Tailor-made catalytically active inclusion bodies for different applications in biocatalysis

Kloss R, Karmainski T, Jaeger VD, Hahn D, Grünberger A, Baumgart M, Krauss U, Jaeger K-E, Wiechert W, Pohl M (2018)
CATALYSIS SCIENCE & TECHNOLOGY 8(22): 5816-5826.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ; ; ; ; ;
Abstract / Bemerkung
We have recently demonstrated that fusions of different target enzymes to the coiled-coil domain TDoT induced the formation of catalytically active inclusion bodies (CatIBs) in E. coli (doi:10.1016/j.jbiotec.2017.04.033). Here we show that the CatIB properties can be tailored to the requirements of different reaction systems using two different coiled-coil domains as fusion tags: TDoT and 3HAMP. As an example the benzaldehyde lyase from Pseudomonas fluorescens (PfBAL) was chosen, which catalyzes the formation of 2-hydroxy ketones and benzoins from aromatic donor aldehydes and aromatic or aliphatic acceptor aldehydes. Using these fusion tags two different kinds of PfBAL-CatIBs were successfully produced that differ in morphology, solubility during washing steps, initial rate activity, protein and lipid content. TDoT-PfBAL and 3HAMP-PfBAL CatIBs were studied relative to the soluble enzyme concerning their application in biocatalysis in continuous reaction mode and in batch using a mono- and biphasic solvent system. Both CatIBs outperformed the soluble enzyme with respect to stability under reaction conditions. In the buffer/DMSO system used for the continuous synthesis of (R)-2-hydroxy-1-phenylpropanone, TDoT-PfBAL was superior to the soluble enzyme. Besides, 3HAMP-PfBAL was more suitable in a biphasic reaction system for the synthesis of (R)-benzoins and revealed an up to 3-fold higher activity compared to the soluble enzyme.
Erscheinungsjahr
Zeitschriftentitel
CATALYSIS SCIENCE & TECHNOLOGY
Band
8
Ausgabe
22
Seite(n)
5816-5826
ISSN
eISSN
PUB-ID

Zitieren

Kloss R, Karmainski T, Jaeger VD, et al. Tailor-made catalytically active inclusion bodies for different applications in biocatalysis. CATALYSIS SCIENCE & TECHNOLOGY. 2018;8(22):5816-5826.
Kloss, R., Karmainski, T., Jaeger, V. D., Hahn, D., Grünberger, A., Baumgart, M., Krauss, U., et al. (2018). Tailor-made catalytically active inclusion bodies for different applications in biocatalysis. CATALYSIS SCIENCE & TECHNOLOGY, 8(22), 5816-5826. doi:10.1039/c8cy01891j
Kloss, R., Karmainski, T., Jaeger, V. D., Hahn, D., Grünberger, A., Baumgart, M., Krauss, U., Jaeger, K. - E., Wiechert, W., and Pohl, M. (2018). Tailor-made catalytically active inclusion bodies for different applications in biocatalysis. CATALYSIS SCIENCE & TECHNOLOGY 8, 5816-5826.
Kloss, R., et al., 2018. Tailor-made catalytically active inclusion bodies for different applications in biocatalysis. CATALYSIS SCIENCE & TECHNOLOGY, 8(22), p 5816-5826.
R. Kloss, et al., “Tailor-made catalytically active inclusion bodies for different applications in biocatalysis”, CATALYSIS SCIENCE & TECHNOLOGY, vol. 8, 2018, pp. 5816-5826.
Kloss, R., Karmainski, T., Jaeger, V.D., Hahn, D., Grünberger, A., Baumgart, M., Krauss, U., Jaeger, K.-E., Wiechert, W., Pohl, M.: Tailor-made catalytically active inclusion bodies for different applications in biocatalysis. CATALYSIS SCIENCE & TECHNOLOGY. 8, 5816-5826 (2018).
Kloss, Ramona, Karmainski, Tobias, Jaeger, Vera D., Hahn, Doris, Grünberger, Alexander, Baumgart, Meike, Krauss, Ulrich, Jaeger, Karl-Erich, Wiechert, Wolfgang, and Pohl, Martina. “Tailor-made catalytically active inclusion bodies for different applications in biocatalysis”. CATALYSIS SCIENCE & TECHNOLOGY 8.22 (2018): 5816-5826.