Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis

Ludwig P, Sevin DC, Busche T, Kalinowski J, Bourdeaux F, Grininger M, Mack M (2018)
MICROBIOLOGY-SGM 164(6): 908-919.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Genes encoding dodecin proteins are present in almost 20% of archaeal and in more than 50% of bacterial genomes. Archaeal dodecins bind riboflavin (vitamin B-2), are thought to play a role in flavin homeostasis and possibly also help to protect cells from radical or oxygenic stress. Bacterial dodecins were found to bind riboflavin-5'-phosphate (also called flavin mononucleotide or FMN) and coenzyme A, but their physiological function remained unknown. In this study, we set out to investigate the relevance of dodecins for flavin metabolism and oxidative stress management in the phylogenetically related bacteria Streptomyces coelicolor and Streptomyces davawensis. Additionally, we explored the role of dodecins with regard to resistance against the antibiotic roseoflavin, a riboflavin analogue produced by S. davawensis. Our results show that the dodecin of S. davawensis predominantly binds FMN and is neither involved in roseoflavin biosynthesis nor in roseoflavin resistance. In contrast to S. davawensis, growth of S. coelicolor was not reduced in the presence of plumbagin, a compound, which induces oxidative stress. Plumbagin treatment stimulated expression of the dodecin gene in S. davawensis but not in S. coelicolor. Deletion of the dodecin gene in S. davawensis generated a recombinant strain which, in contrast to the wild-type, was fully resistant to plumbagin. Subsequent metabolome analyses revealed that the S. davawensis dodecin deletion strain exhibited a very different stress response when compared to the wild-type indicating that dodecins broadly affect cellular physiology.
Stichworte
Streptomycetes; flavins; roseoflavin; riboflavin; dodecin
Erscheinungsjahr
2018
Zeitschriftentitel
MICROBIOLOGY-SGM
Band
164
Ausgabe
6
Seite(n)
908-919
ISSN
1350-0872
eISSN
1465-2080
Page URI
https://pub.uni-bielefeld.de/record/2930276

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Ludwig P, Sevin DC, Busche T, et al. Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis. MICROBIOLOGY-SGM. 2018;164(6):908-919.
Ludwig, P., Sevin, D. C., Busche, T., Kalinowski, J., Bourdeaux, F., Grininger, M., & Mack, M. (2018). Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis. MICROBIOLOGY-SGM, 164(6), 908-919. doi:10.1099/mic.0.000662
Ludwig, P., Sevin, D. C., Busche, T., Kalinowski, J., Bourdeaux, F., Grininger, M., and Mack, M. (2018). Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis. MICROBIOLOGY-SGM 164, 908-919.
Ludwig, P., et al., 2018. Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis. MICROBIOLOGY-SGM, 164(6), p 908-919.
P. Ludwig, et al., “Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis”, MICROBIOLOGY-SGM, vol. 164, 2018, pp. 908-919.
Ludwig, P., Sevin, D.C., Busche, T., Kalinowski, J., Bourdeaux, F., Grininger, M., Mack, M.: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis. MICROBIOLOGY-SGM. 164, 908-919 (2018).
Ludwig, Petra, Sevin, Daniel C., Busche, Tobias, Kalinowski, Jörn, Bourdeaux, Florian, Grininger, Martin, and Mack, Matthias. “Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis”. MICROBIOLOGY-SGM 164.6 (2018): 908-919.

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