Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study

Dargel C, Geisler R, Hannappel Y, Kemker I, Sewald N, Hellweg T (2019)
Colloids and Interfaces 3(2): 47.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
This work investigates the temperature-dependent micelle formation as well as the micellar structure of the saponin aescin. The critical micelle concentration ( cmc ) of aescin is determined from the concentration-dependent autofluorescence (AF) of aescin. Values between cmcaescin,AF (10 ∘ C) = 0.38 ± 0.09 mM and cmcaescin,AF (50 ∘ C) = 0.32 ± 0.13 mM were obtained. The significance of this method is verified by tensiometry measurements. The value determined from this method is within the experimental error identical with values obtained from autofluorescence ( cmcaescin,T(WP) (23 ∘ C) = 0.33 ± 0.02 mM). The structure of the aescin micelles was investigated by small-angle X-ray scattering (SAXS) at 10 and 40 ∘ C. At low temperature, the aescin micelles are rod-like, whereas at high temperature the structure is ellipsoidal. The radii of gyration were determined to ≈31 Å (rods) and ≈21 Å (ellipsoid). The rod-like shape of the aescin micelles at low temperature was confirmed by transmission electron microscopy (TEM). All investigations were performed at a constant pH of 7.4, because the acidic aescin has the ability to lower the pH value in aqueous solution.
Stichworte
saponin; aescin; critical micelle concentration (cmc); autofluorescence; small-angle X-ray scattering (SAXS); transmission electron microscopy (TEM); micelle structure
Erscheinungsjahr
2019
Zeitschriftentitel
Colloids and Interfaces
Band
3
Ausgabe
2
Art.-Nr.
47
eISSN
2504-5377
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2935387

Zitieren

Dargel C, Geisler R, Hannappel Y, Kemker I, Sewald N, Hellweg T. Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study. Colloids and Interfaces. 2019;3(2): 47.
Dargel, C., Geisler, R., Hannappel, Y., Kemker, I., Sewald, N., & Hellweg, T. (2019). Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study. Colloids and Interfaces, 3(2), 47. doi:10.3390/colloids3020047
Dargel, C., Geisler, R., Hannappel, Y., Kemker, I., Sewald, N., and Hellweg, T. (2019). Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study. Colloids and Interfaces 3:47.
Dargel, C., et al., 2019. Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study. Colloids and Interfaces, 3(2): 47.
C. Dargel, et al., “Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study”, Colloids and Interfaces, vol. 3, 2019, : 47.
Dargel, C., Geisler, R., Hannappel, Y., Kemker, I., Sewald, N., Hellweg, T.: Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study. Colloids and Interfaces. 3, : 47 (2019).
Dargel, Carina, Geisler, Ramsia, Hannappel, Yvonne, Kemker, Isabell, Sewald, Norbert, and Hellweg, Thomas. “Self-Assembly of the Bio-Surfactant Aescin in Solution. A Small-Angle X-ray Scattering and Fluorescence Study”. Colloids and Interfaces 3.2 (2019): 47.
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2019-04-29T06:47:51Z
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