Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites

Sun Y-Q, Busche T, Rückert C, Paulus C, Rebets Y, Novakova R, Kalinowski J, Luzhetskyy A, Kormanec J, Sekurova ON, Zotchev SB (2017)
ACS SYNTHETIC BIOLOGY 6(6): 1026-1033.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Genome mining of actinomycete bacteria aims at the discovery of novel bioactive secondary metabolites that can be developed into drugs. A new repressor based biosensor to detect activated secondary metabolite biosynthesis gene clusters in Streptomyces was developed. Biosynthetic gene clusters for undecylprodigiosin and coelimycin in the genome of Streptomyces lividans TK24, which encoded TetR-like repressors and appeared to be almost "silent" based on the RNA-seq data, were chosen for the proof-of-principle studies. The bpsA reporter gene for indigoidine synthetase was placed under control of the promotor/operator regions presumed to be controlled by the cluster-associated TetR-like repressors. While the biosensor for undecylprodigiosin turned out to be nonfunctional, the coelimycin biosensor was shown to perform as expected, turning on biosynthesis of indigoidine in response to the concomitant production of coelimycin. The developed reporter system concept can be applied to those cryptic gene clusters that encode metabolite-sensing repressors to speed up discovery of novel bioactive compounds in Streptomyces.
Erscheinungsjahr
Zeitschriftentitel
ACS SYNTHETIC BIOLOGY
Band
6
Ausgabe
6
Seite(n)
1026-1033
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Sun Y-Q, Busche T, Rückert C, et al. Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites. ACS SYNTHETIC BIOLOGY. 2017;6(6):1026-1033.
Sun, Y. - Q., Busche, T., Rückert, C., Paulus, C., Rebets, Y., Novakova, R., Kalinowski, J., et al. (2017). Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites. ACS SYNTHETIC BIOLOGY, 6(6), 1026-1033. doi:10.1021/acssynbio.6b00353
Sun, Y. - Q., Busche, T., Rückert, C., Paulus, C., Rebets, Y., Novakova, R., Kalinowski, J., Luzhetskyy, A., Kormanec, J., Sekurova, O. N., et al. (2017). Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites. ACS SYNTHETIC BIOLOGY 6, 1026-1033.
Sun, Y.-Q., et al., 2017. Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites. ACS SYNTHETIC BIOLOGY, 6(6), p 1026-1033.
Y.-Q. Sun, et al., “Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites”, ACS SYNTHETIC BIOLOGY, vol. 6, 2017, pp. 1026-1033.
Sun, Y.-Q., Busche, T., Rückert, C., Paulus, C., Rebets, Y., Novakova, R., Kalinowski, J., Luzhetskyy, A., Kormanec, J., Sekurova, O.N., Zotchev, S.B.: Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites. ACS SYNTHETIC BIOLOGY. 6, 1026-1033 (2017).
Sun, Yi-Qian, Busche, Tobias, Rückert, Christian, Paulus, Constanze, Rebets, Yuriy, Novakova, Renata, Kalinowski, Jörn, Luzhetskyy, Andriy, Kormanec, Jan, Sekurova, Olga N., and Zotchev, Sergey B. “Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites”. ACS SYNTHETIC BIOLOGY 6.6 (2017): 1026-1033.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Toward Systems Metabolic Engineering of Streptomycetes for Secondary Metabolites Production.
Robertsen HL, Weber T, Kim HU, Lee SY., Biotechnol J 13(1), 2018
PMID: 29076639
Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era.
van der Heul HU, Bilyk BL, McDowall KJ, Seipke RF, van Wezel GP., Nat Prod Rep 35(6), 2018
PMID: 29721572
Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp. NP10.
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PMID: 29971051
Boosting Secondary Metabolite Production and Discovery through the Engineering of Novel Microbial Biosensors.
de Frias UA, Pereira GKB, Guazzaroni ME, Silva-Rocha R., Biomed Res Int 2018(), 2018
PMID: 30079350
Activation of microbial secondary metabolic pathways: Avenues and challenges.
Baral B, Akhgari A, Metsä-Ketelä M., Synth Syst Biotechnol 3(3), 2018
PMID: 30345402

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