesion to immobilized CagL and with binding of soluble CagL to cells. We found no such correlation for alpha5 beta1 . The purified alphaV beta6 ectodomain bound CagL with high affinity. This interaction was highly specific, as the affinity of CagL for other RGD-binding integrins was two to three orders of magnitude weaker. Mutation of either conserved leucine in the CagL RGDLXXL motif, a motif that generally confers specificity for integrin alphaV beta6 and alphaV beta8 , lowered the affinity of CagL for alphaV beta6 . Stable expression of alphaV beta6 in alphaV beta6 -negative but alpha5 beta1 -expressing human cells promoted two hallmarks of the functional H.pylori T4SS, namely translocation of CagA into host cells and induction of interleukin-8 secretion by host cells. These findings suggest that integrin alphaV beta6 , although not essential for T4SS function, represents an important host cell receptor for CagL. © 2019 Federation of European Biochemical Societies.
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Buß M, Tegtmeyer N, Schnieder J, et al. Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells. The FEBS journal. 2019.
Buß, M., Tegtmeyer, N., Schnieder, J., Dong, X., Li, J., Springer, T. A., Backert, S., et al. (2019). Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells. The FEBS journal. doi:10.1111/febs.14962
Buß, M., Tegtmeyer, N., Schnieder, J., Dong, X., Li, J., Springer, T. A., Backert, S., and Niemann, H. (2019). Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells. The FEBS journal.
Buß, M., et al., 2019. Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells. The FEBS journal.
M. Buß, et al., “Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells.”, The FEBS journal, 2019.
Buß, M., Tegtmeyer, N., Schnieder, J., Dong, X., Li, J., Springer, T.A., Backert, S., Niemann, H.: Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells. The FEBS journal. (2019).
Buß, Maren, Tegtmeyer, Nicole, Schnieder, Jennifer, Dong, Xianchi, Li, Jing, Springer, Timothy A, Backert, Steffen, and Niemann, Hartmut. “Specific high affinity interaction of Helicobacterpylori CagL with integrin alphaV beta6 promotes type IV secretion of CagA into human cells.”. The FEBS journal (2019).

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PMID: 31197920
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