LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii

Cerletti M, Paggi R, Troetschel C, Celeste Ferrari M, Guevara CR, Albaum S, Poetsch A, De Castro R (2018)
JOURNAL OF PROTEOME RESEARCH 17(3): 1158-1171.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The membrane protease LonB is an essential protein in the archaeon Haloferax volcanii and globally impacts its physiology. However, natural substrates of the archaeal Lon protease have not been identified. The whole proteome turnover was examined in a H. volcanii LonB mutant under reduced and physiological protease levels. LC-MS/MS combined with stable isotope labeling was applied for the identification/quantitation of membrane and cytoplasm proteins. Differential synthesis and degradation rates were evidenced for 414 proteins in response to Lon expression. A total of 58 proteins involved in diverse cellular processes showed a degradation pattern (none/very little degradation in the absence of Lon and increased degradation in the presence of Lon) consistent with a LonB substrate, which was further substantiated for several of these candidates by pull-down assays. The most notable was phytoene synthase (PSY), the rate-limiting enzyme in carotenoid biosynthesis. The rapid degradation of PSY upon LonB induction in addition to the remarkable stabilization of this protein and hyperpigmentation phenotype in the Lon mutant strongly suggest that PSY is a LonB substrate. This work identifies for the first time candidate targets of the archaeal Lon protease and establishes proteolysis by Lon as a novel post-translational regulatory mechanism of carotenogenesis.
Stichworte
membrane protease; proteome turnover; LonB substrates; Haloferax; volcanii; phytoene synthase; Archaea
Erscheinungsjahr
2018
Zeitschriftentitel
JOURNAL OF PROTEOME RESEARCH
Band
17
Ausgabe
3
Seite(n)
1158-1171
ISSN
1535-3893
eISSN
1535-3907
Page URI
https://pub.uni-bielefeld.de/record/2919065

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Cerletti M, Paggi R, Troetschel C, et al. LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. JOURNAL OF PROTEOME RESEARCH. 2018;17(3):1158-1171.
Cerletti, M., Paggi, R., Troetschel, C., Celeste Ferrari, M., Guevara, C. R., Albaum, S., Poetsch, A., et al. (2018). LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. JOURNAL OF PROTEOME RESEARCH, 17(3), 1158-1171. doi:10.1021/acs.jproteome.7b00809
Cerletti, M., Paggi, R., Troetschel, C., Celeste Ferrari, M., Guevara, C. R., Albaum, S., Poetsch, A., and De Castro, R. (2018). LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. JOURNAL OF PROTEOME RESEARCH 17, 1158-1171.
Cerletti, M., et al., 2018. LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. JOURNAL OF PROTEOME RESEARCH, 17(3), p 1158-1171.
M. Cerletti, et al., “LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii”, JOURNAL OF PROTEOME RESEARCH, vol. 17, 2018, pp. 1158-1171.
Cerletti, M., Paggi, R., Troetschel, C., Celeste Ferrari, M., Guevara, C.R., Albaum, S., Poetsch, A., De Castro, R.: LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. JOURNAL OF PROTEOME RESEARCH. 17, 1158-1171 (2018).
Cerletti, Micaela, Paggi, Roberto, Troetschel, Christian, Celeste Ferrari, Maria, Guevara, Carina Ramallo, Albaum, Stefan, Poetsch, Ansgar, and De Castro, Rosana. “LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii”. JOURNAL OF PROTEOME RESEARCH 17.3 (2018): 1158-1171.

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