Visualization of tandem repeat mutagenesis in Bacillus subtilis

Dormeyer M, Lentes S, Ballin P, Wilkens M, Klumpp S, Kohlheyer D, Stannek L, Grünberger A, Commichau FM (2018)
DNA REPAIR 63: 10-15.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Dormeyer, Miriam; Lentes, Sabine; Ballin, Patrick; Wilkens, Markus; Klumpp, Stefan; Kohlheyer, Dietrich; Stannek, Lorena; Grünberger, AlexanderUniBi; Commichau, Fabian M.
Abstract / Bemerkung
Mutations are crucial for the emergence and evolution of proteins with novel functions, and thus for the diversity of life. Tandem repeats (TRs) are mutational hot spots that are present in the genomes of all organisms. Understanding the molecular mechanism underlying TR mutagenesis at the level of single cells requires the development of mutation reporter systems. Here, we present a mutation reporter system that is suitable to visualize mutagenesis of TRs occurring in single cells of the Gram-positive model bacterium Bacillus subtilis using microfluidic single-cell cultivation. The system allows measuring the elimination of TR units due to growth rate recovery. The cultivation of bacteria carrying the mutation reporter system in microfluidic chambers allowed us for the first time to visualize the emergence of a specific mutation at the level of single cells. The application of the mutation reporter system in combination with microfluidics might be helpful to elucidate the molecular mechanism underlying TR (in)stability in bacteria. Moreover, the mutation reporter system might be useful to assess whether mutations occur in response to nutrient starvation.
Stichworte
Bacillus subtilis; Mutagenesis; Mutational hot spot; Tandem repeat; Reporter system; Microfluidics
Erscheinungsjahr
2018
Zeitschriftentitel
DNA REPAIR
Band
63
Seite(n)
10-15
ISSN
1568-7864
eISSN
1568-7856
Page URI
https://pub.uni-bielefeld.de/record/2919063

Zitieren

Dormeyer M, Lentes S, Ballin P, et al. Visualization of tandem repeat mutagenesis in Bacillus subtilis. DNA REPAIR. 2018;63:10-15.
Dormeyer, M., Lentes, S., Ballin, P., Wilkens, M., Klumpp, S., Kohlheyer, D., Stannek, L., et al. (2018). Visualization of tandem repeat mutagenesis in Bacillus subtilis. DNA REPAIR, 63, 10-15. doi:10.1016/j.dnarep.2018.01.006
Dormeyer, Miriam, Lentes, Sabine, Ballin, Patrick, Wilkens, Markus, Klumpp, Stefan, Kohlheyer, Dietrich, Stannek, Lorena, Grünberger, Alexander, and Commichau, Fabian M. 2018. “Visualization of tandem repeat mutagenesis in Bacillus subtilis”. DNA REPAIR 63: 10-15.
Dormeyer, M., Lentes, S., Ballin, P., Wilkens, M., Klumpp, S., Kohlheyer, D., Stannek, L., Grünberger, A., and Commichau, F. M. (2018). Visualization of tandem repeat mutagenesis in Bacillus subtilis. DNA REPAIR 63, 10-15.
Dormeyer, M., et al., 2018. Visualization of tandem repeat mutagenesis in Bacillus subtilis. DNA REPAIR, 63, p 10-15.
M. Dormeyer, et al., “Visualization of tandem repeat mutagenesis in Bacillus subtilis”, DNA REPAIR, vol. 63, 2018, pp. 10-15.
Dormeyer, M., Lentes, S., Ballin, P., Wilkens, M., Klumpp, S., Kohlheyer, D., Stannek, L., Grünberger, A., Commichau, F.M.: Visualization of tandem repeat mutagenesis in Bacillus subtilis. DNA REPAIR. 63, 10-15 (2018).
Dormeyer, Miriam, Lentes, Sabine, Ballin, Patrick, Wilkens, Markus, Klumpp, Stefan, Kohlheyer, Dietrich, Stannek, Lorena, Grünberger, Alexander, and Commichau, Fabian M. “Visualization of tandem repeat mutagenesis in Bacillus subtilis”. DNA REPAIR 63 (2018): 10-15.

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