Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria.

Sassi H, Nguyen TM, Telek S, Gosset G, Grünberger A, Delvigne F (2019)
Microbial biotechnology 12(5): 1064-1075.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Autor*in
Sassi, Hosni; Nguyen, Thai Minh; Telek, Samuel; Gosset, Guillermo; Grünberger, AlexanderUniBi; Delvigne, Frank
Abstract / Bemerkung
Controlling and managing the degree of phenotypic diversification of microbial populations is a challenging task. This task not only requires detailed knowledge regarding diversification mechanisms but also advanced technical set-ups for the real-time analyses and control of population behaviour on single-cell level. In this work, set-up, design and operation of the so called segregostat are described which, in contrast to a traditional chemostat, allows the control of phenotypic diversification of microbial populations over time. Two exemplary case studies will be discussed, i.e. phenotypic diversification dynamics of Eschericia coli and Pseudomonas putida based on outer membrane permeabilization, emphasizing the applicability and versatility of the proposed approach. Upon nutrient limitation, cell population tends to diversify into several subpopulations exhibiting distinct phenotypic features (non-permeabilized and permeabilized cells). Online analysis leads to the determination of the ratio between cells in these two states, which in turn triggers the addition of glucose pulses in order to maintain a predefined diversification ratio. These results prove that phenotypic diversification can be controlled by means of defined pulse-frequency modulation within continuously running bioreactor set-ups. This lays the foundation for systematic studies, not only of phenotypic diversification but also for all processes where dynamics single-cell approaches are required, such as synthetic co-culture processes. © 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
Erscheinungsjahr
2019
Zeitschriftentitel
Microbial biotechnology
Band
12
Ausgabe
5
Seite(n)
1064-1075
ISSN
1751-7915
Page URI
https://pub.uni-bielefeld.de/record/2935887

Zitieren

Sassi H, Nguyen TM, Telek S, Gosset G, Grünberger A, Delvigne F. Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria. Microbial biotechnology. 2019;12(5):1064-1075.
Sassi, H., Nguyen, T. M., Telek, S., Gosset, G., Grünberger, A., & Delvigne, F. (2019). Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria. Microbial biotechnology, 12(5), 1064-1075. doi:10.1111/1751-7915.13442
Sassi, H., Nguyen, T. M., Telek, S., Gosset, G., Grünberger, A., and Delvigne, F. (2019). Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria. Microbial biotechnology 12, 1064-1075.
Sassi, H., et al., 2019. Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria. Microbial biotechnology, 12(5), p 1064-1075.
H. Sassi, et al., “Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria.”, Microbial biotechnology, vol. 12, 2019, pp. 1064-1075.
Sassi, H., Nguyen, T.M., Telek, S., Gosset, G., Grünberger, A., Delvigne, F.: Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria. Microbial biotechnology. 12, 1064-1075 (2019).
Sassi, Hosni, Nguyen, Thai Minh, Telek, Samuel, Gosset, Guillermo, Grünberger, Alexander, and Delvigne, Frank. “Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria.”. Microbial biotechnology 12.5 (2019): 1064-1075.

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