Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation

Blifernez-Klassen O, Chaudhari S, Klassen V, Wördenweber R, Steffens T, Cholewa D, Niehaus K, Kalinowski J, Kruse O (2018)
PLOS ONE 13(6): 23.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 5.29 MB
Abstract / Bemerkung
The microalga Botryococcus braunii is widely regarded as a potential renewable and sustainable source for industrial applications because of its capability to produce large amounts of metabolically expensive (exo-) polysaccharides and lipids, notably hydrocarbons. A comprehensive and systematic metabolic characterization of the Botryococcus braunii race A strain CCAP 807/2 was conducted within the present study, including the detailed analysis of growth-associated and physiological parameters. In addition, the intracellular metabolome was profiled for the first time and showed growth- and product-specific fluctuations in response to the different availability of medium resources during the cultivation course. Among the identified metabolites, a constant expression of raffinose was observed for the first time under standard conditions, which has until now only been described for higher plants. Overall, the multilayered analysis during the cultivation of strain CCAP 807/2 allowed the differentiation of four distinct physiological growth phases and revealed differences in the production profiles and content of liquid hydrocarbons and carbohydrates with up to 84% of organic dry weight (oDW). In the process, an enhanced production of carbohydrates with up to 63% of oDW (1.36 +/- 0.03 g L-1) could be observed during the late linear growth phase, whereas the highest accumulation of extracellular hydrocarbons with up to 24% of oDW (0.66 +/- 0.12 g L-1) occurred mainly during the stationary growth phase. Altogether, the knowledge obtained is potentially useful for the general understanding of the overall physiology of Botryococcus braunii and provide important insights into the growth behavior and product formation of this microalga, and is thus relevant for large scale biofuel production and industrial applications.
Erscheinungsjahr
2018
Zeitschriftentitel
PLOS ONE
Band
13
Ausgabe
6
Art.-Nr.
23
ISSN
1932-6203
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2920943

Zitieren

Blifernez-Klassen O, Chaudhari S, Klassen V, et al. Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation. PLOS ONE. 2018;13(6): 23.
Blifernez-Klassen, O., Chaudhari, S., Klassen, V., Wördenweber, R., Steffens, T., Cholewa, D., Niehaus, K., et al. (2018). Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation. PLOS ONE, 13(6), 23. doi:10.1371/journal.pone.0198976
Blifernez-Klassen, O., Chaudhari, S., Klassen, V., Wördenweber, R., Steffens, T., Cholewa, D., Niehaus, K., Kalinowski, J., and Kruse, O. (2018). Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation. PLOS ONE 13:23.
Blifernez-Klassen, O., et al., 2018. Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation. PLOS ONE, 13(6): 23.
O. Blifernez-Klassen, et al., “Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation”, PLOS ONE, vol. 13, 2018, : 23.
Blifernez-Klassen, O., Chaudhari, S., Klassen, V., Wördenweber, R., Steffens, T., Cholewa, D., Niehaus, K., Kalinowski, J., Kruse, O.: Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation. PLOS ONE. 13, : 23 (2018).
Blifernez-Klassen, Olga, Chaudhari, Swapnil, Klassen, Viktor, Wördenweber, Robin, Steffens, Tim, Cholewa, Dominik, Niehaus, Karsten, Kalinowski, Jörn, and Kruse, Olaf. “Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation”. PLOS ONE 13.6 (2018): 23.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:59Z
MD5 Prüfsumme
8eb7096f028ae5670e937c25bfa3ffe7

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 29879215
PubMed | Europe PMC

Suchen in

Google Scholar