Secretome Dynamics in a Gram-Positive Bacterial Model

Tsolis KC, Hamed MB, Simoens K, Koepff J, Busche T, Rückert C, Oldiges M, Kalinowski J, Anne J, Kormanec J, Bernaerts K, et al. (2019)
MOLECULAR & CELLULAR PROTEOMICS 18(3): 423-436.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Protein secretion is a central biological process in all organisms. Most studies dissecting bacterial secretion mechanisms have focused on Gram-negative cell envelopes such as that of Escherichia coli. However, proteomics analyses in Gram negatives is hampered by their outer membrane. Here we studied protein secretion in the Gram-positive bacterium Streptomyces lividans TK24, in which most of the secretome is released in the growth medium. We monitored changes of the secretome as a function of growth phase and medium. We determined distinct protein classes of "house-keeping" secreted proteins that do not change their appearance or abundance in the various media and growth phases. These comprise mainly enzymes involved in cell wall maintenance and basic transport. In addition, we detected significant abundance and content changes to a sub-set of the proteome, as a function of growth in the different media. These did not depend on the media being minimal or rich. Transcriptional regulation but not changes in export machinery components can explain some of these changes. However, additional downstream mechanisms must be important for selective secretome funneling. These observations lay the foundations of using S. lividans as a model organism to study how metabolism is linked to optimal secretion and help develop rational optimization of heterologous protein production.
Erscheinungsjahr
Zeitschriftentitel
MOLECULAR & CELLULAR PROTEOMICS
Band
18
Ausgabe
3
Seite(n)
423-436
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Tsolis KC, Hamed MB, Simoens K, et al. Secretome Dynamics in a Gram-Positive Bacterial Model. MOLECULAR & CELLULAR PROTEOMICS. 2019;18(3):423-436.
Tsolis, K. C., Hamed, M. B., Simoens, K., Koepff, J., Busche, T., Rückert, C., Oldiges, M., et al. (2019). Secretome Dynamics in a Gram-Positive Bacterial Model. MOLECULAR & CELLULAR PROTEOMICS, 18(3), 423-436. doi:10.1074/mcp.RA118.000899
Tsolis, K. C., Hamed, M. B., Simoens, K., Koepff, J., Busche, T., Rückert, C., Oldiges, M., Kalinowski, J., Anne, J., Kormanec, J., et al. (2019). Secretome Dynamics in a Gram-Positive Bacterial Model. MOLECULAR & CELLULAR PROTEOMICS 18, 423-436.
Tsolis, K.C., et al., 2019. Secretome Dynamics in a Gram-Positive Bacterial Model. MOLECULAR & CELLULAR PROTEOMICS, 18(3), p 423-436.
K.C. Tsolis, et al., “Secretome Dynamics in a Gram-Positive Bacterial Model”, MOLECULAR & CELLULAR PROTEOMICS, vol. 18, 2019, pp. 423-436.
Tsolis, K.C., Hamed, M.B., Simoens, K., Koepff, J., Busche, T., Rückert, C., Oldiges, M., Kalinowski, J., Anne, J., Kormanec, J., Bernaerts, K., Karamanou, S., Economou, A.: Secretome Dynamics in a Gram-Positive Bacterial Model. MOLECULAR & CELLULAR PROTEOMICS. 18, 423-436 (2019).
Tsolis, Konstantinos C., Hamed, Mohamed Belal, Simoens, Kenneth, Koepff, Joachim, Busche, Tobias, Rückert, Christian, Oldiges, Marco, Kalinowski, Jörn, Anne, Jozef, Kormanec, Jan, Bernaerts, Kristel, Karamanou, Spyridoula, and Economou, Anastassios. “Secretome Dynamics in a Gram-Positive Bacterial Model”. MOLECULAR & CELLULAR PROTEOMICS 18.3 (2019): 423-436.

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