Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions
Koepff J, Sachs CC, Wiechert W, Kohlheyer D, Noeh K, Oldiges M, Grünberger A (2018)
FRONTIERS IN MICROBIOLOGY 9: 2680.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Koepff, Joachim;
Sachs, Christian Carsten;
Wiechert, Wolfgang;
Kohlheyer, Dietrich;
Noeh, Katharina;
Oldiges, Marco;
Grünberger, AlexanderUniBi
Abstract / Bemerkung
Quantitative single-cell cultivation has provided fundamental contributions to our understanding of heterogeneity among industrially used microorganisms. Filamentous growing Streptomyces species are emerging platform organisms for industrial production processes, but their exploitation is still limited due to often reported high batch-to-batch variations and unexpected growth and production differences. Population heterogeneity is suspected to be one responsible factor, which is so far not systematically investigated at the single-cell level. Novel microfluidic single-cell cultivation devices offer promising solutions to investigate these phenomena. In this study, we investigated the germination and growth behavior of Streptomyces lividans TK24 under varying medium compositions on different complexity levels (i.e., mycelial growth, hyphal growth and tip elongation) on single-cell level. Our analysis reveals a remarkable stability within growth and germination of spores and early mycelium development when exposed to constant and defined environments. We show that spores undergo long metabolic adaptation processes of up to > 30 h to adjust to new medium conditions, rather than using a "persister" strategy as a possibility to cope with rapidly changing environments. Due to this uniform behavior, we conclude that S. lividans can be cultivated quite robustly under constant environmental conditions as provided by microfluidic cultivation approaches. Failure and non-reproducible cultivations are thus most likely to be found in less controllable larger-scale cultivation workflows and as a result of environmental gradients within large-scale cultivations.
Stichworte
Streptomyces lividans;
filamentous growth;
microfluidics;
single-cell;
cultivation;
germination;
tip elongation rate;
heterogeneity
Erscheinungsjahr
2018
Zeitschriftentitel
FRONTIERS IN MICROBIOLOGY
Band
9
Art.-Nr.
2680
ISSN
1664-302x
eISSN
1664-302X
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2932764
Zitieren
Koepff J, Sachs CC, Wiechert W, et al. Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions. FRONTIERS IN MICROBIOLOGY. 2018;9: 2680.
Koepff, J., Sachs, C. C., Wiechert, W., Kohlheyer, D., Noeh, K., Oldiges, M., & Grünberger, A. (2018). Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions. FRONTIERS IN MICROBIOLOGY, 9, 2680. doi:10.3389/fmicb.2018.02680
Koepff, Joachim, Sachs, Christian Carsten, Wiechert, Wolfgang, Kohlheyer, Dietrich, Noeh, Katharina, Oldiges, Marco, and Grünberger, Alexander. 2018. “Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions”. FRONTIERS IN MICROBIOLOGY 9: 2680.
Koepff, J., Sachs, C. C., Wiechert, W., Kohlheyer, D., Noeh, K., Oldiges, M., and Grünberger, A. (2018). Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions. FRONTIERS IN MICROBIOLOGY 9:2680.
Koepff, J., et al., 2018. Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions. FRONTIERS IN MICROBIOLOGY, 9: 2680.
J. Koepff, et al., “Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions”, FRONTIERS IN MICROBIOLOGY, vol. 9, 2018, : 2680.
Koepff, J., Sachs, C.C., Wiechert, W., Kohlheyer, D., Noeh, K., Oldiges, M., Grünberger, A.: Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions. FRONTIERS IN MICROBIOLOGY. 9, : 2680 (2018).
Koepff, Joachim, Sachs, Christian Carsten, Wiechert, Wolfgang, Kohlheyer, Dietrich, Noeh, Katharina, Oldiges, Marco, and Grünberger, Alexander. “Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions”. FRONTIERS IN MICROBIOLOGY 9 (2018): 2680.
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Daten bereitgestellt von European Bioinformatics Institute (EBI)
1 Zitation in Europe PMC
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