Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions

Yushchuk O, Ostash I, Vlasiuk I, Gren T, Luzhetskyy A, Kalinowski J, Fedorenko V, Ostash B (2018)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102(19): 8419-8428.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Streptomyces cyanogenus S136 is the only known producer of landomycin A (LaA), one of the largest glycosylated angucycline antibiotics possessing strong antiproliferative properties. There is rising interest in elucidation of mechanisms of action of landomycins, which, in turn, requires access to large quantities of the pure compounds. Overproduction of LaA has been achieved in the past through manipulation of cluster-situated regulatory genes. However, other components of the LaA biosynthetic regulatory network remain unknown. To fill this gap, we elucidated the contribution of AdpA family pleiotropic regulators in landomycin production via expression of adpA genes of different origins in S. cyanogenus S136. Overexpression of the native S. cyanogenus S136 adpA ortholog had no effect on landomycin titers. In the same time, expression of several heterologous adpA genes led to significantly increased landomycin production under different cultivation conditions. Hence, heterologous adpA genes are a useful tool to enhance or activate landomycin production by S. cyanogenus. Our ongoing research effort is focused on identification of mutations that render S. cyanogenus AdpA nonfunctional.
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Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
102
Ausgabe
19
Seite(n)
8419-8428
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eISSN
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Yushchuk O, Ostash I, Vlasiuk I, et al. Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2018;102(19):8419-8428.
Yushchuk, O., Ostash, I., Vlasiuk, I., Gren, T., Luzhetskyy, A., Kalinowski, J., Fedorenko, V., et al. (2018). Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 102(19), 8419-8428. doi:10.1007/s00253-018-9249-1
Yushchuk, O., Ostash, I., Vlasiuk, I., Gren, T., Luzhetskyy, A., Kalinowski, J., Fedorenko, V., and Ostash, B. (2018). Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102, 8419-8428.
Yushchuk, O., et al., 2018. Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 102(19), p 8419-8428.
O. Yushchuk, et al., “Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 102, 2018, pp. 8419-8428.
Yushchuk, O., Ostash, I., Vlasiuk, I., Gren, T., Luzhetskyy, A., Kalinowski, J., Fedorenko, V., Ostash, B.: Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 102, 8419-8428 (2018).
Yushchuk, Oleksandr, Ostash, Iryna, Vlasiuk, Iryna, Gren, Tetiana, Luzhetskyy, Andriy, Kalinowski, Jörn, Fedorenko, Victor, and Ostash, Bohdan. “Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.19 (2018): 8419-8428.

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