Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis

Lange J, Muench E, Mueller J, Busche T, Kalinowski J, Takors R, Blombach B (2018)
GENES 9(6): 297.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Zero-growth processes are a promising strategy for the production of reduced molecules and depict a steady transition from aerobic to anaerobic conditions. To investigate the adaptation of Corynebacterium glutamicum to altering oxygen availabilities, we conceived a triple-phase fermentation process that describes a gradual reduction of dissolved oxygen with a shift from aerobiosis via microaerobiosis to anaerobiosis. The distinct process phases were clearly bordered by the bacteria's physiologic response such as reduced growth rate, biomass substrate yield and altered yield of fermentation products. During the process, sequential samples were drawn at six points and analyzed via RNA-sequencing, for metabolite concentrations and for enzyme activities. We found transcriptional alterations of almost 50% (1421 genes) of the entire protein coding genes and observed an upregulation of fermentative pathways, a rearrangement of respiration, and mitigation of the basic cellular mechanisms such as transcription, translation and replication as a transient response related to the installed oxygen dependent process phases. To investigate the regulatory regime, 18 transcriptionally altered (putative) transcriptional regulators were deleted, but none of the deletion strains showed noticeable growth kinetics under an oxygen restricted environment. However, the described transcriptional adaptation of C. glutamicum resolved to varying oxygen availabilities provides a useful basis for future process and strain engineering.
Stichworte
Corynebacterium glutamicum; transcriptional response; aerobiosis; microaerobiosis; anaerobiosis; triple-phase process
Erscheinungsjahr
2018
Zeitschriftentitel
GENES
Band
9
Ausgabe
6
Art.-Nr.
297
ISSN
2073-4425
Page URI
https://pub.uni-bielefeld.de/record/2930282

Zitieren

Lange J, Muench E, Mueller J, et al. Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis. GENES. 2018;9(6): 297.
Lange, J., Muench, E., Mueller, J., Busche, T., Kalinowski, J., Takors, R., & Blombach, B. (2018). Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis. GENES, 9(6), 297. doi:10.3390/genes9060297
Lange, J., Muench, E., Mueller, J., Busche, T., Kalinowski, J., Takors, R., and Blombach, B. (2018). Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis. GENES 9:297.
Lange, J., et al., 2018. Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis. GENES, 9(6): 297.
J. Lange, et al., “Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis”, GENES, vol. 9, 2018, : 297.
Lange, J., Muench, E., Mueller, J., Busche, T., Kalinowski, J., Takors, R., Blombach, B.: Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis. GENES. 9, : 297 (2018).
Lange, Julian, Muench, Eugenia, Mueller, Jan, Busche, Tobias, Kalinowski, Jörn, Takors, Ralf, and Blombach, Bastian. “Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis”. GENES 9.6 (2018): 297.

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