Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production

Kloss R, Limberg MH, Mackfeld U, Hahn D, Grünberger A, Jaeger VD, Krauss U, Oldiges M, Pohl M (2018)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Kloss, Ramona; Limberg, Michael H.; Mackfeld, Ursula; Hahn, Doris; Grünberger, AlexanderUniBi; Jaeger, Vera D.; Krauss, Ulrich; Oldiges, Marco; Pohl, Martina
Abstract / Bemerkung
Sustainable and eco-efficient alternatives for the production of platform chemicals, fuels and chemical building blocks require the development of stable, reusable and recyclable biocatalysts. Here we present a novel concept for the biocatalytic production of 1,5-diaminopentane (DAP, trivial name: cadaverine) using catalytically active inclusion bodies (CatIBs) of the constitutive L-lysine decarboxylase from E. coli (EcLDCc-CatIBs) to process L-lysine-containing culture supernatants from Corynebacterium glutamicum. EcLDCc-CatIBs can easily be produced in E. coli followed by a simple purification protocol yielding up to 43% dry CatIBs per dry cell weight. The stability and recyclability of EcLDCc-CatIBs was demonstrated in (repetitive) batch experiments starting from L-lysine concentrations of 0.1 M and 1 M. EcLDC-CatIBs exhibited great stability under reaction conditions with an estimated half-life of about 54 h. High conversions to DAP of 87-100% were obtained in 30-60 ml batch reactions using approx. 180-300 mg EcLDCc-CatIBs, respectively. This resulted in DAP titres of up to 88.4 g l(-1) and space-time yields of up to 660 gDAP l(-1) d(-1) per gram dry EcLDCc-CatIBs. The new process for DAP production can therefore compete with the currently best fermentative process as described in the literature.
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Kloss R, Limberg MH, Mackfeld U, et al. Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production. SCIENTIFIC REPORTS. 2018;8(1): 5856.
Kloss, R., Limberg, M. H., Mackfeld, U., Hahn, D., Grünberger, A., Jaeger, V. D., Krauss, U., et al. (2018). Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production. SCIENTIFIC REPORTS, 8(1), 5856. doi:10.1038/s41598-018-24070-2
Kloss, R., Limberg, M. H., Mackfeld, U., Hahn, D., Grünberger, A., Jaeger, V. D., Krauss, U., Oldiges, M., and Pohl, M. (2018). Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production. SCIENTIFIC REPORTS 8:5856.
Kloss, R., et al., 2018. Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production. SCIENTIFIC REPORTS, 8(1): 5856.
R. Kloss, et al., “Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production”, SCIENTIFIC REPORTS, vol. 8, 2018, : 5856.
Kloss, R., Limberg, M.H., Mackfeld, U., Hahn, D., Grünberger, A., Jaeger, V.D., Krauss, U., Oldiges, M., Pohl, M.: Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production. SCIENTIFIC REPORTS. 8, : 5856 (2018).
Kloss, Ramona, Limberg, Michael H., Mackfeld, Ursula, Hahn, Doris, Grünberger, Alexander, Jaeger, Vera D., Krauss, Ulrich, Oldiges, Marco, and Pohl, Martina. “Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production”. SCIENTIFIC REPORTS 8.1 (2018): 5856.

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