Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum

Ruwe M, Rückert C, Kalinowski J, Persicke M (2018)
Frontiers in Microbiology 9: 916.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 2.22 MB
Abstract / Bemerkung
The (pp)pGpp metabolism is an important component of bacterial physiology as it is involved in various stress responses and mechanisms of cell homeostasis, e.g., the regulation of growth. However, in order to better understand the (pp)pGpp associated regulation, it is crucial to study the molecular mechanisms of (pp)pGpp metabolism. In recent years, bioinformatic analyses of the RelA/SpoT homolog (RSH) superfamily have led to the discovery of small monofunctional RSH derivatives in addition to the well-known bifunctional Rel proteins. These are also referred to as small alarmone synthetases (SASs) or small alarmone hydrolases (SAHs). In this study, the ORF cg1485 from C. glutamicum was identified as a putative SAH encoding gene, based on a high similarity of the corresponding amino acid sequence with the (pp)pGpp hydrolysis domain. The characterization of its gene product, designated as RelHCg, represents the first functional investigation of a bacterial representative of the SAH subfamily. The predicted pyrophosphohydrolase activity was demonstrated in vivo by expression in two E. coli strains, characterized by different alarmone basal levels, as well as by in vitro analysis of the purified protein. During the assay-based analysis of hydrolysis activity in relation to the three known alarmone species, both RelHCg and the bifunctional RSH enzyme RelCg were found to exhibit a pronounced substrate inhibition for alarmone concentrations of more than 0.75 mM. This characteristic of (pp)pGpp hydrolases could be an important mechanism for realizing the bistable character of the (pp)pGpp metabolism between a (pp)pGpp basal level and stress-associated alarmone production. The deletion of relHCg caused only a minor effect on growth behavior in both wild-type background and deletion mutants with deletion of (pp)pGpp synthetases. Based on this observation, the protein is probably only present or active under specific environmental conditions. The independent loss of the corresponding gene in numerous representatives of the genus Corynebacterium, which was found by bioinformatic analyses, also supports this hypothesis. Furthermore, growth analysis of all possible deletion combinations of the three active C. glutamicum RSH genes revealed interesting functional relationships which will have to be investigated in more detail in the future.
Erscheinungsjahr
2018
Zeitschriftentitel
Frontiers in Microbiology
Band
9
Art.-Nr.
916
ISSN
1664-302X
Page URI
https://pub.uni-bielefeld.de/record/2920133

Zitieren

Ruwe M, Rückert C, Kalinowski J, Persicke M. Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum. Frontiers in Microbiology. 2018;9: 916.
Ruwe, M., Rückert, C., Kalinowski, J., & Persicke, M. (2018). Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum. Frontiers in Microbiology, 9, 916. doi:10.3389/fmicb.2018.00916
Ruwe, M., Rückert, C., Kalinowski, J., and Persicke, M. (2018). Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum. Frontiers in Microbiology 9:916.
Ruwe, M., et al., 2018. Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum. Frontiers in Microbiology, 9: 916.
M. Ruwe, et al., “Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum”, Frontiers in Microbiology, vol. 9, 2018, : 916.
Ruwe, M., Rückert, C., Kalinowski, J., Persicke, M.: Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum. Frontiers in Microbiology. 9, : 916 (2018).
Ruwe, Matthias, Rückert, Christian, Kalinowski, Jörn, and Persicke, Marcus. “Functional characterization of a small Alarmone Hydrolase in Corynebacterium glutamicum”. Frontiers in Microbiology 9 (2018): 916.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:59Z
MD5 Prüfsumme
85e71103f220e56c05231531f6538424

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 29867827
PubMed | Europe PMC

Suchen in

Google Scholar