Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2

Koshla OT, Rokytskyy IV, Ostash IS, Busche T, Kalinowski J, Moesker E, Suessmuth RD, Fedorenko VO, Ostash BO (2019)
CYTOLOGY AND GENETICS 53(1): 1-7.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Streptomyces albus J1074 is one of the most popular chassis for heterologous expression of actino-bacterial biosynthetic gene clusters (BGCs). Considerable efforts are invested into understanding all the physiological and genetic aspects of secondary metabolism of J1074 in order to maximize the expression of heterologous BGCs. It has to be noted that the J1074 genome itself is home to numerous (> 20) BGCs, whose expression varies widely. Therefore, the identification of the factors limiting the expression of J1074 BGCs might help improve this strain for heterologous expression purposes. As first steps towards this goal, herein we describe the secondary metabolome of J1074 in liquid medium SG2, previously shown by us to support the production of antibacterial and antifungal compounds. We compare the results of metabolomic studies with the transcriptome of J1074 in SG2 after 60 h of growth. Results of our studies are discussed in the context of current knowledge on the J1074 transcriptome and metabolome data.
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Zeitschriftentitel
CYTOLOGY AND GENETICS
Band
53
Ausgabe
1
Seite(n)
1-7
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Koshla OT, Rokytskyy IV, Ostash IS, et al. Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2. CYTOLOGY AND GENETICS. 2019;53(1):1-7.
Koshla, O. T., Rokytskyy, I. V., Ostash, I. S., Busche, T., Kalinowski, J., Moesker, E., Suessmuth, R. D., et al. (2019). Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2. CYTOLOGY AND GENETICS, 53(1), 1-7. doi:10.3103/S0095452719010080
Koshla, O. T., Rokytskyy, I. V., Ostash, I. S., Busche, T., Kalinowski, J., Moesker, E., Suessmuth, R. D., Fedorenko, V. O., and Ostash, B. O. (2019). Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2. CYTOLOGY AND GENETICS 53, 1-7.
Koshla, O.T., et al., 2019. Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2. CYTOLOGY AND GENETICS, 53(1), p 1-7.
O.T. Koshla, et al., “Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2”, CYTOLOGY AND GENETICS, vol. 53, 2019, pp. 1-7.
Koshla, O.T., Rokytskyy, I.V., Ostash, I.S., Busche, T., Kalinowski, J., Moesker, E., Suessmuth, R.D., Fedorenko, V.O., Ostash, B.O.: Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2. CYTOLOGY AND GENETICS. 53, 1-7 (2019).
Koshla, O. T., Rokytskyy, I. V., Ostash, I. S., Busche, Tobias, Kalinowski, Jörn, Moesker, E., Suessmuth, R. D., Fedorenko, V. O., and Ostash, B. O. “Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2”. CYTOLOGY AND GENETICS 53.1 (2019): 1-7.